Effect of age and nutrient restriction pre partum on beef suckler cow serum immunoglobulin concentrations, colostrum yield, composition and immunoglobulin concentration and immune status of their progeny

peer-reviewedThe effect of cow age (multiparous (MP) v. primiparous (PP)) and nutritional restriction pre partum (grass silage ad libitum v. straw only ad libitum for the last 15 (s.d. 3.3) days of gestation) on cow serum immunoglobulin (Ig) concentration, on colostrum yield, composition and Ig concentration and on calf serum Ig concentrations (at ~8 and 48 h post partum) using spring-calving Limousin Holstein-Friesian cows and their progeny was studied over 3 years. The method of colostrum administration (stomach tube vs. assisted suckling within 1 h post partum) on calf immune status was also investigated. When feeding colostrum the target was to give each calf 50 mL per kg birthweight via stomach tube. Colostrum yield and Ig concentration were measured following administration of oxytocin and hand-milking of half (Experiments 1 and 2) or the complete udder (Experiment 3). Following an 8-h period after birth during which suckling was prevented a further colostrum sample was obtained. There was no significant difference in first milking colostrum Ig subclass concentrations between the within-quarter fractions or between the front and rear quarters of the udder in either MP or PP cows. Colostrum Ig subclass concentrations at second milking were 0.46 to 0.65 of that at first milking. Compared to MP cows offered silage, colostrum yield and the mass of colostrum IgG1, IgG2, IgM, IgA and total Ig produced was lower (P < 0.001) for PP cows and the mass of IgG1, IgM and total Ig produced was lower (P < 0.05) for MP cows offered straw. Calves from PP cows and MP cows offered straw had significantly lower serum IgG1 and total Ig concentrations at 48 h post partum than calves from MP cows offered silage but there was no difference (P > 0.05) between colostrum feeding methods. In conclusion, calves from PP cows and MP cows offered straw had a lower humoral immune status than those from MP cows offered grass silage

Effect of suckler cow genotype on cow serum immunoglobulin (Ig) levels, colostrum yield, composition and Ig concentration and subsequent immune status of their progeny

peer-reviewedSurvival of the neonatal calf is largely dependent on humoral immunity. The objective of three experiments reported here was to compare cow serum immunoglobulin (Ig) concentration, colostrum yield, composition and Ig concentration and calf serum Ig concentrations at ~8- and 48-h post partum of spring-calving Charolais (C) and Beef × Holstein-Friesian (BF) cows and their progeny. Cows were individually offered a restricted allowance of grass silage pre partum in Experiments 1 and 2 and silage ad libitum in Experiment 3. In Experiment 1 calves were assisted to suckle after parturition. In Experiments 2 and 3, colostrum yield and Ig concentration were measured following administration of oxytocin and hand milking of half or the complete udder, respectively. It was intended to feed each calf 50 ml (Experiment 2) or 40 ml (Experiment 3) of colostrum per 1 kg birth weight via stomach tube. Following an 8-h period, during which suckling was prevented, a further colostrum sample was obtained. The decrease in cow serum IgG1 concentration pre partum was greater (P < 0.05) in BF cows than C cows. In comparison to BF cows, C cows had a lower colostrum yield (P < 0.001) and the colostrum had lower concentrations of dry matter (P < 0.01), crude protein (P < 0.05), fat (P < 0.05), IgG1 (P = 0.06), IgG2 (P < 0.01), IgM (P < 0.01) and Ig total (P < 0.05). The mass of IgG1, IgG2, IgM, IgA and Ig total in the colostrum produced was significantly lower for C cows than BF cows. Calves from C cows had significantly lower serum Ig subclass concentration at 48-h post partum than calves from BF cows. In conclusion, due to a lower Ig mass produced by their dams, calves from C cows had a lower humoral immune status than those from BF cowsTeagasc Walsh Fellowship Programm

Production and carcass traits of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais × Holstein-Friesian male cattle

peer-reviewedThe increased proportion of Holstein genetic material in the dairy herd has consequences for beef production in Ireland. A total of 72 spring-born male calves (24 Holsteins (HO), 24 Friesian (FR) and 24 Charolais × Holstein-Friesians (CH)) were reared from calfhood to slaughter. Calves were artificially reared indoors and spent their first summer at pasture following which they were assigned, on a breed basis, to a factorial combination of two production systems (intensive 19-month bull beef and extensive 25-month steer beef) and two slaughter weights (560 and 650 kg). After slaughter the pistola hind quarter was separated into fat, bone and muscle. Live-weight gain, carcass gain, kill-out proportion, carcass conformation and carcass fat scores were 830, 811 and 859 (s.e. 14.9) g/day, 540, 533, 585 (s.e. 7.7) g/day, 526, 538 and 561 (s.e. 3.0) g/kg, 1.51, 2.18 and 2.96 (s.e. 0.085), and 3.40, 4.25 and 4.06 (s.e. 0.104) for HO, FR and CH, respectively. Corresponding values for pistola weight as a proportion of carcass weight, pistola muscle proportion and pistola fat proportion were 458, 459 and 461 (s.e. 2.6) g/kg, 657, 645 and 667 (s.e. 3.7) g/kg, and 132, 161 and 145 (s.e. 4.1) g/kg. Compared with the intensive system, animals on the extensive system had a lower (P < 0.001) daily live-weight gain, kill-out proportion and a lower muscle proportion in the pistola. Increasing slaughter weight increased (P < 0.001) carcass weight and carcass fat score and reduced the proportion of muscle in the pistola. Allometric regression coefficients for pistola weight on side weight, and total bone, muscle and fat weights on pistola weight were 0.898, 0.755, 0.900 and 1.910 respectively. It is concluded that HO grew at least as fast as FR but had a lower killout proportion. Carcass conformation and fat scores were greater for FR than for HO and muscle proportion in the pistola was lower and total fat proportion was higher. Compared with FR, CH had heavier carcasses, a higher kill-out proportion and less fat and more muscle in the pistola

Body and carcass measurements, carcass conformation and tissue distribution of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais x Holstein-Friesian male cattle

peer-reviewedThe increased proportion of Holstein genes in the dairy herd may have undesirable consequences for beef production in Ireland. A total of 72 spring-born calves, (24 Holstein (HO), 24 Friesian (FR) and 24 Charolais X Holstein-Friesian (CH)) were reared from calfhood to slaughter. Calves were artificially reared indoors and spent their first summer at pasture following which they were assigned to a 3 breeds (HO, FR and CH) 2 production systems (intensive 19-month bull beef and extensive 25-month steer beef) 2 slaughter weights (560 and 650 kg) factorial experiment. Body measurements of all animals were recorded at the same time before the earliest slaughter date. After slaughter, carcasses were graded and measured and the pistola hind-quarter was separated into fat, bone and muscle. HO had significantly higher values for withers height, pelvic height and chest depth than FR, which in turn had higher values than CH. HO had a longer back and a narrower chest than either FR or CH, which were not significantly different. Carcass length and depth, pistola length, and leg length were 139.2, 134.4 and 132.0 (s.e. 0.81), 52.1, 51.3 and 47.7 (s.e. 0.38), 114.4, 109.0 and 107.0 (s.e. 0.65) and 76.7, 71.9 and 71.4 (s.e. 0.44) cm for HO, FR and CH, respectively. Breed differences in pistola tissue distribution between the joints were small and confined to the distal pelvic limb and ribs. There were relatively small breed differences in the distribution of pistola muscle weight between individual muscles. Body measurements were significantly greater for animals on the intensive system (bulls) than the extensive system (steers) in absolute terms, but the opposite was so when they were expressed relative to live weight. The only significant difference in relative carcass measurements between the production systems was for carcass depth, which was lower for the intensive compared with the extensive system. Increasing slaughter weight significantly increased all carcass measurements in absolute terms but reduced them relative to weight. It is concluded that there were large differences between the breed types in body and carcass measurements, and hence in carcass shape and compactness but differences in tissue distribution were small

Non-carcass parts and carcass composition of high dairy genetic merit Holstein, standard dairy genetic merit Friesian and Charolais × Holstein-Friesian steers

peer-reviewedThe increased use of Holstein genetic material in the dairy herd has consequences for beef production. A total of 24 spring-born calves comprising 8 Holsteins (HO), 8 Friesians (FR) and 8 Charolais × Holstein-Friesians (CH) were reared from calfhood to slaughter. At the end of the second grazing season they were assigned to a 3 (breeds; HO, FR and CH) × 2 (slaughter weights; 620 and 730 kg) factorial experiment and fin¬ished indoors. After slaughter carcasses were classified for conformation and fatness, all organs and non-carcass parts were weighed, and the right side of each carcass was dissected into fat, bone and muscle. Non-carcass parts, carcass weight, kill-out propor¬tion, carcass conformation score and m. longissimus area were 405, 398 and 368 (s.e. 8.31) g/kg empty body weight, 355, 344 and 383 (s.e. 9.4) kg, 509, 520 and 545 (s.e. 8.99) g/kg, 1.0, 2.0 and 3.1 (s.e. 0.16), 7616, 7096 and 9286 (s.e. 223.4) mm2 for HO, FR and CH, respectively. Corresponding proportions of carcass muscle and fat were 631, 614 and 656 (s.e. 8.4), and 165, 200 and 165 (s.e. 10.5) g/kg. Increasing slaughter weight increased the proportion of total non-carcass parts, carcass weight, carcass fat score and fat proportion, and reduced carcass muscle and bone proportions. It is concluded that differences in kill-out proportion between the two dairy breeds was primarily due to the lower proportion of gastrointestinal tract (GIT) in FR, and the higher kill-out proportion of CH was mainly due to lower proportions of GIT, internal organs and internal fat. In terms of beef production, HO and FR were broadly comparable for most traits except carcass conformation score and carcass fat proportion, which were lower for HO. CH was superior to the dairy breeds in all important production traits

High Cyanobacterial Abundance in Three Northeastern Gulf of Mexico Estuaries

Aquatic phytoplankton comprise a wide variety of taxa spanning more than 2 orders of magnitude in size, yet studies of estuarine phytoplankton often overlook the picoplankton, particularly chroococcoid cyanobacteria (cf. Synechococcus). Three Gulf of Mexico estuaries (Apalachicola Bay, FL; Pensacola Bay, FL; Weeks Bay, AL) were sampled during summer and fall 2001 to quantify cyanobacterial abundance, to examine how cyanobacterial abundance varied with hydrographic and nutrient distributions, and to estimate the contribution of cyanobacteria to the bulk phytoplankton community. Cyanobacterial abundances in all 3 estuaries were high, averaging 0.59 ± 0.76 X 109 L–1 in Apalachicola Bay, 1.7 ± 1.2 X 109 L–1 in Pensacola Bay and 2.4 ± 1.9 X 109 L–1 in Weeks Bay (mean ± standard deviation). Peak abundances typically occurred in the oligohaline zone (low salinity estuarine zone) during the summer. Freshwater sites had nearly undetectable abundances, and marine sites had abundances several-fold lower than the oligohaline zone. When converted to equivalent chlorophyll a concentrations, cyanobacteria comprised a large fraction of the total phytoplankton biomass, at times approaching 100% in all 3 systems. These observations clearly indicate a cyanobacterial community of estuarine origin that can make up a large proportion of phytoplankton biomass

A Teaching Case Using Cost/Benefit Relationships Within An ERP System

This paper describes a simulation model specifically for the Managerial Accounting Information Systems course—an SAP enabled course for junior Accounting majors.  Supporting and measuring the Supply Chain through technology is a key goal of Accounting and should therefore be reflected in an Accounting curriculum.  It will assist students to learn both the benefits, and costs, involved with implementing ERP software.  The purpose of this case is to present students with a set of business scenarios, and have the students make a knowledgeable decision using SAP R/3 software.  The scenarios will act as a simplified replication of the business environment; thus, they will provide the students with an integrated learning experience.  They will make business decisions to ensure the profitability cash flow of their respective product lines.  While making these decisions, students participate in the application and analysis provided by IDES.  IDES is a simulated company within SAP for demonstration and education purposes.  Eventually, the students will need to justify the existence of their division by evaluating benefits of the system verses its costs.  The model created further examines the cost/benefit relationship that is undertaken when an enterprise chooses to switch from a legacy system to an ERP implementation.  Students learn to compare the initial and recurring costs with the benefits derived through profitability and cash flow efficiencies.  In their analysis students are confronted with both structured and unstructured information allowing them to measure and assess a full range of strategic and tactical implementations.  To provide a “learning by doing” experience, the scenarios will be used to demonstrate the reporting functionality within IDES, the simulated company integrated into the SAP system.  Today’s global economy is putting a premium on the ability of students to evaluate a broad range of decisions in collaboration with adaptive supply chains.  A critical component of the evolving accounting curriculum lies in the ability to extend students beyond the numbers to evaluate the effective and efficient delivery of these supply chains

Comparative analysis between a low pathogenic and a high pathogenic influenza H5 hemagglutinin in cell entry

Avian influenza viruses continue to threaten globally with pandemic potential. The first step in a potential pandemic is the ability of the virus to enter human cells which is mediated by the viral surface glycoprotein hemagglutinin (HA). Viral entry of influenza is dependent upon the processing of the HA0 polypeptide precursor protein into HA1 and HA2 which is mediated by host cellular proteases. The sequence of the cleavage site which is recognized by host proteases has been linked with pathogenesis of various influenza viruses. Here we examined the effects of cleavage site sequences between a highly pathogenic H5N1 strain and a low pathogenic H5N2 strain to determine their effects on viral entry. From this analysis we determined that at the level of viral entry, the only observed difference between the low and high pathogenic strains is their ability to be cleaved by host cellular proteases

Intelligent subsystem interface for modular hardware system

A single chip application specific integrated circuit (ASIC) which provides a flexible, modular interface between a subsystem and a standard system bus. The ASIC includes a microcontroller/microprocessor, a serial interface for connection to the bus, and a variety of communications interface devices available for coupling to the subsystem. A three-bus architecture, utilizing arbitration, provides connectivity within the ASIC and between the ASIC and the subsystem. The communication interface devices include UART (serial), parallel, analog, and external device interface utilizing bus connections paired with device select signals. A low power (sleep) mode is provided as is a processor disable option

SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties.

BackgroundSerine proteases are important virulence factors for many pathogens. Recently, we discovered a group of trypsin-like serine proteases with domain organization unique to flatworm parasites and containing a thrombospondin type 1 repeat (TSR-1). These proteases are recognized as antigens during host infection and may prove useful as anthelminthic vaccines, however their molecular characteristics are under-studied. Here, we characterize the structural and proteolytic attributes of serine protease 2 (SmSP2) from Schistosoma mansoni, one of the major species responsible for the tropical infectious disease, schistosomiasis.Methodology/principal findingsSmSP2 comprises three domains: a histidine stretch, TSR-1 and a serine protease domain. The cleavage specificity of recombinant SmSP2 was determined using positional scanning and multiplex combinatorial libraries and the determinants of specificity were identified with 3D homology models, demonstrating a trypsin-like endopeptidase mode of action. SmSP2 displayed restricted proteolysis on protein substrates. It activated tissue plasminogen activator and plasminogen as key components of the fibrinolytic system, and released the vasoregulatory peptide, kinin, from kininogen. SmSP2 was detected in the surface tegument, esophageal glands and reproductive organs of the adult parasite by immunofluorescence microscopy, and in the excretory/secretory products by immunoblotting.Conclusions/significanceThe data suggest that SmSP2 is secreted, functions at the host-parasite interface and contributes to the survival of the parasite by manipulating host vasodilatation and fibrinolysis. SmSP2 may be, therefore, a potential target for anti-schistosomal therapy