65 research outputs found
Quantifying the interdisciplinarity of scientific journals and fields
There is an overall perception of increased interdisciplinarity in science,
but this is difficult to confirm quantitatively owing to the lack of adequate
methods to evaluate subjective phenomena. This is no different from the
difficulties in establishing quantitative relationships in human and social
sciences. In this paper we quantified the interdisciplinarity of scientific
journals and science fields by using an entropy measurement based on the
diversity of the subject categories of journals citing a specific journal. The
methodology consisted in building citation networks using the Journal Citation
Reports database, in which the nodes were journals and edges were established
based on citations among journals. The overall network for the 11-year period
(1999-2009) studied was small-world and scale free with regard to the
in-strength. Upon visualizing the network topology an overall structure of the
various science fields could be inferred, especially their interconnections. We
confirmed quantitatively that science fields are becoming increasingly
interdisciplinary, with the degree of interdisplinarity (i.e. entropy)
correlating strongly with the in-strength of journals and with the impact
factor.Comment: 23 pages, 6 figure
Flow cytometry immunophenotyping for diagnostic orientation and classification of pediatric cancer based on the EuroFlow Solid Tumor Orientation Tube (STOT)
Early diagnosis of pediatric cancer is key for adequate patient management and improved outcome. Although multiparameter flow cytometry (MFC) has proven of great utility in the diagnosis and classification of hematologic malignancies, its application to non-hematopoietic pediatric tumors remains limited. Here we designed and prospectively validated a new single eight-color antibody combination-solid tumor orientation tube, STOT-for diagnostic screening of pediatric cancer by MFC. A total of 476 samples (139 tumor mass, 138 bone marrow, 86 lymph node, 58 peripheral blood, and 55 other body fluid samples) from 296 patients with diagnostic suspicion of pediatric cancer were analyzed by MFC vs. conventional diagnostic procedures. STOT was designed after several design-test-evaluate-redesign cycles based on a large panel of monoclonal antibody combinations tested on 301 samples. In its final version, STOT consists of a single 8-color/12-marker antibody combination (CD99-CD8/(nu)myogenin/CD4-EpCAM/CD56/GD2/(sm)CD3-CD19/(cy)CD3-CD271/CD45). Prospective validation of STOT in 149 samples showed concordant results with the patient WHO/ICCC-3 diagnosis in 138/149 cases (92.6%). These included: 63/63 (100%) reactive/disease-free samples, 43/44 (98%) malignant and 4/4 (100%) benign non-hematopoietic tumors together with 28/38 (74%) leukemia/lymphoma cases; the only exception was Hodgkin lymphoma that required additional markers to be stained.& nbsp;In addition, STOT allowed accurate discrimination among the four most common subtypes of malignant CD45(-) CD56(++) non-hematopoietic solid tumors: 13/13 (GD2(++) (nu)myogenin(-) CD271(-/+) (nu)MyoD1(-) CD99(-) EpCAM(-)) neuroblastoma samples, 5/5 (GD2(-) (nu)myogenin(++) CD271(++) (nu)MyoD1(++) CD99(-/+) EpCAM(-)) rhabdomyosarcomas, 2/2 (GD2(-/+) (nu)myogenin(-) CD271(+) (nu)MyoD1(-) CD99(+) EpCAM(-)) Ewing sarcoma family of tumors, and 7/7 (GD2(-) (nu)myogenin(-) CD271(+) (nu)MyoD1(-) CD99(-) EpCAM(+)) Wilms tumors. In summary, here we designed and validated a new standardized antibody combination and MFC assay for diagnostic screening of pediatric solid tumors that might contribute to fast and accurate diagnostic orientation and classification of pediatric cancer in routine clinical practice.Stemcel biology/Regenerative medicine (incl. bloodtransfusion
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