OSSERVATORIO SULLA DINAMICA ECONOMICO-FINANZIARIA DELLE IMPRESE DELLA MECCANICA SPECIALIZZATA IN PIEMONTE PRIMO RAPPORTO 1998-2001

Pubblicazione promossa dalla Regione Piemonte, Direzione Industria, Settore Osservatorio Settori Produttivi Industriali .

Nature and magnitude of wave loadings at Seawave Slot-cone Generators

The Seawave Slot-cone Generator (SSG) is a wave energy converter based on the overtopping principle,which has collected a good deal of funds in the last years, from both public and private investors.Although its functional response has been extensively researched, practically no tools exist for thestructural design. Based on the results of regular wave experiments conducted at the University ofNaplesFederico II(Italy), a number of design equations have been derived, which permit to estimate themagnitude of the wave pressures acting onto the outer face of the device, along with the respective risetimes. The reliability of the predictive methods have been then verified against the random waveexperiments ofVicinanza and Frigaard (2009)

Perfusion index variations in clinically and hemodynamically stable preterm newborns in the first week of life

<p>Abstract</p> <p>Background</p> <p>The perfusion index, derived from the pulse oximeter signal, seems to be an accurate predictor for high illness severity in newborns. The aim of this study was to determine the perfusion index values of clinically and hemodynamically stable preterm newborns in the first week of life.</p> <p>Methods</p> <p>Perfusion index recordings were performed on the first, third and seventh day of life on 30 preterm newborns. Their state of health was assessed according to clinical and behaviour evaluation and to the Score for Neonatal Acute Physiology.</p> <p>Results</p> <p>The median(interquartile range) perfusion index values were 0.9(0.6) on the first, 1.2(1.0) on the third, and 1.3(0.9) on the seventh day, with a significant increase between the first and the third day.</p> <p>Conclusions</p> <p>Perfusion index proved to be an easily applicable, non-invasive method for monitoring early postnatal changes in peripheral perfusion. Its trend during the first week of life suggests that its clinical application should take age into account. Further studies are needed to obtain reference perfusion index values from a larger sample of preterm newborns, to identify specific gestational age-related cut-off values for illness and to test the role of perfusion index in monitoring critically ill neonates.</p

PARP inhibitor ABT-888 affects response of MDA-MB-231 cells to doxorubicin treatment, targeting Snail expression

To overcome cancer cells resistance to pharmacological therapy, the development of new therapeutic approaches becomes urgent. For this purpose, the use of poly(ADP-ribose) polymerase (PARP) inhibitors in combination with other cytotoxic agents could represent an efficacious strategy. Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification that plays a well characterized role in the cellular decisions of life and death. Recent findings indicate that PARP-1 may control the expression of Snail, the master gene of epithelial-mesenchymal transition (EMT). Snail is highly represented in different resistant tumors, functioning as a factor regulating anti-apoptotic programmes. MDA-MB-231 is a Snail-expressing metastatic breast cancer cell line, which exhibits chemoresistance properties when treated with damaging agents. In this study, we show that the PARP inhibitor ABT-888 was capable to modulate the MDA-MB-231 cell response to doxorubicin, leading to an increase in the rate of apoptosis. Our further results indicate that PARP-1 controlled Snail expression at transcriptional level in cells exposed to doxorubicin. Given the increasing interest in the employment of PARP inhibitors as chemotherapeutic adjuvants, our in vitro results suggest that one of the mechanisms through which PARP inhibition can chemosensitize cancer cells in vivo, is targeting Snail expression thus promoting apoptosi

Transport Processes in Porous Media by Self-Potential Method

A controlled diffusion/infiltration column experimental activity was carried out with the aim of monitoring the leakage of a salty water plume by time-lapse self-potential (SP) measurements. In particular, three tracer tests with different NaCl concentrations (6.00, 1.00, and 0.25 g L−1) were performed and all the measured SP signals showed a sharp reduction corresponding to the arrival of saline front with negative electrical potential values (−78.99±3.24 mV, −54.52±2.28 mV, and −24.12±1.21 mV) which decrease with increasing volume of tracer introduced into the column. Then, measured self-potential values were converted into salt concentration ones by the Planck-Henderson equation and sand diffusion (D) and longitudinal dispersivity (αL) values were estimated by modelling the transport equations in the COMSOL Multiphysics environment. Finally, the results show that measured and estimated NaCl concentrations are well correlated

Aging and Parkinson's Disease: Inflammaging, neuroinflammation and biological remodeling as key factors in pathogenesis

In order to better understand the pathogenesis of Parkinson's Disease (PD) it is important to consider possible contributory factors inherent to the aging process, as age-related changes in a number of physiological systems (perhaps incurred within particular environments) appear to influence the onset and progression of neurodegenerative disorders. Accordingly, we posit that a principal mechanism underlying PD is inflammaging, i.e. the chronic inflammatory process characterized by an imbalance of pro- and anti-inflammatory mechanisms which has been recognized as operative in several age-related, and notably neurodegenerative diseases. Recent conceptualization suggests that inflammaging is part of the complex adaptive mechanisms (\ue2\u80\u9cre-modeling\ue2\u80\u9d) that are ongoing through the lifespan, and which function to prevent or mitigate endogenous processes of tissue disruption and degenerative change(s). The absence of an adequate anti-inflammatory response can fuel inflammaging, which propagates on both local (i.e.- from cell to cell) and systemic levels (e.g.- via exosomes and other molecules present in the blood). In general, this scenario is compatible with the hypothesis that inflammaging represents a hormetic or hormetic-like effect, in which low levels of inflammatory stress may prompt induction of anti-inflammatory mediators and mechanisms, while sustained pro-inflammatory stress incurs higher and more durable levels of inflammatory substances, which, in turn prompt a local-to-systemic effect and more diverse inflammatory response(s). Given this perspective, new treatments of PD may be envisioned that strategically are aimed at exerting hormetic effects to sustain anti-inflammatory responses, inclusive perhaps, of modulating the inflammatory influence of the gut microbiota

Landscape of Tumor Suppressor Mutations in Acute Myeloid Leukemia

Acute myeloid leukemia is mainly characterized by a complex and dynamic genomic instability. Next-generation sequencing has significantly improved the ability of diagnostic research to molecularly characterize and stratify patients. This detailed outcome allowed the discovery of new therapeutic targets and predictive biomarkers, which led to develop novel compounds (e.g., IDH 1 and 2 inhibitors), nowadays commonly used for the treatment of adult relapsed or refractory AML. In this review we summarize the most relevant mutations affecting tumor suppressor genes that contribute to the onset and progression of AML pathology. Epigenetic modifications (TET2, IDH1 and IDH2, DNMT3A, ASXL1, WT1, EZH2), DNA repair dysregulation (TP53, NPM1), cell cycle inhibition and deficiency in differentiation (NPM1, CEBPA, TP53 and GATA2) as a consequence of somatic mutations come out as key elements in acute myeloid leukemia and may contribute to relapse and resistance to therapies. Moreover, spliceosomal machinery mutations identified in the last years, even if in a small cohort of acute myeloid leukemia patients, suggested a new opportunity to exploit therapeutically. Targeting these cellular markers will be the main challenge in the near future in an attempt to eradicate leukemia stem cells

Experimental Investigation of the Self-Healing Potential of Bacteria for Sustainable Concrete Structures

Although concrete is the most widely used building material in the world, its limited tensile strength makes cracking a common phenomenon in concrete elements. This study investigates the potential of autonomous self-healing as an eco-friendly and lowcost method to increase the durability of concrete. The crack-healing potential of different types of high-alkaline-tolerant bacteria or calcite-precipitation microorganisms is investigated. High-alkaline-tolerant bacteria and calcite-precipitation microorganisms were used to retrofit lab-fractured concrete samples. The samples healed with each of these bacteria groups were cast and tested under compressive load up to failure to measure the compressive strength of the concrete samples. The outcomes of experimental tests on concrete samples healed with biological processes demonstrate how this technique can be implemented when retrofitting durability-enhanced, eco-friendly concrete structures to improve the strength of durability of the material and ultimately improve the durability of many forms of concrete infrastructure

The Wilms&apos; tumor (WT1) gene expression correlates with the International Prognostic Scoring System (IPSS) score in patients with myelofibrosis and it is a marker of response to therapy

The Wilms tumor gene WT1 is a useful marker of clonal hematopoiesis and it has been shown to be a good marker of residual disease and it reflects the response to therapy. Although myelofibrosis is characterized by mutations of JAK2 and calreticulin (CALR), these mutations are not useful to monitor response to therapy. In this study we demonstrated that in patients affected by myelofibrosis WT1 correlates with the International Prognostic Scoring System (IPSS) score at diagnosis. Furthermore WT1 is a good marker of response to JAK2 inhibitors especially for patients without blasts and for patients who develop anemia or thrombocytopenia not for progression but as therapy related toxicity. Finally, WT1 transcript reduction can mirror a benefit of therapy on the disease burden. This study demonstrated that WT1 is a good marker for monitoring the response to therapy in patients affected by myelofibrosis