A NURBS-based solid modeling to enhance rapid prototyping in the restoration of decorative elements

In this research, we describe a computer-aided approach to improve the reconstruction method of decorum in architectural surfaces and sculpture. The effects of withdrawal caused by catalysis of mold in silicone was evaluated and simulated by a NURBS-based solid modelling. A tolerance analysis model was developed to predict manufacturing precision levels. In particular, differential increment along three dimensions was performed considering different volume distributions. The methodology was validated by experimental data obtained during the coffered ceiling restoration of Teatro Massimo Vittorio Emanuele in Palermo. The proposed methodology allowed the reconstruction of decorations or fragments of decoration with high accuracy

Assessment of High Porosity Lattice Structures for Lightweight Applications

Additive manufacturing (AM) methods have a growing application in different fields such as aeronautical, automotive, biomedical, and there is a huge interest towards the extension of their use. In this paper, lattice structures for AM are analysed with regards to stiffness and printability in order to verify the suitability for applications where the main requirement of efficiency in terms of stiffness has to be balanced with other needs such as weight saving, ease of manufacturing and recycling of the material. At this aim, lattice structures with high porosity unit cells and large cell size made of a recyclable material were considered with a geometrical configuration allowing 3D printing without any supports. The lattice structures considered were based on body-centred cubic (BCC) and face centred cubic (FCC) unit cell combined with cubic cell. Finally, a multi-morphology lattice structure obtained by mixing different unit cells is also proposed. The lattice structures were modelled and structurally analysed by means of finite element method (FEM), manufactured with a Fusion deposition modelling (FDM) printer and evaluated in relation to printability and dimensional accuracy. The results show that the proposed structure with mixed cells is potentially advantageous in terms of weight saving in relation to the mechanical properties

COMPARING THE EFFECTS OF DIATHERMY AND DYNAMIC STRETCHING ON HIP FLEXOR FLEXIBILITY AND POSTURAL CONTROL

Context: Dynamic stretching has become a universally accepted practice used to increase flexibility, reduce muscle injury, and improve performance. In addition to stretching, deep heating modalities such as pulsed shortwave diathermy have been used to increase range of motion through deep heating. The premise for this study was to see if combining a dynamic stretching routine with a diathermy treatment would improve range of motion (ROM) and postural control. Purpose: The purpose of this repeated measures study was to assess the effectiveness of both pulsed shortwave diathermy and dynamic stretching on hip flexor range of motion and postural control. Participants: Three males and six females between the ages of 18 and 30 completed the study. All of these participants were moderately active and had not sustained an injury to their dominant leg six months prior to the study. Methods: Each participant completed all four trials (diathermy alone, dynamic stretching alone, diathermy, and dynamic stretching combined, and a control trial) over the course of a month. Active hip extension and the Y-Star Excursion Balance Test (Y-SEBT) were complete before and after each trial. Results: The 2x4 (time by trial) repeated measures ANOVA revealed no statistical significance for hip extension and Y-SEBT (p=0.817 and p= 0.367 respectively). Conclusion: This research intended to determine diathermy’s outcomes on increasing ROM and postural control in the hip flexors and how these effects could be enhanced by a dynamic stretching routine. All of the participants regardless of trial improved however, this was due to a practicing effect. Therefore the results of this study were found to be inconclusive for all trials performed

A novel mutation in isoform 3 of the plasma membrane Ca2+ pump impairs cellular Ca2+ homeostasis in a patient with cerebellar ataxia and laminin subunit 1\u3b1 mutations.

The particular importance of Ca2+ signaling to neurons demands its precise regulation within their cytoplasm. Isoform 3 of the plasma membrane Ca2+ ATPase (the PMCA3 pump), which is highly expressed in brain and cerebellum, plays an important role in the regulation of neuronal Ca2+. A genetic defect of the function of the PMCA3 pump has been described in one family with X-linked congenital cerebellar ataxia. Here we describe a novel mutation of the PMCA3 pump (ATP2B3) in a patient with global developmental delay, generalized hypotonia and cerebellar ataxia. The mutation (a R482H replacement) impairs the Ca2+ ejection function of the pump. It reduces the ability of the pump expressed in model cells to control Ca2+ transients generated by cell stimulation and impairs its Ca2+ extrusion function under conditions of low resting cytosolic Ca2+ as well. In silico analysis of the structural effect of the mutation suggests a reduced stabilization of the portion of the pump surrounding the mutated residue in the Ca2+-bound state. The patient also carries two missense mutations in LAMA1, encoding for laminin subunit 1\u3b1. On the basis of the family pedigree of the patient, the presence of both PMCA3 and LAMA1 mutations appears to be necessary for the development of the disease. Considering the observed defect in cellular Ca2+ homeostasis and the previous finding that PMCAs act as digenic modulators in Ca2+-linked pathologies, the PMCA3 dysfunction along with LAMA1 mutations could act synergistically to cause the neurological phenotype

PINK1/Parkin Mediated Mitophagy, Ca2+ Signalling, and ER-Mitochondria Contacts in Parkinson's Disease

Endoplasmic reticulum (ER)-mitochondria contact sites are critical structures for cellular function. They are implicated in a plethora of cellular processes, including Ca2+ signalling and mitophagy, the selective degradation of damaged mitochondria. Phosphatase and tensin homolog (PTEN)-induced kinase (PINK) and Parkin proteins, whose mutations are associated with familial forms of Parkinson's disease, are two of the best characterized mitophagy players. They accumulate at ER-mitochondria contact sites and modulate organelles crosstalk. Alterations in ER-mitochondria tethering are a common hallmark of many neurodegenerative diseases including Parkinson's disease. Here, we summarize the current knowledge on the involvement of PINK1 and Parkin at the ER-mitochondria contact sites and their role in the modulation of Ca2+ signalling and mitophagy

A new generation of bio-composite thermoplastic filaments for a more sustainable design of parts manufactured by FDM

The most recent developments of Fused Deposition Modelling (FDM) techniques are moving the application of Additive Manufacturing (AM) technologies toward new areas of investigation such as the biomedical, aerospace, and marine engineering in addition to the more consolidated industrial and civil fields. Some specific characteristics are required for the components designed for peculiar applications, such as complex geometries, lightweight, and high strength as well as breathability and aesthetic appearance specifically in the biomedical field. All these design specifications could be potentially satisfied by manufacturing with 3D printing techniques. Moreover, the development of purpose-dedicated filaments can be considered a key factor to successfully meet all the requirements. In this paper, fabrication and applications of five new thermoplastic materials with fillers are described and analyzed. They are organic bio-plastic compounds made of polylactic acid (PLA) and organic by-products. The growing interest in these new composite materials reinforced with organic by-products is due to the reduction of production management costs and their low environmental impact. In this study, the production workflow has been set up and described in detail. The main properties of these new thermoplastic materials have been analyzed with a major emphasis on strength, lightweight, and surface finish. The analysis showed that these materials can be particularly suitable for biomedical applications. Therefore, two different biomedical devices were selected and relative prototypes were manufactured with one of the analyzed thermoplastic materials. The feasibility, benefits, and performance of the thermoplastic material considered for these applications were successfully assessed

Modeling effects of crop production, energy development and conservation-grassland loss on avian habitat

Birds are essential components of most ecosystems and provide many services valued by society. However, many populations have undergone striking declines as their habitats have been lost or degraded by human activities. Terrestrial grasslands are vital habitat for birds in the North American Prairie Pothole Region (PPR), but grassland conversion and fragmentation from agriculture and energy-production activities have destroyed or degraded millions of hectares. Conservation grasslands can provide alternate habitat. In the United States, the Conservation Reserve Program (CRP) is the largest program maintaining conservation grasslands on agricultural lands, but conservation grasslands in the PPR have declined by over 1 million ha since the program’s zenith in 2007. We used an ecosystemservices model (InVEST) parameterized for the PPR to quantify grassland-bird habitat remaining in 2014 and to assess the degradation status of the remaining grassland-bird habitat as influenced by crop and energy (i.e., oil, natural gas, and wind) production. We compared our resultant habitat-quality ratings to grassland-bird abundance data from the North American Breeding Bird Survey to confirm that ratings were related to grassland-bird abundance. Of the grassland-bird habitat remaining in 2014, about 19% was degraded by crop production that occurred within 0.1 km of grassland habitats, whereas energy production degraded an additional 16%. We further quantified the changes in availability of grasslandbird habitat under various land-cover scenarios representing incremental losses (10%, 25%, 50%, 75%, and 100%) of CRP grasslands from 2014 levels. Our model identified 1 million ha (9%) of remaining grassland-bird habitat in the PPR that would be lost or degraded if all CRP conservation grasslands were returned to crop production. Grassland regions world-wide face similar challenges in maintaining avian habitat in the face of increasing commodity and energy production to sate the food and energy needs of a growing world population. Identifying ways to model the impacts of the tradeoff between food and energy production and wildlife production is an important step in creating solutions

Stochastic PCA-based bone models from inverse transform sampling: Proof of concept for mandibles and proximal femurs

Principal components analysis is a powerful technique which can be used to reduce data dimensionality. With reference to three-dimensional bone shape models, it can be used to generate an unlimited number of models, defined by thousands of nodes, from a limited (less than twenty) number of scalars. The full procedure has been here described in detail and tested. Two databases were used as input data: the first database comprised 40 mandibles, while the second one comprised 98 proximal femurs. The “average shape” and principal components that were required to cover at least 90% of the whole variance were identified for both bones, as well as the statistical distributions of the respective principal components weights. Fifteen principal components sufficed to describe the mandibular shape, while nine components sufficed to describe the proximal femur morphology. A routine has been set up to generate any number of mandible or proximal femur geometries, according to the actual statistical shape distributions. The set-up procedure can be generalized to any bone shape given a sufficiently large database of the respective 3D shapes

Efficiency Optimization in Medium Power Wind Turbines: an Innovative Mechanical Pitch Control System

The paper illustrates the design of a new mechanical system for propeller blades pitch calibration in medium power wind turbines. The peculiarity of this system is its capacity of adjusting through a feedback control system, which allows the wind turbine to capture the maximum amount of energy from the wind. In this work an axial drive system was studied by means of racks capable of linearly adjusting the pitch of all wind turbine propeller blades in an intrinsically synchronous way, with an advantage over the traditional methods of propeller blades pitch calibration. For different wind speeds the system adjusts the blades angle of incidence in order to reduce the rotation speed and keep the system as close as possible to the pre-established design conditions generating maximum energy with a high efficiency. The manuscript examines the main analyses and simulations conducted during the design phase. These show that the proposed method allows to reach higher efficiencies with a greater intrinsic stability compared to the traditional pitch control mechanisms in medium power wind turbines. The experimental results on the first prototypes confirm the efficiency increase