12 research outputs found
Larger and denser: an optimal design for surface grids of EMG electrodes to identify greater and more representative samples of motor units
The spinal motor neurons are the only neural cells whose individual activity can be non-invasively identified. This is usually done using grids of surface electromyographic (EMG) electrodes and source separation algorithms; an approach called EMG decomposition. In this study, we combined computational and experimental analyses to assess how the design parameters of grids of electrodes influence the number and the properties of the identified motor units. We first computed the percentage of motor units that could be theoretically discriminated within a pool of 200 simulated motor units when decomposing EMG signals recorded with grids of various sizes and interelectrode distances (IED). Increasing the density, the number of electrodes, and the size of the grids, increased the number of motor units that our decomposition algorithm could theoretically discriminate, i.e., up to 83.5% of the simulated pool (range across conditions: 30.5-83.5%). We then identified motor units from experimental EMG signals recorded in six participants with grids of various sizes (range: 2-36 cm2) and IED (range: 4-16 mm). The configuration with the largest number of electrodes and the shortest IED maximized the number of identified motor units (56±14; range: 39-79) and the percentage of early recruited motor units within these samples (29±14%). Finally, the number of identified motor units further increased with a prototyped grid of 256 electrodes and an IED of 2 mm. Taken together, our results showed that larger and denser surface grids of electrodes allow to identify a more representative pool of motor units than currently reported in experimental studies.Significance StatementThe application of source separation methods to multi-channel EMG signals recorded with grids of electrodes enables users to accurately identify the activity of individual motor units. However, the design parameters of these grids have never been discussed. They are usually arbitrarily fixed, often based on commercial availability. Here, we showed that using larger and denser grids of electrodes than conventionally proposed drastically increases the number of identified motor units. The samples of identified units are more balanced between early- and late-recruited motor units. Thus, these grids provide a more representative sampling of the active motor unit population. Gathering large datasets of motor units using large and dense grids will impact the study of motor control, neuromuscular modelling, and human-machine interfacing
Trypanosoma cruzi Gene Expression in Response to Gamma Radiation
Trypanosoma cruzi is an organism highly resistant to ionizing radiation. Following a dose of 500 Gy of gamma radiation, the fragmented genomic DNA is gradually reconstructed and the pattern of chromosomal bands is restored in less than 48 hours. Cell growth arrests after irradiation but, while DNA is completely fragmented, RNA maintains its integrity. In this work we compared the transcriptional profiles of irradiated and non-irradiated epimastigotes at different time points after irradiation using microarray. In total, 273 genes were differentially expressed; from these, 160 were up-regulated and 113 down-regulated. We found that genes with predicted functions are the most prevalent in the down-regulated gene category. Translation and protein metabolic processes, as well as generation of precursor of metabolites and energy pathways were affected. In contrast, the up-regulated category was mainly composed of obsolete sequences (which included some genes of the kinetoplast DNA), genes coding for hypothetical proteins, and Retrotransposon Hot Spot genes. Finally, the tyrosyl-DNA phosphodiesterase 1, a gene involved in double-strand DNA break repair process, was up-regulated. Our study demonstrated the peculiar response to ionizing radiation, raising questions about how this organism changes its gene expression to manage such a harmful stress
HIV-1 Vpu utilizes both cullin-RING ligase (CRL) dependent and independent mechanisms to downmodulate host proteins
Detection and localization of early diastolic forces within the left ventricle from inflow jet dynamics. A comparison between normal subjects and patients with dilated cardiomyopathy
Proanthocyanidin protects against acute zearalenone-induced testicular oxidative damage in male mice
RNA-binding proteins in bacteria.
RNA-binding proteins (RBPs) are central to most if not all cellular processes,
dictating the fate of virtually all RNA molecules in the cell. Starting with
pioneering work on ribosomal proteins, studies of bacterial RBPs have paved the
way for molecular studies of RNA-protein interactions. Work over the years has
identified major RBPs that act on cellular transcripts at the various stages of
bacterial gene expression and that enable their integration into
post-transcriptional networks that also comprise small non-coding RNAs. Bacterial
RBP research has now entered a new era in which RNA sequencing-based methods
permit mapping of RBP activity in a truly global manner in vivo. Moreover, the
soaring interest in understudied members of host-associated microbiota and
environmental communities is likely to unveil new RBPs and to greatly expand our
knowledge of RNA-protein interactions in bacteria