Simultaneous Effect of Temperature and Irradiance on Growth and Okadaic Acid Production from the Marine Dinoflagellate Prorocentrum belizeanum

Benthic marine dioflagellate microalgae belonging to the genus Prorocentrum are a major source of okadaic acid (OA), OA analogues and polyketides. However, dinoflagellates produce these valuable toxins and bioactives in tiny quantities, and they grow slowly compared to other commercially used microalgae. This hinders evaluation in possible large-scale applications. The careful selection of producer species is therefore crucial for success in a hypothetical scale-up of culture, as are appropriate environmental conditions for optimal growth. A clone of the marine toxic dinoflagellate P. belizeanum was studied in vitro to evaluate its capacities to grow and produce OA as an indicator of general polyketide toxin production under the simultaneous influence of temperature (T) and irradiance (I0). Three temperatures and four irradiance levels were tested (18, 25 and 28 °C; 20, 40, 80 and 120 µE·m−2·s−1), and the response variables measured were concentration of cells, maximum photochemical yield of photosystem II (PSII), pigments and OA. Experiments were conducted in T-flasks, since their parallelepipedal geometry proved ideal to ensure optically thin cultures, which are essential for reliable modeling of growth-irradiance curves. The net maximum specific growth rate (µm) was 0.204 day−1 at 25 °C and 40 µE·m−2·s−1. Photo-inhibition was observed at I0 > 40 μEm−2s−1, leading to culture death at 120 µE·m−2·s−1 and 28 °C. Cells at I0 ≥ 80 µE·m−2·s−1 were photoinhibited irrespective of the temperature assayed. A mechanistic model for µm-I0 curves and another empirical model for relating µm-T satisfactorily interpreted the growth kinetics obtained. ANOVA for responses of PSII maximum photochemical yield and pigment profile has demonstrated that P. belizeanum is extremely light sensitive. The pool of photoprotective pigments (diadinoxanthin and dinoxanthin) and peridinin was not able to regulate the excessive light-absorption at high I0-T. OA synthesis in cells was decoupled from optimal growth conditions, as OA overproduction was observed at high temperatures and when both temperature and irradiance were low. T-flask culture observations were consistent with preliminary assays outdoors

El Uso de las Tecnologías Digitales como un Proceso Educativo en la Sociedad del Conocimiento.

La utilización de tecnologías digitales han transformado a nuestra sociedad, el modo de vida y la forma de pensar han cambiado y se ha transformado en una sociedad de conocimiento, debido a que todos los cambios han influido en la manera de presentar la información en los diferentes medios de comunicación a través de la web 2.0, las formas de aprendizaje como empezó el e-learnig, blended-learning y que posteriormente se trasladan a las aulas escolares, intentando optimizar y eficientizar los procesos de enseñanza, mediante el uso de las TICs. Es un reto para el sistema educativo desarrollar instrumentos que faciliten los procesos de enseñanza y comprensión en las aulas, ante un gran crecimiento de la cantidad y calidad de información que circula en todos los ámbitos de las áreas del conocimiento y la vida en la sociedad, ya que desde que aparece la Web se ha tenido una constante evolución en la vida diaria, como es hoy en día las redes sociales

Procedimiento para obtener extracto de pulpo, el producto obtenido y su aplicación como suplemento para el cultivo y criopreservación de tejido y de células en suspensión de invertebrados marinos

Número de publicación: ES2261054 A1 (01.11.2006) También publicado como: ES2261054 B1 (16.11.2007) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.)P200403171 (31.12.2004)Extracto de pulpo como suplemento para el cultivo y criopreservación de tejido y de células en suspensión de invertebrados marinos. La presente invención se refiere a un procedimiento para obtención de un suplemento para el cultivo in vitro y criopreservación de tejido y de células en suspensión de invertebrados marinos a base de extracto acuoso de pulpo homogeneizado y la utilización de dicho suplemento preparación de medios para la criopreservación de células en suspensión y de tejidos de organismos invertebrados marinos.Universidad de Almerí

Non-Hodgkin lymphomas of oral cavity

Non-Hodgkin lymphomas (NHL) often show up in an extranodal pattern, especially in the head and neck. Intraoral locations are much less frequent, particularly when they are single. This, in turn, can lead to a prolonged diagnosis and even to inadequate treatment. Different patients with initial extranodal location of NHL which were not previously diagnosed and in which it was manifested only intraoraly are presented in this paper. These cases are presented together with the additional examinations used for the early diagnosis and with the corresponding clinical pictures, as well as with the overview of other cases from the available literature

Longitudinal analysis about flourishing and its predictors during emerging adults from a gender perspective

Flourishing is defined as a combination of hedonic and eudaimonic well-being. The objective of this work was to analyze with a longitudinal design and from a gender perspective, which variables are related to flourishing in a sample of emerging adults. The sample was comprised of 400 emerging adults (67% women) who were interviewed at two different points in time: when they were 20.3 years old on average (DT=2.04), and three years later, at the average age of 23.7 (DT= 2,08). The variables studied were: perceived family income; resolution of core developmental tasks (having a job and living independently); The Inventory of the Dimensions of Emerging Adulthood; educational variables (academic commitment and perceived University performance) and social support (from friends, family, and partner). Results showed the differences in the variables which predict flourishing in males and females. Flourishing in males in Wave 2 is related to two W1 variables: the perception of emerging adulthood as an experimentation stage and the priority which youngsters give to their studies. For females, flourishing in W2 is related to two W1 variables: the consideration of this stage as a self-focused stage, and family support. These results vouch for the importance of analyzing gender differences throughout emerging adulthood. Results also highlight the role that educational context and social support provide to well-being during emerging adulthood. More research is needed from a gender perspective to explains the variables related to well-being throughout emerging adulthood, to generate evidence-based intervention programs that facilitate the transition into adult life

Analysis of a suppressive subtractive hybridization library of Alternaria alternata resistant to 2-propenyl isothiocyanate

AbstractBackgroundIsothiocyanates (ITCs) are natural products obtained from plants of the Brassicas family. They represent an environmentally friendly alternative for the control of phytopathogenic fungi. However, as it has been observed with synthetic fungicides, the possibility of inducing ITC-resistant strains is a major concern. It is, therefore, essential to understanding the molecular mechanisms of fungal resistance to ITCs. We analyzed a subtractive library containing 180 clones of an Alternaria alternata strain resistant to 2-propenyl ITC (2-pITC). After their sequencing, 141 expressed sequence tags (ESTs) were identified using the BlastX algorithm. The sequence assembly was carried out using CAP3 software; the functional annotation and metabolic pathways identification were performed using the Blast2GO program.ResultsThe bioinformatics analysis revealed 124 reads with similarities to proteins involved in transcriptional control, defense and stress pathways, cell wall integrity maintenance, detoxification, organization and cytoskeleton destabilization; exocytosis, transport, DNA damage control, ribosome maintenance, and RNA processing. In addition, transcripts corresponding to enzymes as oxidoreductases, transferases, hydrolases, lyases, and ligases, were detected. Degradation pathways for styrene, aminobenzoate, and toluene were induced, as well as the biosynthesis of phenylpropanoid and several types of N-glycan.ConclusionsThe fungal response showed that natural compounds could induce tolerance/resistance mechanisms in organisms in the same manner as synthetic chemical products. The response of A. alternata to the toxicity of 2-pITC is a sophisticated phenomenon including the induction of signaling cascades targeting a broad set of cellular processes. Whole-transcriptome approaches are needed to elucidate completely the fungal response to 2-pITC