From mounds to landscapes: the transformation processes and the construction of landscapes in southern La Plata basin

A través de un estudio comparado, se analiza el rol que tuvo la construcción de montículos en la configuración de dos tipos particulares de paisajes arqueológicos: las estructuras monumentales (cerritos) de los humedales del noreste de Uruguay y los montículos antrópicos de las islas y ambientes litorales del Delta del río Paraná, Argentina. Sobre la base de los resultados de las prospecciones y excavaciones arqueológicas en estructuras monticulares de ambas regiones, se discute el origen de estas manifestaciones prehispánicas, los procesos de formación de sitio y las actividades implicadas, así como el desarrollo y conformación de estos paisajes arqueológicos. El análisis de las regularidades y las diferencias entre ambas experiencias regionales se integran en una discusión más amplia sobre la ocupación y trayectoria histórica de las tierras bajas sudamericanas.Through a comparative study, we analyze the role of mound building in the configuration of two particular types of archaeological landscapes: the monumental earthen structures (cerritos) of the wetlands of Northeast Uruguay and the human-made earthen mounds of the islands and littoral environments of the Delta of the Paraná River, Argentina. Based on the results of archaeologica surveys and excavations of mound sites from both regions, the origin of these pre-Hispanic manifestations, the site formation processes and activities involved, and the development and shaping of these archaeological landscapes are discussed. The analysis of the regularities and differences between the two regional experiences are integrated into a broader discussion about the occupation and historical trajectory of Lowland South America.Fil: Gianotti, Camila. Universidad de la Republica; UruguayFil: Bonomo, Mariano. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División Arqueologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

From mounds to landscapes: the transformation processes and the construction of landscapes in southern La Plata basin

A través de un estudio comparado, se analiza el rol que tuvo la construcción de montículos en la configuración de dos tipos particulares de paisajes arqueológicos: las estructuras monumentales (cerritos) de los humedales del noreste de Uruguay y los montículos antrópicos de las islas y ambientes litorales del Delta del río Paraná, Argentina. Sobre la base de los resultados de las prospecciones y excavaciones arqueológicas en estructuras monticulares de ambas regiones, se discute el origen de estas manifestaciones prehispánicas, los procesos de formación de sitio y las actividades implicadas, así como el desarrollo y conformación de estos paisajes arqueológicos. El análisis de las regularidades y las diferencias entre ambas experiencias regionales se integran en una discusión más amplia sobre la ocupación y trayectoria histórica de las tierras bajas sudamericanas.Through a comparative study, we analyze the role of mound building in the configuration of two particular types of archaeological landscapes: the monumental earthen structures (cerritos) of the wetlands of Northeast Uruguay and the human-made earthen mounds of the islands and littoral environments of the Delta of the Paraná River, Argentina. Based on the results of archaeologica surveys and excavations of mound sites from both regions, the origin of these pre-Hispanic manifestations, the site formation processes and activities involved, and the development and shaping of these archaeological landscapes are discussed. The analysis of the regularities and differences between the two regional experiences are integrated into a broader discussion about the occupation and historical trajectory of Lowland South America.Facultad de Ciencias Naturales y Muse

From mounds to landscapes: the transformation processes and the construction of landscapes in southern La Plata basin

A través de un estudio comparado, se analiza el rol que tuvo la construcción de montículos en la configuración de dos tipos particulares de paisajes arqueológicos: las estructuras monumentales (cerritos) de los humedales del noreste de Uruguay y los montículos antrópicos de las islas y ambientes litorales del Delta del río Paraná, Argentina. Sobre la base de los resultados de las prospecciones y excavaciones arqueológicas en estructuras monticulares de ambas regiones, se discute el origen de estas manifestaciones prehispánicas, los procesos de formación de sitio y las actividades implicadas, así como el desarrollo y conformación de estos paisajes arqueológicos. El análisis de las regularidades y las diferencias entre ambas experiencias regionales se integran en una discusión más amplia sobre la ocupación y trayectoria histórica de las tierras bajas sudamericanas.Through a comparative study, we analyze the role of mound building in the configuration of two particular types of archaeological landscapes: the monumental earthen structures (cerritos) of the wetlands of Northeast Uruguay and the human-made earthen mounds of the islands and littoral environments of the Delta of the Paraná River, Argentina. Based on the results of archaeologica surveys and excavations of mound sites from both regions, the origin of these pre-Hispanic manifestations, the site formation processes and activities involved, and the development and shaping of these archaeological landscapes are discussed. The analysis of the regularities and differences between the two regional experiences are integrated into a broader discussion about the occupation and historical trajectory of Lowland South America.Facultad de Ciencias Naturales y Muse

Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain

The human pathogen Acinetobacter baumannii possesses high genetic plasticity and frequently acquires antimicrobial resistance genes. Here we investigated the role of natural transformation in these processes. Genomic DNA from different sources, including from carbapenem-resistant Klebsiella pneumoniae strains, was mixed with A. baumannii A118 cells. Selected transformants were analysed by whole-genome sequencing. In addition, bioinformatics analyses and in silico gene flow prediction were also performed to support the experimental results. Transformant strains included some that became resistant to carbapenems or changed their antimicrobial susceptibility profile. Foreign DNA acquisition was confirmed by whole-genome analysis. The acquired DNA most frequently identified corresponded to mobile genetic elements, antimicrobial resistance genes and operons involved in metabolism. Bioinformatics analyses and in silico gene flow prediction showed continued exchange of genetic material between A. baumannii and K. pneumoniae when they share the same habitat. Natural transformation plays an important role in the plasticity of A. baumannii and concomitantly in the emergence of multidrug-resistant strains.Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Place, Kori. California State University; Estados UnidosFil: Dotto, Cristian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Fernandez, Jennifer. California State University; Estados UnidosFil: Montaña, Sabrina Daiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Bahiense, Camila dos Santos. California State University; Estados UnidosFil: Soler Bistue, Alfonso J. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Iriarte, Andres. Universidad de la Republica. Facultad de Medicina; UruguayFil: Perez, Federico. Louis Stokes Cleveland Department Of Veterans Affairs; Estados UnidosFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Bonomo, Robert A.. Louis Stokes Cleveland Department Of Veterans Affairs; Estados UnidosFil: Melano, Roberto Gustavo. Public Health Ontario Laboratories; CanadáFil: Ramirez, Maria Soledad. California State University; Estados Unido

Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determine the Outcome?

Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.Fil: Pimentel, Camila. University of California; Estados UnidosFil: Le, Casin. University of California; Estados UnidosFil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados UnidosFil: Tolmasky, Marcelo E.. University of California; Estados UnidosFil: Ramirez, Maria Soledad. University of California; Estados Unido

Perfil epidemiológico de adolescentes vivendo com HIV/AIDS no Espírito Santo, Brasil: um estudo transversal

Introduction: The AIDS epidemic has undergone several transformations, and, in recent years, there has been an increase in cases of HIV/AIDS among adolescents and young people. Thus, it is essential to know this population to base health actions scientifically. Objective: This study aims to analyze the epidemiological profile of adolescents living with HIV/AIDS in Espírito Santo, Brazil. Methods: A descriptive, sectional study in which notifications of HIV/AIDS among adolescents aged 13 to 19 between 2010 and 2020 were analyzed. Results: 523 adolescents with HIV/AIDS were found in the analyzed period (an average of 47 cases/year). There was a predominance of male adolescents (68.8%), older than 16 years (mean=18.0 years), of mixed race/colour (54.6%), living in the metropolitan region near the capital. It was observed that the schooling of females is lower, with 47.2% of them in elementary school, while 45.0% of the boys are in high school. In most cases, the infection occurred via sexual intercourse, among men, through homosexual relations (55.0%), and among women, through heterosexual relations (82.2%). The HIV viral load was detectable in almost all (84.8%) cases, and 11 (6.8%) of these adolescents died. Conclusion: The epidemiological profile of HIV and AIDS cases among adolescents in Espírito Santo shows a higher frequency of cases in males aged 16 to 19 years, with incomplete high school education, who acquired HIV through unprotected sex in homosexual relationships. We highlight the high percentage of young people with detectable viral loads and deaths due to complications of AIDS.Introdução: A epidemia da AIDS passou por diversas transformações e, nos últimos anos, observa-se aumento de casos de HIV/AIDS entre adolescentes e jovens. Assim, é fundamental conhecer essa população para embasar cientificamente as ações em saúde. Objetivo: analisar o perfil epidemiológico de adolescentes que vivem com HIV/AIDS no Estado do Espírito Santo, Brasil. Método: estudo descritivo, seccional, no qual foram analisadas notificações de HIV/AIDS entre adolescentes de 13 a 19 anos, entre 2010 e 2020. Resultados: foram encontrados 523 adolescentes vivendo com HIV/AIDS no período analisado (média de 47 casos/ano). Prevaleceu os adolescentes do sexo masculino (68,8%), com mais de 16 anos (média=18,0 anos), de raça/cor parda (54,6%), residentes na região metropolitana, próxima a capital. Foi observado que a escolaridade do sexo feminino é menor, estando 47,2% delas no ensino fundamental, enquanto 45,0% dos rapazes já estão no ensino médio. Em grande parte dos casos a infecção ocorreu via sexual, sendo, entre os homens, através de relações homossexuais (55,0%) e entre as mulheres por meio de relações heterossexuais (82,2%). A carga viral de HIV foi detectável em quase totalidade (84,8%) dos casos e 11 (6,8%) destes adolescentes evoluíram para óbito. Conclusão: O perfil epidemiológico dos casos de HIV e AIDS, entre os adolescentes, no Estado do Espírito Santo, demonstra maior frequência de casos no sexo masculino, na faixa etária de 16 a 19 anos, com ensino médio incompleto, que adquiriram HIV por via sexual desprotegida, em relações homossexuais. Destaca-se a alta porcentagem de jovens com carga viral detectável e os óbitos em decorrência de complicações da AIDS

Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii

According to the Centers for Disease Control and Prevention, Acinetobacter baumannii is listed among the most threatening pathogens. A. baumannii is mainly a nosocomial pathogen with a distinctive ability to survive in multiple environments. These characteristics together with this bacterium’s ability to acquire antibiotic resistance determinants make it a notorious pathogen. The presence of human serum albumin (HSA) is associated with modifcation of expression levels in numerous genes. The presence of HSA in the culture medium is also correlated with a reduction in levels of the global suppressor histone-like nucleoid structure protein, H-NS. Comparative transcriptome analysis of the wild type and isogenic Δhns strains cultured in lysogeny broth (LB) in the presence or absence of HSA revealed that the expression of a subset of eleven genes are modifed in the Δhns cultured in LB and the wild-type strain in the presence of HSA, pointing out these genes as candidates to be regulated by the presence of HSA through H-NS. Six and fve of these genes were up- or down-regulated, respectively. Three of these genes have functions in quorum sensing (acdA, kar and fadD), one in quorum quenching (aidA), two in stress response (katE, ywrO), three in metabolism (phaC, yedL1, and yedL2), one in bioflm formation (csuAB), and one in β-oxidation of fatty acids (fadA). The regulation of these genes was assessed by: (i) transcriptional analysis and qPCR at the transcriptional level; and (ii) by determining the phenotypic characteristics of each function. The results of these studies support the hypothesis that HSA-mediated reduction of H-NS levels may be one very important regulatory circuit utilized by A. baumannii to adapt to selected environments, such as those where HSAcontaining human fuids are abundant.Fil: Escalante, Jenny. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Nishimura, Brent. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Pimentel, Camila. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Georgeos, Nardin. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Tomalsky, Marcelo E. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Ramírez, María Soledad. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular; Argentina.Fil: Tuttobene, Marisel Romina. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR); Argentina.Fil: Subils, Tomás. Instituto de Procesos Biotecnológicos y Químicos de Rosario (IPROBYQ-CONICET-UNR); Argentina.Fil: Sieira, Rodrigo. Fundación Instituto Leloir (IIBBA-CONICET); ArgentinaFil: Bonomo, Robert A. Research Service and GRECC. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; United States.Fil: Bonomo, Robert A. Case Western Reserve University School of Medicine. Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics. Departments of Medicine; United States.Fil: Bonomo, Robert A. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology ; United States

Effect of Serum Albumin, a Component of Human Pleural Fluid, on Transcriptional and Phenotypic Changes on Acinetobacter baumannii A118

Acinetobacter baumannii is a multidrug-resistant pathogen that causes numerous infections associated with high mortality rates. Exposure to human body fluids, such as human pleural fluid (HPF) and human serum, modulates gene expression in A. baumannii, leading to changes in its pathogenic behavior. Diverse degrees of effects at the transcriptional level were observed in susceptible and carbapenem-resistant strains. The transcriptional analysis of AB5075, a hyper-virulent and extensively drug-resistant strain showed changes in genes associated with quorum sensing, quorum quenching, fatty acids metabolism, and high-efficient iron uptake systems. In addition, the distinctive role of human serum albumin (HSA) as a critical component of HPF was evidenced. In the present work, we used model strain to analyze more deeply into the contribution of HSA in triggering A. baumannii’s response. By qRT-PCR analysis, changes in the expression level of genes associated with quorum sensing, biofilm formation, and phenylacetic acid pathway were observed. Phenotypic approaches confirmed the transcriptional response. HSA, a predominant component of HPF, can modulate the expression and behavior of genes not only in a hyper-virulent and extensively drug-resistant A. baumannii model, but also in other strains with a different degree of susceptibility and pathogenicity.Fil: Le, Casin. California State University; Estados UnidosFil: Pimentel, Camila. California State University; Estados UnidosFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Papp Wallace, Krisztina M.. Case Western Reserve University School of Medicine; Estados UnidosFil: Bonomo, Robert A.. Case Western Reserve University School of Medicine; Estados UnidosFil: Actis, Luis A.. Miami University; Estados UnidosFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Ramirez, Maria Soledad. California State University; Estados Unido

Involvement of the histone-like nucleoid structuring protein (H-ns) in acinetobacter baumannii’s natural transformation

Most Acinetobacter baumannii strains are naturally competent. Although some information is available about factors that enhance or reduce the frequency of the transformation of this bacterium, the regulatory elements and mechanisms are barely understood. In this article, we describe studies on the role of the histone-like nucleoid structuring protein, H-NS, in the regulation of the expression of genes related to natural competency and the ability to uptake foreign DNA. The expression levels of the natural transformation-related genes pilA, pilT, pilQ, comEA, comEC, comF, and drpA significantly increased in a ∆hns derivative of A. baumannii A118. The complementation of the mutant with a recombinant plasmid harboring hns restored the expression levels of six of these genes (pilT remained expressed at high levels) to those of the wild-type strain. The transformation frequency of the A. baumannii A118 ∆hns strain was significantly higher than that of the wild-type. Similar, albeit not identical, there were consequences when hns was deleted from the hypervirulent A. baumannii AB5075 strain. In the AB5075 complemented strain, the reduction in gene expression in a few cases was not so pronounced that it reached wild-type levels, and the expression of comEA was enhanced further. In conclusion, the expression of all seven transformation-related genes was enhanced after deleting hns in A. baumannii A118 and AB5075, and these modifications were accompanied by an increase in the cells’ transformability. The results highlight a role of H-NS in A. baumannii’s natural competence.Fil: Le, Casin. California State University; Estados UnidosFil: Pimentel, Camila. California State University; Estados UnidosFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Escalante, Jenny. California State University; Estados UnidosFil: Nishimura, Brent. California State University; Estados UnidosFil: Arriaga, Susana. California State University; Estados UnidosFil: Rodgers, Deja. California State University; Estados UnidosFil: Bonomo, Robert A.. Center for Antimicrobial Resistance and Epidemiology; Estados Unidos. Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados UnidosFil: Sieira, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Tolmasky, Marcelo E.. California State University; Estados UnidosFil: Ramírez, María Soledad. California State University; Estados Unido

Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii

In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections.Fil: Rodgers, Deja. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Le, Casin. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Pimentel, Camila. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular; Argentina.Fil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.Fil: Subils, Tomás. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos de Rosario (IPROBYQ-CONICET); Argentina.Fil: Escalante, Jenny. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Nishimura, Brent. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science. Center for Applied Biotechnology Studies; United States.Fil: Vescovi, Eleonora García. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.Fil: Sieira, Rodrigo. Fundación Instituto Leloir (IIBBA-CONICET); Argentina.Fil: Bonomo, Robert A. Louis Stokes Cleveland. Department of Veterans Affairs Medical Center. Research Service and GRECC; United States.Fil: Bonomo, Robert A. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics; United States.Fil: Bonomo, Robert A. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES); United States