ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF LEUCAS ZEYLANICA LINN BY USING ISOLATED FROG HEART

Objective: The present study was aimed to evaluate the antioxidant activity of methanolic extract of leaves of Leucas zeylanica Linn. by using isolated frog heart as a model.Method: 1mM  of H2O2 solution in frog Ringer solution was used to induce oxidative stress on isolated frog heart. When ringer solution containing H2O2 perfused to frog heart  preparation, which indicating the induction of oxidative stress on frog heart, this might be due to desitilization of receptors. Cardiac output, heart rate and cardiac arrest parameters were estimated.Results: The present study results supports that the frog heart model for induction of oxidative stress by H2O2. It shows negative ionotropic and chronotopic effects and the cardiac arrest was produced at 20th minute. In the presence of methanolic extract of leaves of Leucas zeylanica,  the cardiac arrest was observed at  38th minutes i.e. heart was protected longer period that indicates antioxidant activity which was compared with the standard ascorbic acid.                                  Conclusion: The results obtained in this work showed that methanolic extract of leaves of  Leucas zeylanica exhibits anti-oxidant activity against H2O2 induced oxidative stress on isolated frog heart model and compared with a standard antioxidant agent (Ascorbic acid). Key words: Frog heart, antioxidant activity, Leucas zeylanica, methanolic extract, oxidative stress

Simulation of High Step-Up Resonant Parallel LC Converter for Grid Connected Renewable Energy Sources

With the rapid improvement of large-scale renewable energy sources and HVDC grid, it is a capable alternative to connect the renewable energy sources to the HVDC grid with a pure dc system, in which high-power high-voltage step-up dc–dc converters are the key equipment to transmit the electrical energy. This paper presents a High step up LC converter it can achieve high voltage gain using an LC parallel resonant tank.Also provided zero voltage switching (ZVS) technology under switch turn-On condition also at turn-Off conditions at main power switches by rectifying diodes to reduce the conduction losses. The operation principle of the converter and its resonant parameter selection is presented in this paper. The operation principle of the converter has been successfully verified with the help of MATLAB/SIMULINK

Image Blur Detection Using Local Power Spectrum

In this paper work, blur detection of images is carried with local power spectrum. De blurring of image plays a important role in image processing and computer vision techniques. In deblurring of image, the first step is considered the input image as a motion blurred image. Our blur detection is based on block by block local mean calculation. After that find out the global mean for the blurred image, then comparison of local mean with global mean takes place. The experimental result shows that the robustness of proposed algorithm. The proposed method performing operations on image for detecting blurred regions. After that detected blurred content converted in to an un blurred region that shows the final output of this method

A CRYPTOGRAPHIC PRIMITIVE TO PREVENT AN INVADER FROM CREATING DODGING ATTACKS

Keyed invasion recognition method is a charge card applicatoin-layer network system of anomaly recognition that extracts several features from all the payload. The essential idea of Keyed invasion recognition system to obstruct evasion attacks is always to are the thought of key, this like a secret element which determines extraction of classification features within the payload. Our focus remains on recovering key completely through efficient procedures, demonstrating that classification procedure leaks data regarding this which may be leveraged by means of an opponent. Inside our work we evaluate strength of Keyed Invasion Recognition System against key-recovery attacks. We describe that recovering of the end result is particularly simple when as long as the attacker can talk to Keyed invasion recognition system and get feedback regarding probing demands

Biofilter aquaponic system for nutrients removal from fresh market wastewater

Aquaponics is a significant wastewater treatment system which refers to the combination of conventional aquaculture (raising aquatic organism) with hydroponics (cultivating plants in water) in a symbiotic environment. This system has a high ability in removing nutrients compared to conventional methods because it is a natural and environmentally friendly system (aquaponics). The current chapter aimed to review the possible application of aquaponics system to treat fresh market wastewater with the intention to highlight the mechanism of phytoremediation occurs in aquaponic system. The literature revealed that aquaponic system was able to remove nutrients in terms of nitrogen and phosphorus

Genomic Analysis of QTLs and Genes Altering Natural Variation in Stochastic Noise

Quantitative genetic analysis has long been used to study how natural variation of genotype can influence an organism's phenotype. While most studies have focused on genetic determinants of phenotypic average, it is rapidly becoming understood that stochastic noise is genetically determined. However, it is not known how many traits display genetic control of stochastic noise nor how broadly these stochastic loci are distributed within the genome. Understanding these questions is critical to our understanding of quantitative traits and how they relate to the underlying causal loci, especially since stochastic noise may be directly influenced by underlying changes in the wiring of regulatory networks. We identified QTLs controlling natural variation in stochastic noise of glucosinolates, plant defense metabolites, as well as QTLs for stochastic noise of related transcripts. These loci included stochastic noise QTLs unique for either transcript or metabolite variation. Validation of these loci showed that genetic polymorphism within the regulatory network alters stochastic noise independent of effects on corresponding average levels. We examined this phenomenon more globally, using transcriptomic datasets, and found that the Arabidopsis transcriptome exhibits significant, heritable differences in stochastic noise. Further analysis allowed us to identify QTLs that control genomic stochastic noise. Some genomic QTL were in common with those altering average transcript abundance, while others were unique to stochastic noise. Using a single isogenic population, we confirmed that natural variation at ELF3 alters stochastic noise in the circadian clock and metabolism. Since polymorphisms controlling stochastic noise in genomic phenotypes exist within wild germplasm for naturally selected phenotypes, this suggests that analysis of Arabidopsis evolution should account for genetic control of stochastic variance and average phenotypes. It remains to be determined if natural genetic variation controlling stochasticity is equally distributed across the genomes of other multi-cellular eukaryotes

Detection of Escherichia coli O157:H7 prevalence in foods of animal origin by cultural methods and PCR technique

A total of 250 samples (50 each of beef, mutton and chicken and 50 samples each of beef swabs and mutton swabs) collected from various sources were subjected to PCR and cultural methods for the presence of Escherichia coli O157:H7. Primers for hlyA, stx1 & 2 genes were used for the detection of Escherichia coli O157:H7 and shiga toxins respectively. Out of 250 samples, 27 showed presence of Escherichia coli O157:H7 (5 beef, 6 beef swabs, 2 mutton, 12 mutton swabs and 2 chicken out of 50 samples each) by PCR where as only 11 samples (one beef, 2 beef swabs, 1 mutton, 6 mutton swabs and one chicken sample) were positive by cultural method. Of the 27 Escherichia coli O157:H7 positive samples by PCR, 12 showed stx1, 7 showed stx2 and 5 showed both stx1 and stx2. The sensitivity of PCR method for Escherichia coli O157:H7 was 1.7cfu. Enrichment with mTSB broth containing novobiocin gave good results compared to mEC broth with novobiocin by both PCR and cultural methods. [Vet. World 2010; 3(1.000): 13-16