Bacterial reduction of N-oxides of tobacco- specific nitrosamines (TSNA)

1 Contrary to established metabolic pattern, a recent investigation of NNK metabolism produced in rat urine higher levels of 4-(methylnitrosamino)-1-(3-pyridyl)-1- butanone (NNK) and 4-(methylnitrosamino)-1-(3-pyri dyl)-1-butanol (NNAL) than their N-oxides, suggesting that reconversion of N-oxides could occur after urine formation. 2 To verify the possible role of bacteria in the reduction of NNK-N-oxide and NNAL-N-oxide to their respective parent compounds, NNK and NNAL, in smokers with urinary tract infection (UTI), the N-oxides were isolated from the urine of rats treated with 5-3HNNK and individually incubated at 37°C with ten bacterial species in sterile human urine under different pH regimens. After incubation with the bacteria, aliquots of culture media were analyzed by high pressure liquid chromatography (HPLC) with radiochemical detection. 3 Escherichia coli, Enterobacter cloacae, Klebsiella pneumoniae and Proteus mirabilis possessed varying capacity to regenerate NNK and NNAL from their N- oxides while others showed no detectable reductive capability within 24 h. 4 This result constitutes the first experimental evidence that in tobacco users with concomitant UTI, bacterial regeneration of the procarcinogenic NNK and NNAL from their N-oxides could occur in the bladder leading to increased carcinogen burden in these individuals

Dynamic correlations of the Coulomb Luttinger liquid

The dynamic density response function, form-factor, and spectral function of a Luttinger liquid with Coulomb electron-electron interaction are studied with the emphasis on the short-range electron correlations. The Coulomb interaction changes dramatically the density response function as compared to the case of the short-ranged interaction. The form of the density response function is smoothing with time, and the oscillatory structure appears. However, the spectral functions remain qualitatively the same. The dynamic form-factor contains the $\delta$-peak in the long-wave region, corresponding to one-boson excitations. Besides, the multi-boson-excitations band exists in the wave-number region near to $2k_F$. The dynamic form-factor diverges at the edges of this band, while the dielectric function goes to zero there, which indicates the appearance of a soft mode. We develop a method to analyze the asymptotics of the spectral functions near to the edges of the multi-boson-excitations band.Comment: 11 pages, 3 figures, submitted to PR

Ladder approximation to spin velocities in quantum wires

The spin sector of charge-spin separated single mode quantum wires is studied, accounting for realistic microscopic electron-electron interactions. We utilize the ladder approximation (LA) to the interaction vertex and exploit thermodynamic relations to obtain spin velocities. Down to not too small carrier densities our results compare well with existing quantum Monte-Carlo (QMC) data. Analyzing second order diagrams we identify logarithmically divergent contributions as crucial which the LA includes but which are missed, for example, by the self-consistent Hartree-Fock approximation. Contrary to other approximations the LA yields a non-trivial spin conductance. Its considerably smaller computational effort compared to numerically exact methods, such as the QMC method, enables us to study overall dependences on interaction parameters. We identify the short distance part of the interaction to govern spin sector properties.Comment: 6 pages, 6 figures, to appear in Physical Review

Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51

HIV-1 integrase (IN) is the key enzyme catalyzing the proviral DNA integration step. Although the enzyme catalyzes the integration step accurately in vitro, whether IN is sufficient for in vivo integration and how it interacts with the cellular machinery remains unclear. We set up a yeast cellular integration system where integrase was expressed as the sole HIV-1 protein and targeted the chromosomes. In this simple eukaryotic model, integrase is necessary and sufficient for the insertion of a DNA containing viral LTRs into the genome, thereby allowing the study of the isolated integration step independently of other viral mechanisms. Furthermore, the yeast system was used to identify cellular mechanisms involved in the integration step and allowed us to show the role of homologous recombination systems. We demonstrated physical interactions between HIV-1 IN and RAD51 protein and showed that HIV-1 integrase activity could be inhibited both in the cell and in vitro by RAD51 protein. Our data allowed the identification of RAD51 as a novel in vitro IN cofactor able to down regulate the activity of this retroviral enzyme, thereby acting as a potential cellular restriction factor to HIV infection

Reliability of 2D ultrasound imaging associated with transient ShearWave Elastography method to analyze spastic gastrocnemius medialis muscle architecture and viscoelastic properties

PurposeThe aim of the study was to assess the reliability of pennation angle (PA) and muscle thickness (MT) 2D measurements and of shear elastic modulus measurement, using ultrasound imaging (US). Those measurements were made on spastic gastrocnemius medialis muscle at rest and at maximal passive stretching, in post-stroke hemiplegic patients. The paretic side measurements were compared to non-paretic side.Material and methodsFourteen patients took part in 2 inter-session reliability experiments, realized at a 7 days interval by the same operator. The Aixplorer® Supersonic US scanner with the transient ShearWave Elastography (SWE) software was used. The stretching experiments were made manually and controlled by a goniometer.ResultsThe reliability of the 2D measurements was good. The coefficient of variation (CV) was 6.30% for MT measurement at rest, 6.40% and 8.26% for PA at rest and at maximal passive stretching respectively. The reliability of the shear elastic modulus measurement in the sagittal plane was good only at rest with a CV of 9.86%, versus 40.58% at stretching. None of the shear elastic modulus measurements in the axial plane were good. At rest, MT and PA were weaker on the paretic side (14.25±3.12mm and 17.32±5.10°) versus non-paretic side (16.30±3.19mm and 21.08±5.05°) (P<0.0001 and P=0.006). At rest, there was a small difference in the shear elastic modulus between the paretic side and the non-paretic side (5.40±1.67kPa versus 6.20±2.18kPa, P=0.041).DiscussionThis is the first description of muscle spastic structure using SWE with Supersonic Shear Imaging. 2D US associated with SWE shows promise in terms of muscular atrophy quantification and muscle histological quality assessment. These structural properties reflect some of the functional abilities regardless of motor control. It should enable further research on therapies, which impact muscle tissue quality, such as botulinum neurotoxin injections

On the Ground State of Electron Gases at Negative Compressibility

Two- and three-dimensional electron gases with a uniform neutralizing background are studied at negative compressibility. Parametrized expressions for the dielectric function are used to access this strong-coupling regime, where the screened Coulomb potential becomes overall attractive for like charges. Closely examining these expressions reveals that the ground state with a periodic modulation of the charge density, albeit exponentially damped, replaces the homogeneous one at positive compressibility. The wavevector characterizing the new ground state depends on the density and is complex, having a positive imaginary part, as does the homogeneous ground state, and real part, as does the genuine charge density wave.Comment: 6 double-column pages, 2 figures. 2nd version is an extension of the 1st one, giving more detail

Le modele du PETTLEP ou comment optimiser l’efficacite de l’imagerie mentale

Le modèle du PETTLEP, modèle élaboré par deux chercheurs et psychologues du sport britanniques, nous offre une approche inhabituelle et novatrice de l’imagerie. Ce modèle permet d’améliorer la compréhension des processus impliqués dans l’imagerie mentale et d’optimiser ainsi son utilisation et son efficacité. Les grands principes de cette approche ont été et sont actuellement mis en application auprès des gymnastes féminines du pôle France INSEP

Energy-loss magnetic chiral dichroism (EMCD): Magnetic chiral dichroism in the electron microscope

A new technique called energy-loss magnetic chiral dichroism (EMCD) has recently been developed [P. Schattschneider, et al. Nature 441, 486 (2006)] to measure magnetic circular dichroism in the transmission electron microscope (TEM) with a spatial resolution of 10 nm. This novel technique is the TEM counterpart of x-ray magnetic circular dichroism, which is widely used for the characterization of magnetic materials with synchrotron radiation. In this paper we describe several experimental methods that can be used to measure the EMCD signal [P. Schattschneider, et al. Nature 441, 486 (2006); C. Hébert, et al. Ultramicroscopy 108(3), 277 (2008); B. Warot-Fonrose, et al. Ultramicroscopy 108(5), 393 (2008); L. Calmels, et al. Phys. Rev. B 76, 060409 (2007); P. van Aken, et al. Microsc. Microanal. 13(3), 426 (2007)] and give a review of the recent improvements of this new investigation tool. The dependence of the EMCD on several experimental conditions (such as thickness, relative orientation of beam and sample, collection and convergence angle) is investigated in the transition metals iron, cobalt, and nickel. Different scattering geometries are illustrated; their advantages and disadvantages are detailed, together with current limitations. The next realistic perspectives of this technique consist of measuring atomic specific magnetic moments, using suitable spin and orbital sum rules, [L. Calmels, et al. Phys. Rev. B 76, 060409 (2007); J. Rusz, et al. Phys. Rev. B 76, 060408 (2007)] with a resolution down to 2 to 3 n

Restricted and unrestricted Hartree-Fock calculations of conductance for a quantum point contact

Very short quantum wires (quantum contacts) exhibit a conductance structure at a value of conductance close to $0.7 \times 2e^2/h$. It is believed that the structure arises due to the electron-electron interaction, and it is also related to electron spin. However details of the mechanism of the structure are not quite clear. Previously we approached the problem within the restricted Hartree-Fock approximation. This calculation demonstrated a structure similar to that observed experimentally. In the present work we perform restricted and unrestricted Hartree-Fock calculations to analyze the validity of the approximations. We also consider dependence of the effect on the electron density in leads. The unrestricted Hartree-Fock method allows us to analyze trapping of the single electron within the contact. Such trapping would result in the Kondo model for the ``0.7 structure''. The present calculation confirms the spin-dependent bound state picture and does not confirm the Kondo model scenario.Comment: 6 pages, 9 figure