Androgen Receptor and Vasopressin Receptor (AVPR1a) Genetic Polymorphisms are not associated with Marital Status or Fertility among Ariaal Men of Northern Kenya

A growing body of scholarship implicates testosterone and vasopressin in male reproductive behavior, including in humans. Since hormones exert their effects through their respective receptors, an open question has been whether genetic polymorphisms in the androgen receptor and vasopressin 1a receptor (AVPR1a) impact human male social behavior. Here, we sought to test for associations between polymorphisms in the coding region of the androgen receptor and promoter region of AVPR1a in relation to marital status and fertility among pastoralist Ariaal men of northern Kenya. None of the three polymorphisms were related to marital status (single, monogamously married, polygynously married) or fertility (number of current living children). We discuss these null findings in light of existing data

A model for predicting dissolved organic carbon distribution in a reservoir water using fluorescence spectroscopy

A number of water treatment works (WTW) in the north of England (UK) have experienced problems in reducing the dissolved organic carbon (DOC) present in the water to a sufficiently low level. The problems are experienced in autumn/ winter when the colour increases and the coagulant dose at the WTW needs to be increased in order to achieve sufficient colour removal. However, the DOC content of the water varies little throughout the year. To investigate this further, the water was fractionated using resin adsorption techniques into its hydrophobic (fulvic and humic acid fractions) and hydrophilic (acid and non-acid fractions) components. The fractionation process yields useful information on the changing concentration of each fraction but is time consuming and labour intensive. Here, a method of rapidly determining fraction concentration was developed using fluorescence spectroscopy. The model created used synchronous spectra of fractionated material compared against bulk water spectra and predicted the fraction concentrations to within 10% for a specific water. The model was unable to predict fraction concentrations for waters from a different watershed

Androgen Receptor CAG Repeats and Body Composition Among Ariaal Men

To determine the population variation in the androgen receptor (AR) and its association with body composition in a subsistence population, we sampled 87 settled and 65 nomadic males ages 20+ among the Ariaal of northern Kenya. Anthropometric measures included height, body mass index, fat-free mass (FFM), upper arm muscle plus bone area (AMPBA), % body fat (%BF), suprailliac skinfold (SISF), and waist-to-hip ratio. Salivary testosterone (T) was determined from both morning (Am T) and afternoon (Pm T) samples. Hair roots were obtained for genotyping AR CAG repeat length. AR CAG repeat length did not vary between the two sub-groups (overall value = 22.6 ± 3.1). Multiple regression models, controlling for age and residence, indicate that Pm T was positively associated with all measures of body composition. AR CAG repeat length was a significant positive predictor of height, FFM, %BF, SISF and waist circumference. There was a significant negative Pm T by AR CAG repeat length interaction in predicting all anthropometric measures but AMPBA. These findings provide evidence for population variation in AR CAG repeat length and suggest that both T and AR CAG length play a role in body composition in this extremely lean population.AnthropologyHuman Evolutionary Biolog

Closing the Nuclear Fuel Cycle with a Simplified Minor Actinide Lanthanide Separation Process (ALSEP) and Additive Manufacturing

Expanded low-carbon baseload power production through the use of nuclear fission can be enabled by recycling long-lived actinide isotopes within the nuclear fuel cycle. This approach provides the benefits of (a) more completely utilizing the energy potential of mined uranium, (b) reducing the footprint of nuclear geological repositories, and (c) reducing the time required for the radiotoxicity of the disposed waste to decrease to the level of uranium ore from one hundred thousand years to a few hundred years. A key step in achieving this goal is the separation of long-lived isotopes of americium (Am) and curium (Cm) for recycle into fast reactors. To achieve this goal, a novel process was successfully demonstrated on a laboratory scale using a bank of 1.25-cm centrifugal contactors, fabricated by additive manufacturing, and a simulant containing the major fission product elements. Americium and Cm were separated from the lanthanides with over 99.9% completion. The sum of the impurities of the Am/Cm product stream using the simulated raffinate was found to be 3.2 × 10−3 g/L. The process performance was validated using a genuine high burnup used nuclear fuel raffinate in a batch regime. Separation factors of nearly 100 for 154Eu over 241Am were achieved. All these results indicate the process scalability to an engineering scale

Development of Possible Go-Around Criteria for Transport Aircraft

This paper adds data to help with the development of possible go-around criteria for transport category aircraft. Presently, airline procedures state that pilots make a go-around decision using multiple criteria at 1000 or 500 ft above the ground, or so-called gates. An experiment conducted on three level-D full-flight simulators investigated the conditions from which pilots cannot successfully recover from an unstable approach and make a normal landing. In addition, pilots' perceptions of risk under these various unstable approach conditions and resulting landings were assessed. Six crews, comprised of a captain and a first officer from the same airline, participated in each simulator. Both the captain and the first officer flew approaches and landings from 55 different initial conditions with varying gate heights, localizer deviations, glideslope deviations, reference-speed deviations, and rate of descents. The initial condition at the starting gate mainly affected longitudinal touchdown deviation and rate of descent at touchdown, with reference-speed deviation having the most significant effect. Results show little difference in touchdown performance for conditions from the 300-ft and 500-ft gates. Conditions at the 100-ft gate introduced significant differences in touchdown performance. Reference-speed and localizer deviation at the starting gate had the strongest influences on perceived risk and go-around decision. In line with other studies, these findings suggest that a 300-ft go-around gate might be acceptable. More research is required to investigate the effects of environmental and runway variables before possible go-around criteria for transport category aircraft can be defined

Pilot Evaluation of Proposed Go-Around Criteria for Transport Category Aircraft

The primary objective of this study was to capture pilot feedback and decision-making with regard to proposed, hypothetical, go-around criteria that were developed based on previous research. A secondary objective of the study was to assess crew members' awareness of the aircraft state on approach. An experiment was conducted using Boeing 737-800 and Airbus A330-200 level D full-flight simulators, in which pilots flew multiple approaches which were on the borderline of the proposed go-around criteria at 300 ft. Pilots were instructed that they could either execute a go-around or land the airplane on each run, forcing a decision for the borderline cases at 300 ft. Pilots were instructed to go around if the aircraft was outside of the go-around criteria at 300 ft or if either pilot was uncomfortable with the approach. The results revealed that: 1) the most important factors which drove go-around decision-making during the experiment were airspeed and localizer deviation, 2) the objective data suggested that the 300-ft gate is viable, although many pilots were still uncomfortable with that gate height; perhaps more emphasis on checking stability at 1,000 ft and 500 ft would make more pilots comfortable with the 300-ft go-around gate, 3) allowing for momentary deviations should be considered, and 4) the acceptability of the criteria is highly dependent on given pilot's risk tolerance. Overall, the proposed criteria performed well, and most pilots would find the criteria acceptable with some minor adjustments

Irreparable massive rotator cuff tear in a young patient with recurrent anterior shoulder dislocation

Combined latissimus dorsi transfer, subscapularis repair and Latarjet surgery is rare and has never been reported. A 35-year-old man with chronic shoulder pain had a long history of instability of his right shoulder. The first episode occurred during a game of touch rugby followed by multiple episodes of subluxation. MRI was done which showed complete tear of the subscapularis anteriorly which was retracted and atrophied indicating a longstanding tear. There was also significant mid substance supraspinatus tendon tear. Patient then underwent two surgeries. The initial surgery found the rotator cuff to be irreparable with glenoid bone loss and only acromioplasty with acromioclavicular joint resection were performed. He then had a single stage surgery consisting of latissimus dorsi transfer, Latarjet procedure and subscapularis repair. A two-stage surgery can be avoided, and good results can be obtained provided that the patient undergo correct rehabilitation regime after undergoing a single stage surgery

Time-lapse ultrashort pulse microscopy of infection in three-dimensional versus two-dimensional culture environments reveals enhanced extra-chromosomal virus replication compartment formation

The mechanisms that enable viruses to harness cellular machinery for their own survival are primarily studied in cell lines cultured in two-dimensional (2-D) environments. However, there are increasing reports of biological differences between cells cultured in 2-D versus three-dimensional (3-D) environments. Here we report differences in host-virus interactions based on differences in culture environment. Using ultrashort pulse microscopy (UPM), a form of two-photon microscopy that utilizes sub-10-fs pulses to efficiently excite fluorophores, we have shown that de novo development of extra-chromosomal virus replication compartments (VRCs) upon murine cytomegalovirus (mCMV) infection is markedly enhanced when host cells are cultured in 3-D collagen gels versus 2-D monolayers. In addition, time-lapse imaging revealed that mCMV-induced VRCs have the capacity to grow by coalescence. This work supports the future potential of 3-D culture as a useful bridge between traditional monolayer cultures and animal models to study host-virus interactions in a more physiologically relevant environment for the development of effective anti-viral therapeutics. These advances will require broader adoption of modalities, such as UPM, to image deep within scattering tissues

The Structural Basis of Actin Organization by Vinculin and Metavinculin

Vinculin is an essential adhesion protein that links membrane-bound integrin and cadherin receptors through their intracellular binding partners to filamentous actin, facilitating mechanotransduction. Here we present an 8.5-Å-resolution cryo-electron microscopy reconstruction and pseudo-atomic model of the vinculin tail (Vt) domain bound to F-actin. Upon actin engagement, the N-terminal "strap" and helix 1 are displaced from the Vt helical bundle to mediate actin bundling. We find that an analogous conformational change also occurs in the H1' helix of the tail domain of metavinculin (MVt) upon actin binding, a muscle-specific splice isoform that suppresses actin bundling by Vt. These data support a model in which metavinculin tunes the actin bundling activity of vinculin in a tissue-specific manner, providing a mechanistic framework for understanding metavinculin mutations associated with hereditary cardiomyopathies

Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs

The acute phase protein response is a well-described generalized early host response to tissue injury, inflammation and infection, observed as pronounced changes in the concentrations of a number of circulating serum proteins. The biological function of this response and its interplay with other parts of innate host defence reactions remain somewhat elusive. In order to gain new insight into this early host defence response in the context of bacterial infection we studied gene expression changes in peripheral lymphoid tissues as compared to hepatic expression changes, 14–18 h after lung infection in pigs. The lung infection was established with the pig specific respiratory pathogen Actinobacillus pleuropneumoniae. Quantitative real-time PCR based expression analysis were performed on samples from liver, tracheobronchial lymph node, tonsils, spleen and on blood leukocytes, supplemented with measurements of interleukin-6 and selected acute phase proteins in serum. C-reactive protein and serum amyloid A were clearly induced 14–18 h after infection. Extrahepatic expression of acute phase proteins was found to be dramatically altered as a result of the lung infection with an extrahepatic acute phase protein response occurring concomitantly with the hepatic response. This suggests that the acute phase protein response is a more disseminated systemic response than previously thought. The current study provides to our knowledge the first example of porcine extrahepatic expression and regulation of C-reactive protein, haptoglobin, fibrinogen, pig major acute phase protein, and transferrin in peripheral lymphoid tissues