In vitro behaviour of curcumin nanoemulsions stabilized by biopolymer emulsifiers: Effect of interfacial composition

Nanoemulsions present a high potential to be used in food products due to their advantages over conventional emulsions (e.g. higher stability to gravitational separation and droplet aggregation and enhanced bioavailability of encapsulated compounds), however their application to foods is hindered by some concerns about potential risks associated with their ingestion. The knowledge of the behaviour of nanoemulsions as well as the fate of bioactive compounds encapsulated within them in the gastrointestinal (GI) tract is of utmost importance to assess their safety for human consumption and to produce delivery systems that provide an optimized bioactivity of the encapsulated compound. In this work, a dynamic gastrointestinal model, comprising the simulation of stomach, duodenum, jejunum and ileum, was used to evaluate the behaviour of curcumin nanoemulsions stabilized by biopolymer emulsifiers (lactoferrin and lactoferrin/alginate multilayer structure) under GI conditions. The interfacial characteristics of curcumin nanoemulsions had a significant impact on their physicochemical stability within the simulated GI tract. Also, results suggested that alginate coating may be able to control the rate of lipid digestion and free fatty acids adsorption within the GI tract, but the encapsulated lipid is digested at the same extent, releasing the lipophilic bioactive compound. This work contributes to an improved understanding of how multilayer nanoemulsions behave within the GI tract and this knowledge will be useful for the optimization of delivery systems that improve the physicochemical stability of emulsions in food products, while still releasing encapsulated lipophilic bioactive compounds.FCT Strategic Project of UID/BIO/04469/2013 unit, the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462) and the project “ BioInd e Biotechnology and Bioengineering for improved Industrial and Agro-Food processes ” , REF. NORTE-07-0124-FEDER-000028 Co-funded by the Programa Operacional Regional do Norte (ON.2 e O Novo Norte), QREN, FEDER. Thanks are also due to FCT for the fi nancial support of the Research Unit 395 62/94-QOPNA (project PEst-C/QUI/UI0062/2013; FCOMP-01-0124-FEDER-037296)

The elaboration of indices to assess biological water quality. A case study

Here we tested the application of a nodal analysis for the elaboration of biotic indices for particular stressing conditions. The work was carried out in an intermittent Mediterranean stream where superficial flow was absent during summer. The river was perturbed by an effluent with high pH, sulphates, nitrates and conductivity. "Summer" and "winter" samples were treated separately. We first identified groups of sites differing in taxonomical composition by cluster analysis. Then we tested whether groups of sites also differed in their abiotic characteristics. In the following step, groups of cooccurring taxa were also identified by cluster analysis. The indicator value of a taxa group was measured by fidelity measurements for site groups. Indicator taxa were incorporated in a water quality table. The biotic index in the water quality table clearly discriminated impacted from reference sites in the two following years and was correlated with the first axis of a correspondence analysis biplot which also discriminated impacted from clean sites. We suggest that nodal analysis can be a reliable technique for the identification of bioindicators and the elaboration of biotic indices.http://www.sciencedirect.com/science/article/B6V73-3SX5H1V-F/1/2ddcd6137e173976c270b458bbb4c99

The elaboration of indices to assess biological water quality. A case study

Here we tested the application of a nodal analysis for the elaboration of biotic indices for particular stressing conditions. The work was carried out in an intermittent Mediterranean stream where superficial flow was absent during summer. The river was perturbed by an effluent with high pH, sulphates, nitrates and conductivity. "Summer" and "winter" samples were treated separately. We first identified groups of sites differing in taxonomical composition by cluster analysis. Then we tested whether groups of sites also differed in their abiotic characteristics. In the following step, groups of cooccurring taxa were also identified by cluster analysis. The indicator value of a taxa group was measured by fidelity measurements for site groups. Indicator taxa were incorporated in a water quality table. The biotic index in the water quality table clearly discriminated impacted from reference sites in the two following years and was correlated with the first axis of a correspondence analysis biplot which also discriminated impacted from clean sites. We suggest that nodal analysis can be a reliable technique for the identification of bioindicators and the elaboration of biotic indices.http://www.sciencedirect.com/science/article/B6V73-3SX5H1V-F/1/2ddcd6137e173976c270b458bbb4c99

Characterization of Plum Procyanidins by Thiolytic Depolymerization

The phenolic compounds of ?Green Gage? (GG) plums (Prunus domestica L.), ?Rainha Cla?udia Verde?, from a ?protected designation of origin? (PDO), in Portugal, were quantified in both flesh and skin tissues of plums collected in two different orchards (GG-V and GG-C). Analyzes of phenolic compounds were also performed on another GG European plum obtained in France (GG-F) and two other French plums, ?Mirabelle? (M) and ?Golden Japan? (GJ). Thiolysis was used for the first time in the analysis of plum phenolic compounds. This methodology showed that the flesh and skin contain a large proportion of flavan-3-ols, which account, respectively, for 92 and 85% in GJ, 61 and 44% in GG-V, 62 and 48% in GG-C, 54 and 27% in M, and 45 and 37% in GG-F. Terminal units of procyanidins observed in plums are mainly (+)-catechin (54?77% of all terminal units in flesh and 57?81% in skin). The GJ plums showed a phenolic composition different from all of the others, with a lower content of chlorogenic acid isomers and the presence of A-type procyanidins as dimers and terminal residues of polymerized forms. The average degree of polymerization (DPn) of plum procyanidins was higher in the flesh (5?9 units) than in the skin (4?6 units). Procyanidin B7 was observed in the flesh of all GG plums and in the skin of the Portuguese ones. Principal component analysis of the phenolic composition of the flesh and skin of these plums obtained after thiolysis allowed their distinction according to the variety and origin, opening the possibility of the use of phenolic composition for variety/origin identification