1,962 research outputs found
Análise e comparação filogenética de expansinas presentes em Urochloa decumbens cv Basilick.
O objetivo deste trabalho foi analisar 4 expansinas de Urochloa decumbens, verificando a similaridade e a homologia delas com as de outras plantas
Optical sensing in microchip capillary electrophoresis by femtosecond laser written waveguides
Capillary electrophoresis separation in an on-chip integrated microfluidic channel is typically monitored with bulky, bench-top optical excitation/detection instrumentation. Optical waveguides allow confinement and transport of light in the chip directing it to a small volume of the microfluidic channel and collecting the emitted/transmitted radiation. However, the fabrication of optical waveguides or more complex photonic components integrated with the microfluidic channels is not a straightforward process, since it requires a localized increase of the refractive index of the substrate.\ud
Recently, a novel technique has emerged for the direct writing of waveguides and photonic circuits in transparent glass substrates by focused femtosecond laser pulses.\ud
In this work we demonstrate the integration of femtosecond laser written optical waveguides into a commercial microfluidic chip. We fabricate high quality waveguides intersecting the microchannels at arbitrary positions and use them to optically address with high spatial selectivity their content. In particular, we apply our technique to integrate optical detection in microchip capillary electrophoresis. Waveguides are inscribed at the end of the separation channel in order to optically excite the different plugs reaching that point; fluorescence from the labelled biomolecules crossing the waveguide output is efficiently collected at a 90° angle by a high numerical aperture optical fiber. The sensitivity of the integrated optical detection system was first evaluated filling the chip with a dye solution, obtaining a minimum detectable concentration of 40 pM. \ud
After dynamic coating of the microchannels with an EPDMA polymer we demonstrate electrophoresis of an oligonucleotide plug with concentration down to 1 nM and wavelength-selective monitoring of on-chip separation of three fluorescent dyes. Work is in progress on separation and detection of fluorescent-labeled DNA fragments, targeting specific, diagnostically relevant regions of a template DNA, for application to the detection of chromosome aberrations
Família defensiva de peptídeos antimicrobianos em Urochloa.
O capim-braquiária é muito utilizado no Brasil, principalmente no cerrado, como forrageira devido a sua alta adaptabilidade a variados solos, como solos ácidos ou pobres em nutrientes; e apresenta alta cobertura e produção de biomassa. Os peptídeos antimicrobianos são moléculas proteicas pequenas produzidas por todos os seres vivos como parte do sistema imune inato para combater infecções e patógenos. Peptídeos antimicrobianos vegetais possuem um amplo espectro de atividades antimicrobianas contra fitopatógenos e a grande maioria deles é ativa contra fungos, enquanto alguns são inibidores de bactérias e insetos herbívoros. Deste modo, o objetivo deste trabalho foi verificar e identificar a presença de peptídeos antimicrobianos da família das defensinas em U. decumbens por meio da comparação entre as sequências do transcriptoma da planta contra as sequências de aminoácidos de peptídeos antimicrobianos. O transcriptoma da planta foi fornecido pela EMBRAPA Gado de Corte (Campo Grande, MS), obtido pela extração de RNA de plantas expostas a condições de estresse (com alumínio) e sem estresse (sem alumínio). As sequências dos peptídeos antimicrobianos foram baixadas do banco de dados online CAMP e foram formatadas em FASTA em um banco local no computador da FCBA, UFGD. Foi utilizado o blast local para fazer o alinhamento entre os 164.920 transcritos únicos da U. decumbens e as 5.906 sequências de peptídeos antimicrobianos. Deste alinhamento obteve-se 3.161 similaridades com 251 peptídeos diferentes. Para a análise dos resultados utilizou-se o MySQL, separando-os em famílias de acordo com a classificação do banco de dados CAMP e selecionando um grupo amostral para a construção das árvores filogenéticas. A partir desta análise, obteve-se 261 similaridades com 52 peptídeos diferentes dentro da família das defensinas. A maioria dos peptídeos antimicrobianos identificados possui atividade antifúngica ou antimicrobiana, e são de origem vegetal o que condiz com os transcritos da gramínea
Variabilidade genética e taxa de cruzamento em progênies da população de melhoramento de Stylosanthes guianensis.
bitstream/item/110778/1/BP32-em-alta.pd
Comparação de três métodos de extração de DNA genômico para análises moleculares em Stylosanthes guianensis.
O objetivo deste trabalho foi comparar a quantidade e qualidade do DNA isolado de folhas de S. guianensis pelos métodos de Bonato et al. (2002), de Faleiro et al. (2003) e um método baseado no de Bonato et al. (2002), mas com modificações que resultem um DNA mais puro e em quantidade suficiente para execução de diversas análises moleculares, incluindo amplificação, clonagem e sequenciamento.bitstream/CNPGC-2010/13216/1/CT36.pd
Afatinib in the first-line treatment of patients with non-small cell lung cancer: clinical evidence and experience
Epidermal growth factor receptor (EGFR) gene mutations identify a molecularly defined subset of non-small cell lung cancer (NSCLC) patients who display an excellent sensitivity to EGFR tyrosine kinase inhibitors (TKIs). First-generation reversible EGFR TKIs, gefitinib and erlotinib have been proven to improve the objective response rate and to prolong the progression-free survival compared with standard chemotherapy in large phase III trials. Unfortunately, virtually all patients develop resistance to treatment, usually within 9–12 months. Afatinib is an irreversible ErbB family inhibitor initially designed to overcome the development of resistance. Compared with gefitinib in a first-line setting, afatinib prolonged progression-free survival and time to treatment failure, without impacting on overall survival in the general population of EGFR-mutant patients. However, afatinib has been shown to prolong overall survival in the subset of patients with an EGFR exon 19 deletion compared with chemotherapy. The aim of this review is to summarize the clinical evidence available to date and to critically discuss the place in therapy of afatinib in the rapidly expanding landscape of EGFR-mutant NSCLC first-line therapy
Avaliação agronômica e seleção de acessos da leguminosa forrageira Stylosanthes guianensis em Mato Grosso do Sul
Quantum-critical spin dynamics in quasi-one-dimensional antiferromagnets
By means of nuclear spin-lattice relaxation rate 1/T1, we follow the spin
dynamics as a function of the applied magnetic field in two gapped
one-dimensional quantum antiferromagnets: the anisotropic spin-chain system
NiCl2-4SC(NH2)2 and the spin-ladder system (C5H12N)2CuBr4. In both systems,
spin excitations are confirmed to evolve from magnons in the gapped state to
spinons in the gapples Tomonaga-Luttinger-liquid state. In between, 1/T1
exhibits a pronounced, continuous variation, which is shown to scale in
accordance with quantum criticality. We extract the critical exponent for 1/T1,
compare it to the theory, and show that this behavior is identical in both
studied systems, thus demonstrating the universality of quantum critical
behavior
Bronchiolitis obliterans organizing pneumonia after radiation therapy for lung cancer. A case report
Bronchiolitis obliterans organizing pneumonia (BOOP), also known as cryptogenic organizing pneumonia, has mainly been described in patients with breast cancer who received radiotherapy after breast-conserving surgery. In this rare case, a 70-year-old man with left apical squamous lung cancer developed BOOP after radiotherapy and only one cycle of concomitant chemotherapy. This case report draws attention to the development of this syndrome in the unusual setting of lung cancer, advising prompt steroid treatment when diagnostic images reveal the characteristic signs of the disease
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