Determination of Resistance Reference Parameters of Equine Strongyls to Anthelmintic

The research undertaken during November 2008 - April 2009 was aimed to find the required pharmacological reference parameters in order to diagnosis the resistance phenomenon of equine strongyls. Strongyls eggs originated from a total of 126 faecal samples collected during the 6 months of study on three different areas of the Danube Delta, where it is estimated that there are a number of over 4,000 wild untreated equine. Testing effectiveness of Mebendazole (MBZ), Fenbendazole (FBZ) and Ivermectine (IVM) was performed in vitro by larva development assay (LDA). To Fenbendazole the reference lethal concentrations were LC50 0.0089µg/ml; LC90 -0.7430µg/ml and LC100 -0.9265µg/ml with a MIC of -18.6031µg/ml. To Mebendazole the reference lethal concentrations were LC50 -0.0078µg/ml, LC90 -0.4566µg/ml and LC100 -0.5688µg/ml with a MIC of 21.4542µg/ml. To Ivermectine the reference parameters were LC50 -0.00028µg/ml, LC90 0.0011µg/ml and LC100 0.0013µg/ml with a MIC of -250.004µg/ml

Vascular abnormalities of the distal deep digital flexor tendon in 8 draught horses identified on histological examination

International audienceThe purpose of this study was to provide a detailed description of the vascular changes in the distal part of deep digital flexor tendon (DDFT). Eight isolated forelimbs were collected from 8 horses with DDF tendinopathy diagnosed post-mortem by ultrasound and gross anatomopathological examination. The samples were fixed in 10% neutral buffered formalin, softened in 4% phenol and dehydrated with ethylic alcohol. Goldner's Trichrome staining method was used. The histopathological examination revealed vascular proliferation associated with structural disorders of blood vessels. Angiogenesis, fibroplasia and consecutive hypertrophy of the vascular wall with or without vascular occlusion were the most common findings. Other histopathological findings were: endothelial cell edema, progressive metaplasia from squamous to cubic cells, vascular wall hyalinization, endothelial cells apoptosis/necrosis and endothelial desquamation. These results demonstrated damage of the distal deep digital flexor tendon vasculature which may progressively alter the structural integrity of the tendon and contribute to degenerative lesions. (c) 2013 Elsevier Ltd. All rights reserved