480 research outputs found
Engineered nanofluidic platforms for single molecule detection, analysis and manipulation
Since the pioneering studies on single ion-channel recordings in 1976, single molecule
methods have evolved into powerful tools capable of probing biological systems with unprecedented
detail.
In this work, we build on the versatility of a type of nanofluidic devices, called nanopipettes,
to explore novel modes of single molecule detection and manipulation with the aim of improving
spatial and temporal control of biomolecules.
In particular, a novel nanopore configuration is presented, where biomolecules were
individually confined into a zeptoliter volume bridging two adjacent nanopores at the tip
of a nanopipette. As a result of this confinement, the transport of biomolecules such as
DNA and proteins was slow down by nearly three orders of magnitude, leading to an
improved sensitivity and superior signal-to-noise performances compared to conventional
nanopore sensing. Active ways of controlling the transport of biomolecule by combining
the advantages of nanopore single-molecule sensing and Field-Effect Transistors are also
presented. These hybrid platforms were fabricated in a simple two step process which
integrates a gold electrode at the apex of a nanopipette. We show that these devices were
effective in modulating the charge density of the nanopore and in actively switching "on"
and "off" the transport of DNA through the nanopore.
Finally, a nanoscale dielectrophoretic nanotweezer device has been developed for high
resolution manipulation and interrogation of individual entities. Two closely spaced carbon
nanoelectrodes were embedded at the apex of a nanopipette. Voltage and frequency applied
to the electrodes generated a highly localized force capable of trapping and manipulating a
broad range of biomolecules. These dielectrophoretic nanotweezers were suitable for probing
complex biological environments and a new technique for minimally invasive single-cell
nanobiopsy was established. Such study provides encouraging results on how nanopipettebased
platforms can be integrated as a future tool for routinely interrogating molecules at the
nanoscale.Open Acces
Does Sex Really Sell? Paradoxical Effects of Sexualization in Advertising on Product Attractiveness and Purchase Intentions
To test the “sex sells” assumption, we examined how Italian men and women react to sexualized advertising. Women showed
lower product attractiveness and purchase intentions toward products presented with sexualized female models than with neutral
ads, whereas men were unaffected by ads’ sexualization (Study 1, n = 251). Study 2 (n = 197) replicated the overall results. Study
3 (n = 198) tested hostile sexism as a moderator as well as negative emotions as a mediator of consumers’ responses. Especially
men with higher hostile sexism showed more purchase intentions after viewing female sexualized ads than neutral ads.Moreover,
women’s lower consumer responses toward sexualized female ads were due to higher negative emotions. Study 4 (n = 207)
included ads with both female and male models, replicating responses to female sexualization and showing that both women and
men had lower product attractiveness and purchase intentions toward male sexualized ads than neutral ads. Replicating and
extending Study 3’s results, women’s negative emotions was the mediator. The present study has practical implications for
marketers because it suggests that “sex does not sell.” In addition, considering both the psychological damage and practical
inefficacy of sexualized ads, our findings have important implications for public policy
Maistrus, picapedres e albaniles sardi nel tardogotico civile. viaggi, scambi di modelli e interpretazioni popolari
The master builders of Sardinia, in the time of the late Gothic, received the news from the Mediterranean regions from which important
masters came to build new architectures. Influenced by these innovations, Sardinian masters entered the circuit of cultural exchanges and
sometimes reworked, based on their previous training, solutions of different sign, evident in residential construction far from large urban centers,
as very evident lintels decorated according to local repertoires. Strengthened by this training, some Sardinian masters are documented
in important construction sites in the Catalan regions or in Sicily. Their skills concern the construction in raw earth or the construction of
large windows with lintel and arch, called sarde, documented in Sicily since 1350. The Sardinian masters, already the subject of attention in
the history of studies, can now be better interpreted as specialists rather than simple migrants looking for work. They probably were hired for
their innovative role anchored in archaic models
Conspiracy theories under the lens of social psychology
openIl cospirazionismo inteso come aderire a una teoria della cospirazione al fine di spiegare gli eventi sociali è un fenomeno che non riguarda solo la nostra epoca, infatti esiste già dall’antichità , ma è diventato particolarmente rilevante in questo momento storico, dove eventi di crisi come la pandemia di Covid-19 mettono in dubbio le certezze fornite dal metodo scientifico e dalle istituzioni. Vari studi negli anni si sono occupati di spiegare le cause individuali e sociali della formazione e della credibilità delle teorie del complotto riguardo i temi più disparati ma si occupano anche di riportare nel complesso le conseguenze sociali e politiche delle teorie della cospirazione, e delle possibili soluzioni al fine di comprendere come gestire il fenomeno
- …