Diversidade genética em populações de Sclerotinia sclerotiorum na cultura de feijoeiro (Phaseolus vulgaris L.).

Este trabalho teve como objetivo determinar a estrutura genética de três populações de campo de S. sclerotiorum obtidas nos Estados de Goiás, Minas Gerais e Mato Grosso

Substratos e planta matriz na sobrevivência e crescimento de mudas de cambará.

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Constatação de Curvularia eragrostides em mudas de dendÛ no Estado do Pará.

Resumos do XXXVIII Congresso Brasileiro de Fitopatologia. Resumo 207. Título: dendÚ [i.e. dendê]

Relação entre polimorfismos no gene da leptina, ganho de peso e concentração sérica de leptina em bovinos de corte.

O objetivo do trabalho é estabelecer a relação de polimorfismos no gene da leptina com a quantidade de leptina sérica e ganho de peso durante o confinamento, visando a utilização de dados obtidos em programas de seleção animal. Os bovinos confinados utilizados na pesquisa serão provenientes da Fazenda Tuju Puitan. Serão objetos da pesquisa 100 animais com idade média de 12 meses

Mentha rotundifolia: um novo hospedeiro de Thanatephorus cucumeris no estado do Pará.

Resumos do XXXVIII Congresso Brasileiro de Fitopatologia. Resumo 091

Kinetics of b-galactosidase immobilized on polysiloxanepolyvinyl alcohol magnetic composite – POS-PVAM

b-Galactosidase is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes. In this work, b-Galactosidase was covalently immobilized onto a POS-PVAM using glutaraldehyde as activating agent and its hydrolytic properties evaluated. For both soluble and immobilized b-Galactosidase, the optimal temperature and pH were found to be 50 ºC and 6.5, respectively. The immobilized enzyme showed to be more resistant than the soluble form when hydrolysis experiments were performed out within the above optimal condition, being the observed difference in activity more pronounced for temperatures higher than 50ºC. An enhancement of the thermal stability of the immobilized enzyme was also observed. The apparent Km and Ea for both soluble (7.377 ± 1.303mM and 25.51 ± 8.72Kj mol-1) and immobilized enzyme (7.841 ± 1.189mM and 32.61 ± 5.82Kj mol-1) showed to be not significantly different. The immobilization also proved to be advantageous as, after twenty reutilizations, the immobilized enzyme retained about 52% of its initial activity. These results clearly demonstrate that POS-PVAM may be used for b- Galactosidase immobilization since, besides improving the enzyme hydrolytic properties, its separation from the obtained reaction products is easier to accomplish

The Novel P.e89k Mutation In The Sry Gene Inhibits Dna Binding And Causes The 46,xy Disorder Of Sex Development.

Male sex determination in humans is controlled by the SRY gene, which encodes a transcriptional regulator containing a conserved high mobility group box domain (HMG-box) required for DNA binding. Mutations in the SRY HMG-box affect protein function, causing sex reversal phenotypes. In the present study, we describe a 19-year-old female presenting 46,XY karyotype with hypogonadism and primary amenorrhea that led to the diagnosis of 46,XY complete gonadal dysgenesis. The novel p.E89K missense mutation in the SRY HMG-box was identified as a de novo mutation. Electrophoretic mobility shift assays showed that p.E89K almost completely abolished SRY DNA-binding activity, suggesting that it is the cause of SRY function impairment. In addition, we report the occurrence of the p.G95R mutation in a 46,XY female with complete gonadal dysgenesis. According to the three-dimensional structure of the human SRY HMG-box, the substitution of the conserved glutamic acid residue by the basic lysine at position 89 introduces an extra positive charge adjacent to and between the positively charged residues R86 and K92, important for stabilizing the HMG-box helix 2 with DNA. Thus, we propose that an electrostatic repulsion caused by the proximity of these positive charges could destabilize the tip of helix 2, abrogating DNA interaction.44361-