Relationship of cognitive function in patients with schizophrenia in remission to disability: a cross-sectional study in an Indian sample

Background: Cognitive deficits in various domains have been consistently replicated in patients with schizophrenia. Most studies looking at the relationship between cognitive dysfunction and functional disability are from developed countries. Studies from developing countries are few. The purpose of the present study was to compare the neurocognitive function in patients with schizophrenia who were in remission with that of normal controls and to determine if there is a relationship between measures of cognition and functional disability. <p/>Methods: This study was conducted in the Psychiatric Unit of a General Hospital in Mumbai, India. Cognitive function in 25 patients with schizophrenia in remission was compared to 25 normal controls. Remission was confirmed using the brief psychiatric rating scale (BPRS) and scale for the assessment of negative symptoms (SANS). Subjects were administered a battery of cognitive tests covering aspects of memory, executive function and attention. The results obtained were compared between the groups. Correlation analysis was used to look for relationship between illness factors, cognitive function and disability measured using the Indian disability evaluation and assessment scale. <p/>Results: Patients with schizophrenia showed significant deficits on tests of attention, concentration, verbal and visual memory and tests of frontal lobe/executive function. They fared worse on almost all the tests administered compared to normal controls. No relationship was found between age, duration of illness, number of years of education and cognitive function. In addition, we did not find a statistically significant relationship between cognitive function and scores on the disability scale. <p/>Conclusion: The data suggests that persistent cognitive deficits are seen in patients with schizophrenia under remission. The cognitive deficits were not associated with symptomatology and functional disability. It is possible that various factors such as employment and family support reduce disability due to schizophrenia in developing countries like India. Further studies from developing countries are required to explore the relationship between cognitive deficits, functional outcome and the role of socio-cultural variables as protective factors

Oxidative metabolism and PGC-1beta attenuate macrophage-mediated inflammation.

Complex interplay between T helper (Th) cells and macrophages contributes to the formation and progression of atherosclerotic plaques. While Th1 cytokines promote inflammatory activation of lesion macrophages, Th2 cytokines attenuate macrophage-mediated inflammation and enhance their repair functions. In spite of its biologic importance, the biochemical and molecular basis of how Th2 cytokines promote maturation of anti-inflammatory macrophages is not understood. We show here that in response to interleukin-4 (IL-4), signal transducer and activator of transcription 6 (STAT6) and PPARgamma-coactivator-1beta (PGC-1beta) induce macrophage programs for fatty acid oxidation and mitochondrial biogenesis. Transgenic expression of PGC-1beta primes macrophages for alternative activation and strongly inhibits proinflammatory cytokine production, whereas inhibition of oxidative metabolism or RNAi-mediated knockdown of PGC-1beta attenuates this immune response. These data elucidate a molecular pathway that directly links mitochondrial oxidative metabolism to the anti-inflammatory program of macrophage activation, suggesting a potential role for metabolic therapies in treating atherogenic inflammation

Membrane Insertion for the Detection of Lipopolysaccharides: Exploring the Dynamics of Amphiphile-in-Lipid Assays

<div><p>Shiga toxin-producing <i>Escherichia coli</i> is an important cause of foodborne illness, with cases attributable to beef, fresh produce and other sources. Many serotypes of the pathogen cause disease, and differentiating one serotype from another requires specific identification of the O antigen located on the lipopolysaccharide (LPS) molecule. The amphiphilic structure of LPS poses a challenge when using classical detection methods, which do not take into account its lipoglycan biochemistry. Typically, detection of LPS requires heat or chemical treatment of samples and relies on bioactivity assays for the conserved lipid A portion of the molecule. Our goal was to develop assays to facilitate the direct and discriminative detection of the entire LPS molecule and its O antigen in complex matrices using minimal sample processing. To perform serogroup identification of LPS, we used a method called membrane insertion on a waveguide biosensor, and tested three serogroups of LPS. The membrane insertion technique allows for the hydrophobic association of LPS with a lipid bilayer, where the exposed O antigen can be targeted for specific detection. Samples of beef lysate were spiked with LPS to perform O antigen specific detection of LPS from <i>E</i>. <i>coli</i> O157. To validate assay performance, we evaluated the biophysical interactions of LPS with lipid bilayers both in- and outside of a flow cell using fluorescence microscopy and fluorescently doped lipids. Our results indicate that membrane insertion allows for the qualitative and reliable identification of amphiphilic LPS in complex samples like beef homogenates. We also demonstrated that LPS-induced hole formation does not occur under the conditions of the membrane insertion assays. Together, these findings describe for the first time the serogroup-specific detection of amphiphilic LPS in complex samples using a membrane insertion assay, and highlight the importance of LPS molecular conformations in detection architectures.</p></div

A Modified Laboratory Approach to Determine Reaeration Rate for River Water

It is reported that reaeration rates determined from laboratory investigation may not suit well in predicting reaeration rate of natural streams. Sampling method during reaeration experiment is a potential source of error in laboratory estimation of reaeration rate coefficient for river waters, which has been addressed in this research. A modified method based on sampling procedure in a flume was adopted to develop a reaeration rate equation for Pusu River in Malaysia,which is demographically a very important river. An important feature including several culverts along the course of the river was also considered to model dissolved oxygen (DO) concentration.DOwas calibrated and validated using water quality analysis simulation program (WASP) considering appropriate kinetic rate coefficients for Pusu River. Performance of the new reaeration rate equation and other process equations in the calibration and validation data was assessed in terms of root-mean-square error (RMSE), mean error between observed and predicted data and R2 value. Study results revealed that the equation developed in B Abdullah Al-Mamun [email protected] Md Nuruzzaman [email protected] http://waterzaman.weebly.com/ Md Noor Bin Salleh [email protected] 1 Department of Civil Engineering, Rangpur Engineering College, Rangpur 5403, Bangladesh 2 Department of Civil Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), 53100 Kuala Lumpur, Malaysia 3 Bioenvironmental Engineering Research Center (BERC), Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), 53100 Kuala Lumpur, Malaysia this research considering the impact of culverts on reaeration rate predicted DO in Pusu River with improved accuracy as compared to the other equations. RMSEs were found to be 0.083 and 0.067 mg/L for calibration and validation data, respectively. Mean errors of observed and model-predicted data were 0.06 and 0.05 mg/L for calibration and validation,respectively. The R2 value was 0.99 in both cases. The study results facilitate accuracy in future studies on DO of Pusu River