Municipal Wastewater Disinfection with Electromagnetic Waves using Escherichia coli Concentration as Measurement of Quantification

Wastewater treatment is essential to protecting the environment and human welfare. Although chlorination is widely used, the environmental and health concerns associated with chlorine are growing. Treatment facilities are implementing alternative technologies, though the cost and efficiency associated with these practices leave much room in the wastewater field for innovation. Hydropath Technologies Limited introduced a piece of equipment that uses the properties of a transformer to pass an alternating electric current through the pipe and into the contents of the channel. Hydroflow claims that the charged microorganisms react with the oppositely charged water molecule to force osmosis and kill the cell. Disinfection capabilities of three Hydroflow models with varying voltages are tested using municipal wastewater from the secondary clarifier using Escherichia coli concentration as the unit for quantification. After testing the results surrounding theses experiments cannot support the hypothesis that the Hydroflow technology could replace chlorination for municipal wastewater disinfection

High-Stakes Testing: A Study of the Relevance and Its Contribution to Career and College Readiness

Educators and policymakers have continued to search for the ideal medium for gauging learning and teacher accountability. After nearly 50 years since the inception of assessments to prove minimum competency, many attempts have been made to reform the process and the assessment tool. With the current focus in education being on career and college readiness and 21st century skills, the purpose of this study was to determine if high-stakes testing contributes to college and career readiness. College and career readiness and 21st century skills have been considered qualities needed to remain competitive in the global workplace. The most desirable qualities have been critical thinking skills, problem-solving skills, communication skills, and judgment and decision-making skills. College freshmen from a community college and a 4-year college were the participants of this mixed-methodology study. The sample was determined by students enrolled in English 111 or the equivalent, due to this being a requirement for all regardless of career path. Those participating were categorized by gender and the number of years since graduation from high school. Questions were presented through Likert-style surveys as well as interviews, that determined their experiences with high-stakes testing at their respective schools. Data collected were very similar when compared by years since graduation but changed significantly when compared by gender. Key findings included students’ desire to be assessed by a portfolio of project-based assignments rather than a single, multiple-choice exam. Myths about the negative connotation that follow high-stakes tests were debunked. Implications of the findings included student choice in the manner in which they are being assessed and the use of multiple projects for determining content mastery

Composite Elastic Skins for Shape-Changing Structures

Composite elastic skins having tailorable mechanical properties have been invented for covering shape-changing ( morphable ) structures. These skins are intended especially for use on advanced aircraft that change shapes in order to assume different aerodynamic properties. Many of the proposals for aircraft that could perform large aerodynamic shape changes require flexible skins that could follow shape changes of internal structures driven by actuators. Examples of such shape changes can include growth or shrinkage of bumps, conformal changes in wing planforms, cambers, twists, and bending of integrated leading- and trailing-edge flaps. Prior to this invention, there was no way of providing smooth aerodynamic surfaces capable of large deflections while maintaining smoothness and sufficient rigidity. Although latex rubber, silicone rubber, and similar conventional materials can be made into smooth coverings, they are not suitable for this purpose because, in order to impart required stiffness against out-of-plane bending, it would be necessary to make the coverings excessively thick, thereby necessitating the use of impractically large actuation forces. The basic idea of the invention is that of smoothly wrapping an underlying variable structure with a smooth skin that can be stretched or otherwise warped with low actuation force in one or both in-plane direction(s) and is relatively stiff against out-of-plane bending. It is envisioned that a skin according to the invention could be stretched as much as 20 percent in a desired direction. Because this basic idea admits of numerous variations, the following description is necessarily oversimplified for the sake of brevity

Flexible Volumetric Structure

A flexible volumetric structure has a first spring that defines a three-dimensional volume and includes a serpentine structure elongatable and compressible along a length thereof. A second spring is coupled to at least one outboard edge region of the first spring. The second spring is a sheet-like structure capable of elongation along an in-plane dimension thereof. The second spring is oriented such that its in-plane dimension is aligned with the length of the first spring's serpentine structure

Structure and Energetics of Ground-State Hypericin: Comparison of Experiment and Theory

Calculations of the energies of the ground-state un-ionized tautomers of hypericin have been performed at the RMP2/6-31G(d) level of theory, using geometries obtained with the 3-21G basis set and Hartree−Fock wave functions. It is found that only the so-called normal form is likely to be populated at room temperature and that only two of the three possible double tautomers correspond to minima on the potential energy surface. The effect of continuum aqueous solvation on the tautomer energies is negligible. The O---O distances between which the proton is transferred are reported and are consistent with that required for an adiabatic proton transfer, i.e., ∼2.5 Å. All 156 vibrational frequencies are tabulated and may be viewed at www.msg.ameslab.gov. For example, the vibrations in the range 320−660 cm-1 are coupled with O---O vibrations. The vibrations that are most clearly O---O vibrations occur in the range 400−500 cm-1. Twisting of the backbone occurs in a wide range of frequencies, from 230 to 1150 cm-1, while the motion corresponding to an inversion of the (nonplanar) backbone occur at very low frequencies, 80−150 cm-1. The results of these calculations are discussed in terms of ground-state heterogeneity of hypericin that has been invoked to explain its spectra and excited-state kinetics

NASA High-Reynolds Number Circulation Control Research - Overview of CFD and Planned Experiments

A new capability to test active flow control concepts and propulsion simulations at high Reynolds numbers in the National Transonic Facility at the NASA Langley Research Center is being developed. This technique is focused on the use of semi-span models due to their increased model size and relative ease of routing high-pressure air to the model. A new dual flow-path high-pressure air delivery station has been designed, along with a new high performance transonic sem -si pan wing model. The modular wind tunnel model is designed for testing circulation control concepts at both transonic cruise and low-speed high-lift conditions. The ability of the model to test other active flow control techniques will be highlighted. In addition, a new higher capacity semi-span force and moment wind tunnel balance has been completed and calibrated to enable testing at transonic conditions

Nursing Home Staff Palliative Care Knowledge and Practices: Results of a Large Survey of Frontline Workers

CONTEXT: Deficits in quality end-of-life care for nursing home (NH) residents are well known. Palliative care is promoted as an approach to improve quality. The Palliative Care Survey (PCS) is designed to measure NH staff palliative care knowledge and practice. OBJECTIVES: To comparing palliative care knowledge and practices across NH staff roles using the PCS, and to examine relationships between facility characteristics and PCS scores. METHODS: The PCS was administered to frontline NH staff-certified nursing assistants (CNAs), licensed practical nurses (LPNs), registered nurses (RNs), and social workers (SWs)-in 51 facilities in 2012. Descriptive statistics were calculated by job role. Linear mixed effects models were used to identify facility and individual factors associated with palliative care practice and knowledge. RESULTS: The analytic sample included 1200 surveys. CNAs had significantly lower practice and knowledge scores compared to LPNs, RNs, and SWs (P < 0.05). LPNs had significantly lower psychological, end-of-life, and total knowledge scores than RNs (P < 0.05 for all). Although knowledge about physical symptoms was uniformly high, end-of-life knowledge was notably low for all staff. A one-point higher facility star rating was significantly associated with a 0.06 increase in family communication score (P = 0.003; 95% CI: 0.02-0.09; SE = 0.02). Higher penetration of hospice in the NH was associated with higher end-of-life knowledge (P = 0.003; parameter estimate = 0.006; 95% CI: 0.002-0.010; SE = 0.002). Sixty-two percent of respondents stated that, with additional training, they would be interested in being leaders in palliative care. CONCLUSION: Given observed differences in palliative care practice and knowledge scores by staff training, it appears the PCS is a useful tool to assess NH staff. Low end-of-life knowledge scores represent an important target for quality improvement

Hospice in the nursing home: perspectives of front line nursing home staff

OBJECTIVE: Use of hospice has been associated with improved outcomes for nursing home residents and attitudes of nursing home staff toward hospice influences hospice referral. The objective of this study is to describe attitudes of certified nursing assistants (CNAs), nurses, and social workers toward hospice care in nursing homes. DESIGN, SETTING, AND PARTICIPANTS: We conducted a survey of 1859 staff from 52 Indiana nursing homes. MEASUREMENTS: Study data include responses to 6 scaled questions and 3 open-ended qualitative prompts. In addition, respondents who cared for a resident on hospice in the nursing home were asked how often hospice: (1) makes their job easier; (2) is responsive when a patient has symptoms or is actively dying; (3) makes care coordination smooth; (4) is needed; (5) taught them something; and (6) is appreciated by patients/families. Responses were dichotomized as always/often or sometimes/never. RESULTS: A total of 1229 surveys met criteria for inclusion. Of the respondents, 48% were CNAs, 49% were nurses, and 3% were social workers; 83% reported caring for a nursing home patient on hospice. The statement with the highest proportion of always/often rating was 'patient/family appreciate added care' (84%); the lowest was 'hospice makes my job easier' (54%). More social workers responded favorably regarding hospice responsiveness and coordination of care compared with CNAs (P = .03 and P = .05, respectively). CONCLUSIONS: A majority of staff responded favorably regarding hospice care in nursing homes. About one-third of nursing home staff rated coordination of care lower than other aspects, and many qualitative comments highlighted examples of when hospice was not responsive to patient needs, representing important opportunities for improvement

Caspase-dependent signaling underlies glioblastoma cell death in response to the fungal metabolite, fusarochromanone

Fungal metabolites continue to show promise as a viable class of anticancer agents. In the present study, we investigated the efficacy of the fungal metabolite, fusarochromanone (FC101), for its antitumor activities in glioblastomas, which have a median survival of less than two years and a poor clinical response to surgical resection, radiation therapy and chemotherapy. Using clinically applicable doses, we demonstrated that FC101 induced glioblastoma apoptotic cell death via caspase dependent signaling, as indicated by the cleavage of poly(ADP-ribose) polymerase, glioblastoma (PARP). FC101 also induced differential reactive oxygen species (ROS) levels in glioblastoma cells, contrasting a defined role of oxidative stress in apoptotic cell death observed with other fungal metabolites. Furthermore, the antitumorigenic effects of FC101 on tumor cell migration were assessed. Cell migration assays revealed that FC101 significantly reduced the migratory capacity of glioblastomas, which are incredibly invasive tumors. Taken together, the present study establishes FC101 as a candidate anticancer agent for the cooperative treatment of glioblastomas

Genetic characterization of Theileria equi infecting horses in North America: evidence for a limited source of U.S. introductions

Background: Theileria equi is a tick-borne apicomplexan hemoparasite that causes equine piroplasmosis. This parasite has a worldwide distribution but the United States was considered to be free of this disease until recently. Methods: We used samples from 37 horses to determine genetic relationships among North American T. equi using the 18S rRNA gene and microsatellites. We developed a DNA fingerprinting panel of 18 microsatellite markers using the first complete genome sequence of T. equi. Results: A maximum parsimony analysis of 18S rRNA sequences grouped the samples into two major clades. The first clade (n= 36) revealed a high degree of nucleotide similarity in U.S. T. equi, with just 0–2 single nucleotide polymorphisms (SNPs) among samples. The remaining sample fell into a second clade that was genetically divergent (48 SNPs) from the other U.S. samples. This sample was collected at the Texas border, but may have originated in Mexico. We genotyped T. equi from the U.S. using microsatellite markers and found a moderate amount of genetic diversity (2–8 alleles per locus). The field samples were mostly from a 2009 Texas outbreak (n= 22) although samples from five other states were also included in this study. Using Weir and Cockerham’s FST estimator (θ) we found strong population differentiation of the Texas and Georgia subpopulations (θ= 0.414), which was supported by a neighbor-joining tree created with predominant single haplotypes. Single-clone infections were found in 27 of the 37 samples (73%), allowing us to identify 15 unique genotypes. Conclusions: The placement of most T. equi into one monophyletic clade by 18S is suggestive of a limited source of introduction into the U.S. When applied to a broader cross section of worldwide samples, these molecular tools should improve source tracking of T. equi outbreaks and may help prevent the spread of this tick-borne parasite