3D Microstructured Carbon Nanotube Electrodes for Trapping and Recording Electrogenic Cells

Electrogenic cells such as cardiomyocytes and neurons rely mainly on electrical signals for intercellular communication. Microelectrode arrays (MEAs) have been developed for long-term recording of cell signals and stimulation of electrogenic cells under low-cell-stress conditions, providing new insights in the behavior of electrogenic cells and the operation of the brain. To date, MEAs are relying on flat or needle-shaped electrode surfaces, mainly due to limitations in the lithographic processes. This paper relies on a previously reported elasto-capillary aggregation process to create 3D carbon nanotube (CNT) MEAs. This study shows that CNTs aggregate in well-shaped structures of similar size as cardiomyocytes are particularly interesting for MEA applications. This is because i) CNT microwells of the right diameter preferentially trap individual cardiomyocytes, which facilitates single cell recording without the need for clamping cells or signal deconvolution, and ii) once the cells are trapped inside of the CNT wells, this 3D CNT structure is used as an electrode surrounding the cell, which increases the cell-electrode contact area. As a result, this study finds that the recorded output voltages increase significantly (more than 200%). This fabrication process paves the way for future study of complex interactions between electrogenic cells and 3D recording electrodes.This work was supported by the Research Foundation—Flanders (FWO, Belgium) under Project No. 11S1214N. Michael De Volder was supported by the ERC Starting Grant (337739)—HIENA and the Marie Curie Grant CANA (618250). Davor Copic was supported by the Marie Curie Grant EmuCam (660351)

Influence of Temperature on Post-Breakage Behaviour of Laminated Glass Beams : Experimental Approach

The assessment of the post-breakage performances of laminated glass elementsused in construction need to take into account the sensitivity to the temperature ofthe mechanical behaviour and properties of the product, in particular of theinterlayer material. A general problem statement and an overview of differentexperimental approaches are firstly presented. Then results of specific orientationtests on pre-cracked laminated glass beams with a stiff interlayer of DuPont carriedat three different temperatures (23, 45 and 60°C) are presented and commented. Acomparison of the mechanical behaviour at the different temperatures is done,aiming to give a comprehensive order of magnitude of the sensitivity totemperature of the post-breakage behaviour observed during the tests

Enhanced membrane protein expression by engineering increased intracellular membrane production

Background: Membrane protein research is frequently hampered by the low natural abundance of these proteins in cells and typically relies on recombinant gene expression. Different expression systems, like mammalian cells, insect cells, bacteria and yeast are being used, but very few research efforts have been directed towards specific host cell customization for enhanced expression of membrane proteins. Here we show that by increasing the intracellular membrane production by interfering with a key enzymatic step of lipid synthesis, enhanced expression of membrane proteins in yeast is achieved. Results: We engineered the oleotrophic yeast, Yarrowia lipolytica, by deleting the phosphatidic acid phosphatase, PAH1, which led to massive proliferation of endoplasmic reticulum (ER) membranes. For all eight tested representatives of different integral membrane protein families, we obtained enhanced protein accumulation levels and in some cases enhanced proteolytic integrity in the Delta pah1 strain. We analysed the adenosine A2AR G-protein coupled receptor case in more detail and found that concomitant induction of the unfolded protein response in the Delta pah1 strain enhanced the specific ligand binding activity of the receptor. These data indicate an improved quality control mechanism for membrane proteins accumulating in yeast cells with proliferated ER. Conclusions: We conclude that redirecting the metabolic flux of fatty acids away from triacylglycerol-and sterylester-storage towards membrane phospholipid synthesis by PAH1 gene inactivation, provides a valuable approach to enhance eukaryotic membrane protein production. Complementary to this improvement in membrane protein quantity, UPR co-induction further enhances the quality of the membrane protein in terms of its proper folding and biological activity. Importantly, since these pathways are conserved in all eukaryotes, it will be of interest to investigate similar engineering approaches in other cell types of biotechnological interest, such as insect cells and mammalian cells

Performance of Sentry-Glas-laminated metal-reinforced glass beams at 23, -20, and 60 ºC

Keywords: 1=reinforced 2=beam 3=SentryGlas 4=interlayer 5=temperature 6=redundancy. Abstract To validate the novel concept of laminating a metal reinforcement to a glass beam using a SentryGlas (SG) interlayer, a series of bending tests has been performed at 23, -20 and 60°C on 1.5 m SG-laminated metal-reinforced glass beams. The beams consisted of three glass layers with a stainless steel box section laminated in between the glass at the lower edge. The test results showed high redundancy of the beam specimens at all tested temperature levels. However, the specific response of the beam specimens varied at different temperature levels. At 23°C the beam specimens showed the highest residual strength, whereas the residual strength was reduced at both -20 and 60°C. This difference in response was caused by a difference in glass cracking behaviour and the occurrence of plastic hinges in the beams. These plastic hinges limited the residual strength of the beam and probably originated from a more brittle response of the SG at -20°C and a lower metal-to-glass bond strength of the SG at 60°C. Since the SG-laminated metal-reinforced glass beams showed promising results, future research at TU Delft will further explore the possibilities of this concept

IL-4Rα Blockade by Dupilumab Decreases Staphylococcus aureus Colonization and Increases Microbial Diversity in Atopic Dermatitis.

Dupilumab is a fully human antibody to interleukin-4 receptor α that improves the signs and symptoms of moderate to severe atopic dermatitis (AD). To determine the effects of dupilumab on Staphylococcus aureus colonization and microbial diversity on the skin, bacterial DNA was analyzed from swabs collected from lesional and nonlesional skin in a double-blind, placebo-controlled study of 54 patients with moderate to severe AD randomized (1:1) and treated with either dupilumab (200 mg weekly) or placebo for 16 weeks. Microbial diversity and relative abundance of Staphylococcus were assessed by DNA sequencing of 16S ribosomal RNA, and absolute S. aureus abundance was measured by quantitative PCR. Before treatment, lesional skin had lower microbial diversity and higher overall abundance of S. aureus than nonlesional skin. During dupilumab treatment, microbial diversity increased and the abundance of S. aureus decreased. Pronounced changes were seen in nonlesional and lesional skin. Decreased S. aureus abundance during dupilumab treatment correlated with clinical improvement of AD and biomarkers of type 2 immunity. We conclude that clinical improvement of AD that is mediated by interleukin-4 receptor α inhibition and the subsequent suppression of type 2 inflammation is correlated with increased microbial diversity and reduced abundance of S. aureus

Performance evaluation of CHP with heat storage in buildings

Paper presented at the 5th International Conference on Heat Transfer, Fluid Mechanics and Thermodynamics, South Africa, 1-4 July, 2007.Combined heat and power (CHP) production gains more and more attention. Offices and public buildings often have a large thermal power demand in combination with a fairly large electrical power demand. On the other hand they are seldom occupied by night and in weekends, reducing the actual operational time of the heating system. This in turn brings down the financial benefits of investing in CHP. A second problem is that electrical and thermal demands are often shifted in time. The running time of the engine is again limited this way, as it is often not allowed to deliver electricity to the power grid. A possible solution is using heat storage. This way the CHP-engine can run when the electricity demand is high. In the paper a simulation model of CHP with gas engine and heat storage by means of a hot water vessel is developed. The model is validated through experiments on an engine and a vessel. This model is used to analyze the design, control and performance of cogeneration plants. It is shown that storage is marginal beneficial and the design has to be done with great care.cs201

Oximetry and Glucose Sensors A SMART SENSOR FOR BIOMEDICAL APPLICATIONS

Abstract Monolithic smart sensors for the voltammetric measurement of glucose or oxygen concentrations have been developed. Each smart sensor consists of a planar voltammetric sensor, a CMOS interface circuit and temperature sensor. The interface circuit and the temperature sensor are realised in a standard CMOS process. The sensor specific layers are added afterwards on the same chip with the CMOS compatible sensor technology developed for this purpose. Two versions of this smart sensor have been realised. The two electrode configuration with a Au working electrode has been implemented for the measurement of p02. The three electrode configuration with a Pt working electrode can measure glucose when an additional glucose oxidase membrane is applied on the electrodes. The interface circuit can apply voltages from +lV to -lV to the sensor and can handle sensor current ranges from 30 nA full scale to 1 pA full scale with a +/-2.5 V supply voltage. The temperature sensor has a sensitivity of 154 pV/K. The total dimensions of this smart sensor are 0.75 mm by 5 mm