Multiple roles of phosphoinositide-specific phospholipase C isozymes.

Phosphoinositide-specific phospholipase C is an effector molecule in the signal transduction process. It generates two second messengers, inositol-1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. Currently, thirteen mammal PLC isozymes have been identified, and they are divided into six groups: PLC-beta, -gamma, -delta, -epsilon, -zeta and -eta. Sequence analysis studies demonstrated that each isozyme has more than one alternative splicing variant. PLC isozymes contain the X and Y domains that are responsible for catalytic activity. Several other domains including the PH domain, the C2 domain and EF hand motifs are involved in various biological functions of PLC isozymes as signaling proteins. The distribution of PLC isozymes is tissue and organ specific. Recent studies on isolated cells and knockout mice depleted of PLC isozymes have revealed their distinct phenotypes. Given the specificity in distribution and cellular localization, it is clear that each PLC isozyme bears a unique function in the modulation of physiological responses. In this review, we discuss the structural organization, enzymatic properties and molecular diversity of PLC splicing variants and study functional and physiological roles of each isozyme

Photosynthetic pigment levels in Macroptilium lathyroides L under water deficit.

Título em espanhol: Contenido de pigmentos fotosinteticos Macroptilium lathyroides L en condiciones de sequia

INTEGRAL observations of the field of the BL Lacertae object S5~0716+714

We have performed observations of the blazar S5 0716+714 with INTEGRAL on 2-6 April 2004. In the first months of 2004, the source had increased steadily in optical brightness and had undergone two outbursts. During the latter, occurred in March, it reached the extreme level of R = 12.1 mag, which triggered our INTEGRAL program. The target has been detected with IBIS/ISGRI up to 60 keV, with a flux of ~3 x 10e-11 erg/s/cm2 in the 30-60 keV interval, a factor of ~2 higher than observed by the BeppoSAX PDS in October 2000. In the field of S5 0716+714 we have also detected the Flat Spectrum Radio Quasar S5 0836+710 and the two Seyfert galaxies Mkn 3 and Mkn 6. Their IBIS/ISGRI spectra are rather flat, albeit consistent with those measured by BeppoSAX. In the spectrum of Mkn 3 we find some evidence of a break between ~60 and ~100 keV, reminiscent of the high energy cut-offs observed in other Seyfert galaxies. This is the first report of INTEGRAL spectra of weak Active Galactic Nuclei.Comment: 5 pages, 5 figures, in press in A&

Energy funneling in a bent chain of Morse oscillators with long-range coupling

A bent chain of coupled Morse oscillators with long-range dispersive interaction is considered. Moving localized excitations may be trapped in the bending region. Thus chain geometry acts like an impurity. An energy funneling effect is observed in the case of random initial conditions.Comment: 6 pages, 12 figures. Submitted to Physical Review E, Oct. 13, 200

Novas Estirpes de Bradyrizobium para o Incremento da Produtividade de Genótipos de Soja em Terras Baixas.

bitstream/item/207893/1/Boletim-330.pd

First INTEGRAL Observations of Eight Persistent Neutron Star Low Mass X-ray Binaries

Early results from the INTEGRAL Core Program, for a sample of eight persistently bright neutron star low mass X-ray binaries in the energy range from 5 keV to 200 keV are presented. It is shown that INTEGRAL efficiently detects sources and that spectra may be obtained up to several hundreds of keV by combining data from three of the four INTEGRAL instruments: JEM-X, IBIS and SPI. For the source GX 17+2 it is shown that the spectrum extends well above 100 keV with a flattening of the spectrum above 30 keV. This might suggest a non-thermal comptonisation emission, but uncertainties in the current data reduction and background determination do not allow firm conclusions to be drawn.Comment: 5 pages, 6 figures, accepted for publication in Astronomy & Astrophysic

Resolving the Hard X-ray Emission of GX 5-1 with INTEGRAL

We present the study of one year of INTEGRAL data on the neutron star low mass X-ray binary GX 5-1. Thanks to the excellent angular resolution and sensitivity of INTEGRAL, we are able to obtain a high quality spectrum of GX 5-1 from ~5 keV to ~100 keV, for the first time without contamination from the nearby black hole candidate GRS 1758-258 above 20 keV. During our observations, GX 5-1 is mostly found in the horizontal and normal branch of its hardness intensity diagram. A clear hard X-ray emission is observed above ~30 keV which exceeds the exponential cut-off spectrum expected from lower energies. This spectral flattening may have the same origin of the hard components observed in other Z sources as it shares the property of being characteristic to the horizontal branch. The hard excess is explained by introducing Compton up-scattering of soft photons from the neutron star surface due to a thin hot plasma expected in the boundary layer. The spectral changes of GX 5-1 downward along the "Z" pattern in the hardness intensity diagram can be well described in terms of monotonical decrease of the neutron star surface temperature. This may be a consequence of the gradual expansion of the boundary layer as the mass accretion rate increases.Comment: 10 pages, 17 figures, accepted for publication in A&

Single Molecule Statistics and the Polynucleotide Unzipping Transition

We present an extensive theoretical investigation of the mechanical unzipping of double-stranded DNA under the influence of an applied force. In the limit of long polymers, there is a thermodynamic unzipping transition at a critical force value of order 10 pN, with different critical behavior for homopolymers and for random heteropolymers. We extend results on the disorder-averaged behavior of DNA's with random sequences to the more experimentally accessible problem of unzipping a single DNA molecule. As the applied force approaches the critical value, the double-stranded DNA unravels in a series of discrete, sequence-dependent steps that allow it to reach successively deeper energy minima. Plots of extension versus force thus take the striking form of a series of plateaus separated by sharp jumps. Similar qualitative features should reappear in micromanipulation experiments on proteins and on folded RNA molecules. Despite their unusual form, the extension versus force curves for single molecules still reveal remnants of the disorder-averaged critical behavior. Above the transition, the dynamics of the unzipping fork is related to that of a particle diffusing in a random force field; anomalous, disorder-dominated behavior is expected until the applied force exceeds the critical value for unzipping by roughly 5 pN.Comment: 40 pages, 18 figure