72 research outputs found
Cycling time trial performance is improved by carbohydrate ingestion during exercise regardless of a fed or fasted state
Antioxidant pathways are up-regulated during biological nitrogen fixation to prevent ROS-induced nitrogenase inhibition in Gluconacetobacter diazotrophicus
Gluconacetobacter diazotrophicus, an endophyte isolated from sugarcane, is a strict aerobe that fixates N2. This process is catalyzed by nitrogenase and requires copious amounts of ATP. Nitrogenase activity is extremely sensitive to inhibition by oxygen and reactive oxygen species (ROS). However, the elevated oxidative metabolic rates required to sustain biological nitrogen fixation (BNF) may favor an increased production of ROS. Here, we explored this paradox and observed that ROS levels are, in fact, decreased in nitrogen-fixing cells due to the up-regulation of transcript levels of six ROS-detoxifying genes. A cluster analyses based on common expression patterns revealed the existence of a stable cluster with 99.8% similarity made up of the genes encoding the α-subunit of nitrogenase Mo–Fe protein (nifD), superoxide dismutase (sodA) and catalase type E (katE). Finally, nitrogenase activity was inhibited in a dose-dependent manner by paraquat, a redox cycler that increases cellular ROS levels. Our data revealed that ROS can strongly inhibit nitrogenase activity, and G. diazotrophicus alters its redox metabolism during BNF by increasing antioxidant transcript levels resulting in a lower ROS generation. We suggest that careful controlled ROS production during this critical phase is an adaptive mechanism to allow nitrogen fixation
Secagem da madeira de louro preto (Nectandra cuspidata) em estufa de micro-ondas
Microwave drying will play an important role in the industrialization of timber in the near future, because improved dimensional stability and increased mechanical strength of wood are dependent on its drying. The objective of this study was to evaluate the drying of Louro preto wood using the microwave process and its influence on drying time, final moisture content, and formation of checks. A program originally developed for Mahogany wood was used due to the similarity between the basic density values of the two wood species. To this end, three (3) drying processes were conducted: drying S1, with initial average moisture content above the fiber saturation point (FSP), reached 13.99% of the final average moisture content after 2h 25min; whereas drying S2 and S3, with initial average moisture contents below the FSP, showed 14.00 and 14.62% of the final average moisture content after 2h 8min and 2h 2min, respectively. The drying processes were considered fast, and the difference in the initial moisture contents of the samples showed the influence of the treatments in the formation of end and surface checks. Drying S2 was more prone to present surface checks than end checks, whereas internal checks were not found in any of the drying processes performed
Quality of life of caregivers for patients of cerebrovascular accidents: association of (socio-demographic) characteristics and burden
Characterisation of DOG-1 expression in salivary gland tumours and comparison with myoepithelial markers
DOG1 is an established diagnostic marker for gastrointestinal stromal tumours (GIST), but has been reported in salivary gland tumours (SGT) as an acinar and intercalated duct marker. However, its specificity and distribution is not well established. The aim of this study was to evaluate the diagnostic utility of DOG-1 expression in SGT in addition to comparing it with myoepithelial markers. Normal salivary tissue and SGT (n = 184) were examined for expression of DOG1 and a range of myoepithelial markers. SGT included: acinic cell carcinoma (ACC, n = 15), secretory carcinoma (SC, n = 9), pleomorphic adenoma (PA, n = 49), carcinoma ex-PA (Ca ex-PA, n = 11), adenoid cystic carcinoma (AdCC, n = 20), polymorphous adenocarcinoma (PAC, n = 6), myoepithelioma (n = 6), myoepithelial carcinoma (MC, n = 2), basal cell adenoma (BCA, n = 14), canalicular adenoma (CA, n = 19), mucoepidermoid carcinoma (MEC, n = 11), oncocytoma (n = 2), adenocarcinoma NOS (AdNOS, n = 4), basal cell adenocarcinoma (BCAC, n = 2), salivary duct carcinoma (SDC, n = 3) and papillary cystadenocarcinoma (PCAC, n = 1). Normal acini and ACC (14/15) showed strong luminal DOG1 staining; SC were largely negative with only focal expression in 3/9 cases. Luminal staining was seen in PA (14/49), PAC (4/6), Ca ex-PA (4/11) and AdCC (6/20). 8/11 MEC showed luminal and/or mucous cell staining. No staining was seen in myoepithelioma, MC, CA, adNOS and BCAC. BCA showed strong staining of myoepithelial cells in some cases (5/14). Variable myoepithelial DOG1 staining was seen in PA, Ca ex PA, BCA, SDC and PCAC which was not as consistent as myoepithelial markers such as calponin, p63 and αSMA. Absence of DOG1 can differentiate ACC from SC, but staining is variable in PA, PLGA and Ca ex-PA. Myoepithelial staining in some tumours but not in normal gland suggests a wider distribution in SGT than originally envisaged
Produtividade e qualidade de frutos do meloeiro em função de tipos e doses de biofertilizantes
Ações de alimentação e nutrição na atenção básica: a experiência de organização no Governo Brasileiro
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