Effect of pH and level of concentrate in the diet on the production of biohydrogenation intermediates in a dual-flow continuous culture

Milk fat depression in cows fed high-grain diets has been related to an increase in the concentration of trans-10 C-18:1 and trans-10, cis-12 conjugated linoleic acid (CLA) in milk. These fatty acids (FA) are produced as a result of the alteration in rumen biohydrogenation of dietary unsaturated FA. Because a reduction in ruminal pH is usually observed when high-concentrate diets are fed, the main cause that determines the alteration in the biohydrogenation pathways is not clear. The effect of pH (6.4 vs. 5.6) and dietary forage to concentrate ratios (F:C; 70:30 F:C vs. 30:70 F:C) on rumen microbial fermentation, effluent FA profile, and DNA concentration of bacteria involved in lipolysis and biohydrogenation processes were investigated in a continuous culture trial. The dual-flow continuous culture consisted of 2 periods of 8 d (5 d for adaptation and 3 d for sampling), with a 2 x 2 factorial arrangement of treatments. Samples from solid and liquid mixed effluents were taken for determination of total N, ammonia-N, and volatile fatty acid concentrations, and the remainder of the sample was lyophilized. Dry samples were analyzed for dry matter, ash, neutral and acid detergent fiber, FA, and purine contents. The pH 5.6 reduced organic matter and fiber digestibility, ammonia-N concentration and flow, and crude protein degradation, and increased nonammonia and dietary N flows. The pH 5.6 decreased the flow of C-18:0, trans-11 C-18:1 and cis-9, trans-11 CLA, and increased the flow of trans-10 C-18:1, C18:2n-6, C18:3n-3, trans-11, cis-15 C-18:2 and trans-10, cis-12 CLA in the 1 h after feeding effluent. The pH 5.6 reduced Anaerovibrio lipolytica (32.7 vs. 72.1 pg/10 ng of total DNA) and Butyrivibrio fibrisolvens vaccenic acid subgroup (588 vs. 1,394 pg/10 ng of total DNA) DNA concentrations. The high-concentrate diet increased organic matter and fiber digestibility, nonammonia and bacterial N flows, and reduced ammonia-N concentration and flow. The high-concentrate diet reduced trans-11 C-18:1 and trans-10 C-18:1, and increased C18:2n-6, C18:3n-3 and trans-10, cis-12 CLA proportions in the 1 h after feeding effluent. The increase observed in trans-10, cis-12 CLA proportion in the 1 h after feeding effluent due to the high-concentrate diet was smaller that that observed at pH 5.6. Results indicate that the pH is the main cause of the accumulation of trans-10 C-18:1 and trans-10, cis-12 CLA in the effluent, but the trans-10, cis-12 CLA proportion can be also affected by high levels of concentrate in the diet

Phase-Covariant Quantum Benchmarks

We give a quantum benchmark for teleportation and quantum storage experiments suited for pure and mixed test states. The benchmark is based on the average fidelity over a family of phase-covariant states and certifies that an experiment can not be emulated by a classical setup, i.e., by a measure-and-prepare scheme. We give an analytical solution for qubits, which shows important differences with standard state estimation approach, and compute the value of the benchmark for coherent and squeezed states, both pure and mixed.Comment: 4 pages, 2 figure

Desarrollo de un simulador de explotaciones lecheras para valorar el efecto de diferentes estrategias productivas sobre el rendimiento técnico y económico

publishedTomo I . Sección: Sistemas Ganaderos-Economía y Gestión. Sesión: Producción de leche. Ponencia nº 2

High-fidelity entanglement swapping with fully independent sources

Entanglement swapping allows to establish entanglement between independent particles that never interacted nor share any common past. This feature makes it an integral constituent of quantum repeaters. Here, we demonstrate entanglement swapping with time-synchronized independent sources with a fidelity high enough to violate a Clauser-Horne-Shimony-Holt inequality by more than four standard deviations. The fact that both entangled pairs are created by fully independent, only electronically connected sources ensures that this technique is suitable for future long-distance quantum communication experiments as well as for novel tests on the foundations of quantum physics.Comment: added technical details and extended introduction and conclusion, slightly modified the abstract, corrected a mistake in the affiliation

Conditional beam splitting attack on quantum key distribution

We present a novel attack on quantum key distribution based on the idea of adaptive absorption [calsam01]. The conditional beam splitting attack is shown to be much more efficient than the conventional beam spitting attack, achieving a performance similar to the, powerful but currently unfeasible, photon number splitting attack. The implementation of the conditional beam splitting attack, based solely on linear optical elements, is well within reach of current technology.Comment: Submitted to Phys. Rev.