Implementation of liquid culture for tuberculosis diagnosis in a remote setting: lessons learned.

Although sputum smear microscopy is the primary method for tuberculosis (TB) diagnosis in low-resource settings, it has low sensitivity. The World Health Organization recommends the use of liquid culture techniques for TB diagnosis and drug susceptibility testing in low- and middle-income countries. An evaluation of samples from southern Sudan found that culture was able to detect cases of active pulmonary TB and extra-pulmonary TB missed by conventional smear microscopy. However, the long delays involved in obtaining culture results meant that they were usually not clinically useful, and high rates of non-tuberculous mycobacteria isolation made interpretation of results difficult. Improvements in diagnostic capacity and rapid speciation facilities, either on-site or through a local reference laboratory, are crucial

Predicting Partisan Redistricting Disputes

Partisan redistricting disputes are relatively rare occurrences. This paper explores the factors that lead to partisan disputes over congressional redistricting plans. In previous work single party control of both houses of the state legislature and the governorship emerged as a key correlate of partisan redistricting in the 1980s. This paper presents an interactive statistical model of partisan redistricting plans. The basic conclusion is that in addition to single party control, the nature of the voting role and the political competitiveness of the states effect the likelihood of partisan redistricting

A comparison of strip and quadrat analyses of the woody plants on a central Indiana river bluff

This report is concerned with the distribution of woody plants on certain river hills in central Indiana. The location is in the vicinity of Blue Bluffs on White river in Morgan county. Most hills, and particularly river hills of a hundred or more feet in height, show certain obvious differences in the woody plants at the top and the bottom. The relative abundance and evenness of distribution of different species, however, is hard to ascertain by ordinary observation, hence various methods of sampling are used to determine more exactly what are the distributional relationships of various species. Strip and sample plot methods of timber estimation have long been in use by the foresters and lumbermen, and out of their methods have grown certain practices of the ecologist. In these studies there has been an effort to compare two methods of statistical analysis of vegetation, (1) sampling by line transects, and (2) sampling by quadrats, or regularly distributed squares

Differential Rearing Alters Forced Swim Test Behavior, Fluoxetine Efficacy, and Post-Test Weight Gain in Male Rats

Citation: Arndt, D. L., Peterson, C. J., & Cain, M. E. (2015). Differential Rearing Alters Forced Swim Test Behavior, Fluoxetine Efficacy, and Post-Test Weight Gain in Male Rats. Plos One, 10(7), 21. doi:10.1371/journal.pone.0131709Environmental factors play a key role in the etiology of depression. The rodent forced swim test (FST) is commonly used as a preclinical model of depression, with increases in escape-directed behavior reflecting antidepressant effects, and increases in immobility reflecting behavioral despair. Environmental enrichment leads to serotonergic alterations in rats, but it is unknown whether these alterations may influence the efficacy of common antidepressants. Male Sprague-Dawley rats were reared in enriched (EC), standard (SC), or isolated (IC) conditions. Following the rearing period, fluoxetine (10 or 20 mg/kg, i.p.) was administered 23.5 hrs, 5 hrs, and 1 hr before locomotor and FST measures. Following locomotor testing and FST exposure, rats were weighed to assess fluoxetine-, FST-, and environmental condition-induced moderations in weight gain. Results revealed an antidepressant effect of environmental enrichment and a depressant effect of isolation. Regardless of significant fluoxetine effects on locomotor activity, fluoxetine generally decreased swimming and increased immobility in all three environmental conditions, with IC-fluoxetine (10 mg/kg) rats and EC-fluoxetine (20 mg/kg) rats swimming less than vehicle counterparts. Subchronic 20 mg/kg fluoxetine also induced significant weight loss, and differential rearing appeared to moderate weight gain following FST stress. These results suggest that differential rearing has the ability to alter FST behaviors, fluoxetine efficacy, and post-stressor well-being. Moreover, 20 mg/kg fluoxetine, administered subchronically, may lead to atypical effects of those commonly observed in the FST, highlighting the importance and impact of both environmental condition and dosing regimen in common animal models of depression

In the Interests of clients or commerce? Legal aid, supply, demand, and 'ethical indeterminacy' in criminal defence work

As a professional, a lawyer's first duty is to serve the client's best interests, before simple monetary gain. In criminal defence work, this duty has been questioned in the debate about the causes of growth in legal aid spending: is it driven by lawyers (suppliers) inducing unnecessary demand for their services or are they merely responding to increased demand? Research reported here found clear evidence of a change in the handling of cases in response to new payment structures, though in ways unexpected by the policy's proponents. The paper develops the concept of 'ethical indeterminacy' as a way of understanding how defence lawyers seek to reconcile the interests of commerce and clients. Ethical indeterminacy suggests that where different courses of action could each be said to benefit the client, the lawyer will tend to advise the client to decide in the lawyer's own interests. Ethical indeterminacy is mediated by a range of competing conceptions of 'quality' and 'need'. The paper goes on to question the very distinction between 'supply' and 'demand' in the provision of legal services

A robust automated method to analyze rodent motion during fear conditioning

A central question in the study of LTP has been to determine what role it plays in memory formation and storage. One valuable form of learning for addressing this issue is associative fear conditioning. In this paradigm an animal learns to associate a tone and shock, such that subsequent presentation of a tone evokes a fear response (freezing behavior). Recent studies indicate that overlapping cellular processes underlie fear conditioning and LTP. The fear response has generally been scored manually which is both labor-intensive and subject to potential artifacts such as inconsistent or biased results. Here we describe a simple automated method that provides unbiased and rapid analysis of animal motion. We show that measured motion, in units termed significant motion pixels (SMPs), is both linear and robust over a wide range of animal speeds and detection thresholds and scores freezing in a quantitatively similar manner to trained human observers. By comparing the frequency distribution of motion during baseline periods and to the response to fox urine (which causes unconditioned fear), we suggest that freezing and non-freezing are distinct behaviors. Finally, we show how this algorithm can be applied to a fear conditioning paradigm yielding information on long and short-term associative memory as well as habituation. This automated analysis of fear conditioning will permit a more rapid and accurate assessment of the role of LTP in memory

Markov semigroups, monoids, and groups

A group is Markov if it admits a prefix-closed regular language of unique representatives with respect to some generating set, and strongly Markov if it admits such a language of unique minimal-length representatives over every generating set. This paper considers the natural generalizations of these concepts to semigroups and monoids. Two distinct potential generalizations to monoids are shown to be equivalent. Various interesting examples are presented, including an example of a non-Markov monoid that nevertheless admits a regular language of unique representatives over any generating set. It is shown that all finitely generated commutative semigroups are strongly Markov, but that finitely generated subsemigroups of virtually abelian or polycyclic groups need not be. Potential connections with word-hyperbolic semigroups are investigated. A study is made of the interaction of the classes of Markov and strongly Markov semigroups with direct products, free products, and finite-index subsemigroups and extensions. Several questions are posed.Comment: 40 pages; 3 figure