Activity Of Arrabidaea Chica (humb. & Bonpl.) Verlot Extracts Obtained By Biotechnological Processes On Fibroblast And Human Tumor Cells [atividade De Extratos De Arrabidaea Chica (humb. & Bonpl.) Verlot Obtidos Por Processos BiotecnolÓgicos Sobre A ProliferaÇÃo De Fibroblastos E CÉlulas Tumorais Humanas]

Arrabidaea chica (H&B) Verlot is a plant popularly known as Pariri and this species is a known source of anthocyanins, flavonoids and tannins. This report describes an approach involving enzymatic treatment prior to extraction procedures to enhance A chica crude extract anticancer activity. Anticancer activity in human cancer cell lines in vitro using a 48 h SRB cell viability assay was performed to determine growth inhibition and cytotoxic properties. The final extraction yield without enzyme treatment was higher (24.28%) compared to the enzyme-treated material (19.03%), with an enhanced aglycones anthocyanin ratio as determined by HPLC- DAD and LC-MS with direct infusion.363431436Chapman, E., Perkin, A.G., Robinson, R., (1927) J. Chem. Soc, 49, p. 3015Corrêa, M.P., (1984) Dicionário das Plantas Úteis Do Brasil e das Exóticas Cultivadas, , Re- edição Imprensa Nacional: Rio de JaneiroLorenzi, H., Matos, F.J.A., (2002) Plantas Medicinais No Brasil Nativas e Exóticas, , 1a ed., Instituto Platarum: São PauloJorge, M.P., Madjarof, C., Ruiz, A.L.T.G., Fernandes, A.T., Rodrigues, R.A.F., Sousa, I.M.O., Foglio, M.A., Carvalho, J.E., (2008) J. Ethnopharmacol, 118, p. 361Barbosa, W.L.R., Nascimento Pinto, L., Quignard, E., Vieira, J.M.S., Silva Jr., J.O.C., Albuquerque, S., (2010) Rev. Bras. Farmacogn, 18, p. 544Höfling, J.F., Anibal, P.C., Obando-Pereda, G.A., Peixoto, I.A.T., Furletti, V.F., Foglio, M.A., Gonçalves, R.B., (2010) Braz. J. Biol, 70, p. 1065Castaneda-Ovando, A., Pacheco-Hernandez, M.L., Paez-Hernandez, M.E., Rodriguez, J.A., Galan-Vidal, C.A., (2009) Food Chem, 113, p. 859Scogin, R., (1927) Biochem. Syst. Ecol, 8, p. 273Harborne, J.B., (1967) Phytochemistry, 6, p. 1643Harborne, J.B., Williams, C.A., (2001) Nat. Prod. Rep, 18, p. 310Takemura, O.S., Iinuma, M., Tosa, H., Miguel, O.G., Moreira, E.A., Nozawa, Y., (1995) Phytochemistry, 38, p. 1299Zorn, B., García-Pineres, A.J., Castro, V., Murillo, R., Mora, G., Merfort, I., (2001) Phytochemistry, 56, p. 831Devia, B., Llabres, G., Wouters, J., Dupont, L., Escribano-Bailon, M.T., De Pascual-Teresa, S., Angenot, L., Tits, M., (2002) Phytochem. Anal, 13, p. 63Alcerito, T., Barbo, F.E., Negri, G., Santos, D.Y.A.C., Meda, C.I., Young, M.C.M., Chávez, D., Blatt, C.T.T., (2002) Biochem. Syst. Ecol, 30, p. 677Pauletti, P.M., Castro-Gamboa, I., Silva, D.H.S., Young, M.C.M., Tomazela, D.M., Eberlin, M.N., Bolzani, V.S., (2003) J. Nat.Prod, 66, p. 1384Março, P.H., Poppi, R.J., Scarminio, I.S., (2008) Quim. Nova, 31, p. 1218Poorna, C.A., Prema, P., (2006) Biochem. Eng. J., 32, p. 106Kim, D.H., Kim, J.H., Bae, S.I., Seo, J.H., Oh, T.K., Lee, C.H., (2005) J. Agric. Food Chem, 53, p. 2541Li, H.-B., Wong, C.-C., Cheng, K.-W., Chen, F., (2008) LWT - Food Sci. Technol, 2, p. 104Brasil, Agência Nacional de Vigilância Sanitária (ANVISA)Resolução RE No 899, de 29/05/2003. Diário Oficial da União de 30/05/2003Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Boyd, M., (1991) J. Nat. Cancer Inst, 83, p. 757Shoemaker, R.H., (2006) Nat. Rev. Cancer, 6, p. 813Figueira, G.M., Ramelo, P.R., Ogasawara, D.C., Montanari, I., Zucchi, M.I., Cavallari, M.M., Foglio, M.A., (2010) Am. J. Bot, 98, p. 283Duarte, M.C.T., Pellegrino, A.C.A., Portugal, E.P., Ponezi, A.N., Franco, T.T., (2001) Braz. J. Microbiol, 31, p. 90Taffarello, D.Dissertação de Mestrado, Universidade de São Paulo, Brasil, 2008Zafra-Stone, S., Yasmin, T., Bagchi, M., Chatterjee, A., Vinson, J.A., Bagchi, D., (2007) Mol. Nutr. Food Res, 51, p. 675Kong, J.M., Chia, L.S., Goh, N.-K., Chia, T.-F., Brouillard, R., (2003) Phytochemistry, 64, p. 923Fouche, G., Cragg, G.M., Pillay, P., Kolesnikova, N., Maharaj, V.J., Senabe, J., (2008) J. Ethnopharmacol, 119, p. 455Cai, Y.-Z., Xing, J., Sun, M., Zhan, Z.-Q., Corke, H., (2005) J. Agric. Food Chem, 53, p. 994