Maize production under combined conservation agriculture and integrated soil fertility management in the sub-humid and semi-arid regions of Kenya

Open Access Article; Published online: 23 May 2020Crop production in Sub-Saharan Africa (SSA) is constrained by rainfall variability and declining soil fertility. This has over time led to a decrease in crop yield, among them also maize. This decrease is also experienced in the sub-humid and semi-arid locations of Kenya. Among the commonly used soil and water management practices in SSA are Conservation Agriculture (CA) and integrated soil fertility management (ISFM). Crop response to these management practices is influenced by the existence of soil fertility gradients which are common among smallholder farmers. This paper presents results from a study done in the sub-humid and semi-arid location of Kenya, focusing on the effects of CA- and/or ISFM-based practices on maize yield. Trials were set out on farms within the two locations using a one farm one replicate randomized design. In each farm, CA-based treatment, no tillage with residue retention (NTR), ISFM-based treatment, conventional tillage with use of manure (CTM), a combination of CA + ISFM, no tillage with residue retention and use of manure (NTRM) and a control, (C) were laid down on fields representing high and low fertility soils. The trials started in the long rains of 2017 (LR2017) running for four seasons i.e., LR2017, short rains 2017 (SR2017), long rains 2018 (LR2018) and short rains 2018 (SR2018). Soil water content (SWC) and nitrogen use efficiency (NUE) were also monitored and evaluated. In either high or low fertility fields, maize grain yield was significantly different between the control and both NTR, CTM and NTRM with no significant differences between NTR, CTM and NTRM. Maize grain yield increase compared to the control was highest under ISFM in the low fertility fields in both locations and all seasons. For example, during the last season, SR2018, NTR, CTM and NTRM significantly increased maize grain yield by 136 %, 297 %, and 208 %, respectively, compared to the control, in the low fertility fields of sub-humid Kibugu. In the semi-arid Machang’a, the increase by NTR, CTM and NTRM, respectively, in the low fertility fields was 146 %, 379 % and 183 % for SR2018. This was linked to the tendency of ISFM to improve crop yield in the short run. For both locations, SWC and NUE were highest under NTR. In the sub-humid Kibugu, during SR2018, at the grain filling stage, 78 days after sowing, SWC under NTR, CTM and NTRM was higher by 16 %, 9 % and 20 %, respectively, compared to the control. Also at 78 days after sowing, in the semi-arid Machang’a, SWC was 18 %, 7 % and 15 % significantly higher under NTR, CTM and NTRM, respectively, compared to the control. The higher SWC observed under NTR and NTRM was related to no tillage with residue retention while under CTM it was related to improved soil organic matter through manure addition. NUE, on the other hand, was 26 % and 23 % in Kibugu and Machang’a, respectively, and lowest under the combined practice (NTRM), i.e., 19 % and 15 % in Kibugu and Machang’a, respectively. The high NUE under CA was attributed to the placement of urea in the planting holes while maintaining residue on the soil surface. The low NUE under NTRM was linked to fertilizer N immobilization. Lastly, from the biomass yield, our study showed that monocrop maize under NTR requires a kick-starting by an ISFM-based practice in the low fertility fields of the semi-arid region

Striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal α-glucosidase and Schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene

AbstractSequence comparison of the primary structure of the yeast Schwanniomyces occidentalis glucoamylase (GAM) with GAMs in different microorganisms did not reveal significant similarities. By contrast, striking similarities were, surprisingly, found with 3 mammalian secretory and integral membrane proteins: the 2 subunits of intestinal brush border sucrase-isomaltase and human lysosomal α-glucosidase. The similarities among these proteins are found as clusters of up to 8 amino acids and distributed all over the protein sequences. The major sequence differences are found in the N-terminal regions accounting, probably, for the different cellular locations of these proteins. The high level of similarities between sucrase, isomaltase, Sch. occidentalis GAM and human lysosomal α-glucosidase suggest that these proteins are derived from the same ancestral gene. To our knowledge, this is the first report that describes similarities between a yeast secretory protein and mammalian secretory and integral membrane proteins

Surrogate endpoints in liver surgery related trials: a systematic review of the literature

AbstractBackgroundAlthough the safety of liver surgery has improved enormously, hepatic surgery continues to face challenging complications. Therefore, improvements supported by evidence‐based guidelines are still required. The conduct of randomized controlled trials in liver surgery using dichotomous outcomes requires a large sample size. The use of surrogate endpoints (SEPs) reduces sample size but SEPs should be validated before use.AimThe aim of this review was to summarize the SEPs used in hepatic surgery related trials, their definitions and recapitulating the evidence validating their use.MethodA systematic computerized literature search in the biomedical database PubMed using the MeSH terms ‘hepatectomy’ or ‘liver resection’ or ‘liver transection’ was conducted. Search was limited to papers written in the English language and published between 1 January 2000 and 1 January 2010.ResultsA total of 593 articles met the search terms and 49 articles were included in the final selection. Standard biochemical liver functions tests were the most frequently used SEP (32 of 49 the studies). The used definitions of SEPs varied greatly among the studies. Most studies referred to earlier published material to justify their choice of SEP. However, no validating studies were found.ConclusionMany SEPs are used in liver surgery trials however there is little evidence validating them

Physico-chemical soil attributes under conservation agriculture and integrated soil fertility management

Open Access Article; Published online: 25 Apr 2021Conservation Agriculture (CA) and Integrated Soil Fertility Management (ISFM) have been promoted in Sub Saharan Africa as a means to improve soil quality. A four season research (March, 2017 to March, 2019) was conducted to evaluate CA-based treatment, no tillage with residue retention (NTR), ISFM-based treatment, conventional tillage with use of manure (CTM), a combination of CA + ISFM, no tillage with residue retention and use of manure (NTRM) and a control, (C) on soil quality attributes. In the two locations (sub-humid and semi-arid) the effect of soil fertility gradients (high and low) were considered. Trials were set out using a one farm one replicate randomized design. In either high or low fertility fields, soil chemical and physical properties were significantly different between the control and NTR, CTM and NTRM with no significant differences between NTR, CTM and NTRM. SOC was higher under NTR and NTRM practices, which consequently had higher hydraulic conductivity, air permeability, mean weight diameter and available phosphorus. For all the treatments and in both locations, the low fertility fields had significantly lower agronomic use efficiency (AUE) compared to the high fertility fields. In both soil types, plant available water capacity and relative water capacity values were below the recommended thresholds indicating low soil water uptake, suboptimal microbial activity and consequently low nutrient uptake which explains the observed low AUE

Circulating Tumour DNA as Biomarker for Colorectal Liver Metastases:A Systematic Review and Meta-Analysis

Circulating tumour DNA (ctDNA) is a potential biomarker that could contribute to more judicious patient selection for personalised treatment. This review and meta-analysis gives an overview of the current knowledge in the literature investigating the value of ctDNA in patients with colorectal liver metastases (CRLM). A systematic search was conducted in electronic databases for studies published prior to the 26th of May 2023. Studies investigating the association between ctDNA and oncological outcomes in patients undergoing curative-intent local therapy for CRLM were included. Meta-analyses were performed to pool hazard ratios (HR) for the recurrence-free survival (RFS) and overall survival (OS). A total of eleven studies were included and nine were eligible for meta-analyses. Patients with detectable ctDNA after surgery experienced a significantly higher chance of recurrence (HR 3.12, 95% CI 2.27–4.28, p &lt; 0.000010) and shorter OS (HR 5.04, 95% CI 2.53–10.04, p &lt; 0.00001) compared to patients without detectable ctDNA. A similar association for recurrence was found in patients with detectable ctDNA after the completion of adjuvant therapy (HR 6.39, 95% CI 2.13–19.17, p &lt; 0.0009). The meta-analyses revealed no association between detectable ctDNA before surgery and the RFS and OS. These meta-analyses demonstrate the strong association between detectable ctDNA after treatment and oncological outcomes in CRLM patients.</p

The effect of vegetation patterns on Aeolian mass flux at regional scale: A wind tunnel study

ABSTRACT Although insight on the effect of vegetation pattern on Aeolian mass transport is essential for re-planting degraded land, only limited knowledge on this effect is available. The objective of this research was to understand the effect of vegetation design on the Aeolian mass flux inside a single land unit and at the borders among land units. A simulation of Atriplex halimus shrubs inside a wind tunnel was made, and sand redistribution was measured after the application of 200-230 seconds wind at a speed of 11 ms-1. The study showed that: 1) sediment maximum transport inside a single land unit is related to the neighboring land units and to the vegetation pattern within both the unit itself and the neighboring land units; 2) the effect of neighboring land units includes the protection effect and the ruling of sediment crossing from one land unit to the neighboring land units; 3) for the designing of re-planting of degraded land the ‘streets’ (zones of erosion areas similar to streets) effect need to be considered; and 4) in addition to the general knowledge needed on the effect of vegetation pattern on the erosion and deposition within an area, it is important to have insight on the redistribution of sediment at small scales upon the aim of the project. Key words: wind erosion; vegetation pattern; wind-blown mass transport; wind tunne

Effects of oral meal feeding on whole body protein breakdown and protein synthesis in cachectic pancreatic cancer patients

Background: Pancreatic cancer is often accompanied by cachexia, a syndrome of severe weight loss and muscle wasting. A suboptimal response to nutritional support may further aggravate cachexia, yet the influence of nutrition on protein kinetics in cachectic patients is poorly understood. Methods: Eight cachectic pancreatic cancer patients and seven control patients received a primed continuous intravenous infusion of l‐[ring‐2H5]phenylalanine and l‐[3,3‐2H2]tyrosine for 8 h and ingested sips of water with l‐[1‐13C]phenylalanine every 30 min. After 4 h, oral feeding was started. Whole body protein breakdown, protein synthesis, and net protein balance were calculated. Results are given as median with interquartile range. Results: Baseline protein breakdown and protein synthesis were higher in cachectic patients compared with the controls (breakdown: 67.1 (48.1–79.6) vs. 45.8 (42.6–46.3) µmol/kg lean body mass/h, P = 0.049; and synthesis: 63.0 (44.3–75.6) vs. 41.8 (37.6–42.5) µmol/kg lean body mass/h, P = 0.021). During feeding, protein breakdown decreased significantly to 45.5 (26.9–51.1) µmol/kg lean body mass/h (P = 0.012) in the cachexia group and to 33.7 (17.4–37.1) µmol/kg lean body mass/h (P = 0.018) in the control group. Protein synthesis was not affected by feeding in cachectic patients: 58.4 (46.5–76.1) µmol/kg lean body mass/h, but was stimulated in controls: 47.9 (41.8–56.7) µmol/kg lean body mass/h (P = 0.018). Both groups showed a comparable positive net protein balance during feeding: cachexia: 19.7 (13.1–23.7) and control: 16.3 (13.6–25.4) µmol/kg lean body mass/h (P = 0.908). Conclusion: Cachectic pancreatic cancer patients have a higher basal protein turnover. Both cachectic patients and controls show a comparable protein anabolism during feeding, albeit through a different pattern of protein kinetics. In cachectic patients, this is primarily related to reduced protein breakdown, whereas in controls, both protein breakdown and protein synthesis alterations are involved