Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction

All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences. In the past decade especially, driven by the progress in the field of massively parallel sequencing, numerous studies have comprehensively assessed the impact of particular manipulations on library complexity and quality, and characterized the activities and specificities of several key enzymes used in library construction. Fortunately, careful protocol design and reagent choice can substantially mitigate many of these biases, and enable reliable representation of sequences in libraries. This review aims to guide the reader through the vast expanse of literature on the subject to promote informed library generation, independent of the application

Post-breakage behaviour of laminated glass in structural applications

Firstly is introduced what the post-breakage behaviour of laminated safety glass is, and why it's important to can model it for designing structural glass elements. A general description of post-breakage behaviour and the different possible mechanisms leading to failure are presented. We then focus on the mechanical properties of the interlayer material, more specifically in the perspective to model its behaviour at large strain up to break. Typical results of standard uniaxial tensile tests on SGP samples (SentryGlas (R) Plus, interlayer of DuPont de Nemours) are shortly presented, and we then explain why those are insufficient to calibrate numeric material models to use in finite elements softwares. Finally perspectives for further experimental investigation with aiming to calibrate material models are presented

A story about standardization for design of glass works

This contribution attempts to give an overview of the European standardization framework which is related to the design and calculation of glass works in buildings. The different work levels, institutions, technical committees and workgroups, and the various types of documents and their statute will be introduced, explaining the difference between draft, experimental and final standards, between harmonized, support and design standards, the general and particular meaning of harmonization and implementation. The standardization framework is explained firstly from the point of view of European standardization policy and history, and secondly regarding the standardization framework in Belgium. In particular, an attempt is made to highlight some particularities existing in harmonization efforts of design methods and codes for glass works

The potential of glycomics as prognostic biomarkers in liver disease and liver transplantation

The study of glycomics is a novel and fascinating approach for the development of biomarkers. It has become clear that in the field of liver disease specific glycomic patters are present in specific disease states, which has led to the development of diagnostic biomarkers. In this manuscript, we will describe two new applications of this technology for the development of prognostic biomarkers. The first biomarker is associated with the risk of hepatocellular carcinoma development in patients with compensated cirrhosis. The second biomarker is present in perfusate and is related to the risk of primary non function occurrence after liver transplantation. The technology used for these biomarkers could easily be implemented on routine capillary electrophoresis equipment

Human T cell glycosylation and implications on immune therapy for cancer

Glycosylation is an important post-translational modification, giving rise to a diverse and abundant repertoire of glycans on the cell surface, collectively known as the glycome. When focusing on immunity, glycans are indispensable in virtually all signaling and cell-cell interactions. More specifically, glycans have been shown to regulate key pathophysiological steps within T cell biology such as T cell development, thymocyte selection, T cell activity and signaling as well as T cell differentiation and proliferation. They are of major importance in determining the interaction of human T cells with tumor cells. In this review, we will describe the role of glycosylation of human T cells in more depth, elaborate on the importance of glycosylation in the interaction of human T cells with tumor cells and discuss the potential of cancer immunotherapies that are based on manipulating the glycome functions at the tumor immune interface