A functional analysis of cell cycle events in developing and germinating tomato seeds

Seeds are complex biological structures and the primary dispersal units of higher plants. They consist of nutrient reserve storage tissue(s), an embryo and encapsulating structures designated for protection and that may also regulate germination. Seeds have developed mechanisms of withstanding desiccation without losing the ability of immediate reactivation of embryo growth upon rehydration.The arrest and reactivation of cell cycle related events appear to be intimately linked to the arrest of growth during seed development and reactivation of growth during germination. In this thesis, DNA synthesis,β-tubulin accumulation and appearance of microtubular cytoskeleton are studied in relation to morphological and physiological events that are involved in seed development and germination. Biochemical techniques are used to study the relation between DNA replication andβ-tubulin accumulation in embryonic cells of germinating seeds (Chapters 2 and 3). Then, immunocytochemical techniques are applied for the detection and visualisation of DNA synthesis activity and appearance of microtubular cytoskeleton in seed tissues during development and in embryos during germination (Chapters 4, 5 and 6).The results obtained in this study show a general pattern of tomato seed development and germination based on the quantitation and distribution of nuclear DNA synthesis activity,β-tubulin accumulation and microtubular cytoskeleton appearance in cells of the seed tissues. The pattern in embryos during seed germination appeared as a "mirror image"of that during development (Chapters 4 and 5). Cell cycle activities are intense during early stages of seed development and then arrest as seeds become mature. Embryonic DNA synthesis activity, together with the presence of mitotic microtubular cytoskeleton arrays and cell divisions define the period of embryo histodifferentiation. Therafter, the presence of cortical microtubular cytoskeleton and absence of DNA synthesis acitvity defines the phase of embryo growth and completion of morphogenesis. Finally, the microtubular cytoskeleton is degraded as seeds become quiescent, defining the maturation phase. The roles of GA and ABA in seed development are evaluated in the differing developmental patterns observed for the GA-deficient ( gib1 ) and ABA-deficient ( sit w) mutants. Upon imbibition in water, the cell cycle is reactivated and becomes intense as germination is completed. Initially, the cortical microtubular cytoskeleton appears and DNA synthesis is initiated in meristematic cells of embryonic radicle tip region. Thereafter, replicated nuclear DNA is detected together with mitotic microtubular cytoskeleton arrays and cell divisions, prior to radicle protrusion through the seed coat. Both primary and secondary dormancy are characterised by a very low cell cycle activity. The depth of dormancy appears to be related to the progression of the cell cycle prior to the induction of dormancy (Chapter 6).Finally, in the form of a descriptive model, it is proposed to modify the current notion of the different phases in tomato seed development and germination.</p

Guidelines on the treatment of anemia of chronic renal failure using recombinant human erythropoietin: associação brasileira de hematologia, hemoterapia e terapia celular guidelines project: Associação médica brasileira - 2014

The guidelines project is a joint initiative of the Associação Médica Brasileira and the Conselho Federal de Medicina. It aims to collect information to standardize decisions and help create strategies during diagnosis and treatment. These data were prepa366450453sem informaçãosem informaçã

Top-down structures of mafic enclaves within the Valle Fértil magmatic complex (Early Ordovician, San Juan, Argentina)

Magmatic structures related to the mechanical interaction between mafic magmas and granitoids have been studied in the Valle Fértil calc-alkaline igneous complex, Argentina. Excepcional outcrops with vertical walls of more than 300 m high allow us the study of three-dimensional geometries of individual blobs of mafic magma as well as the geometry of pipe-like structures in which mafic microgranular enclaves are concentrated in more than 50 times the normal abundance in the granodiorite mass. The shape of enclaves and pipe-like structures are interpreted as the ressult of top-to-down intrusions of a mafic magma into a granodiorite-tonalite mass. These sinking structures are the result of a reverselly stratified magma chamber with gabbros and diorites at the top and granodiorite-tonalite at the bottom. They may account for most of the structures found in microgranular enclaves and magma mingling zones that characterize calc-alkaline batholiths. Synplutonic intrusions from the top is the only plausible mechanism to account for the observed structures. The model may be of general application to calc-alkaline batholiths characterized by the presence of mafic microgranular enclaves. An implication of these reverselly stratified magma chambers is the presence of a petrological inversion which may be the consequence of cold diapirs emplaced below the mantle wedge in a suprasubduction setting

Depth of dormancy in tomato (Lycopersicon esculentum Mill.) seeds is related to the progression of the cell cycle prior to the induction of dormancy

Cell cycle activities are initiated following imbibition of non-dormant seeds. However, it is not known whether cell cycle related events other than DNA replication also remain suppressed in imbibed dormant seeds. The objective of this study was to demonstrate that the transitions between the non-dormant and dormant (both primary and secondary) states are reflected in cell cycle events, such as DNA replication and the changing patterns of the microtubular cytoskeleton involved in the processes of growth and development. The present studies were conducted on seeds from tomato (Lycopersicon esculentum cv. Moneymaker) that possessed primary dormancy or were manipulated to attain secondary dormancy. In addition, a non-dormant abscisic acid (ABA)-deficient mutant, sitw, was used. DNA replication, as measured by flow cytometry, and -tubulin accumulation, analysed by immunoblotting, were compared with immunocytological studies of active DNA synthesis and microtubular cytoskeleton formation. It is shown that the depth of dormancy, which distinguishes primary and secondary dormancy, may depend on the progression of the cell cycle prior to the induction of dormancy

Relativistic graphene ratchet on semidisk Galton board

Using extensive Monte Carlo simulations we study numerically and analytically a photogalvanic effect, or ratchet, of directed electron transport induced by a microwave radiation on a semidisk Galton board of antidots in graphene. A comparison between usual two-dimensional electron gas (2DEG) and electrons in graphene shows that ratchet currents are comparable at very low temperatures. However, a large mean free path in graphene should allow to have a strong ratchet transport at room temperatures. Also in graphene the ratchet transport emerges even for unpolarized radiation. These properties open promising possibilities for room temperature graphene based sensitive photogalvanic detectors of microwave and terahertz radiation.Comment: 4 pages, 4 figures. Research done at Quantware http://www.quantware.ups-tlse.fr/. More detailed analysis is give

Computational Physics on Graphics Processing Units

The use of graphics processing units for scientific computations is an emerging strategy that can significantly speed up various different algorithms. In this review, we discuss advances made in the field of computational physics, focusing on classical molecular dynamics, and on quantum simulations for electronic structure calculations using the density functional theory, wave function techniques, and quantum field theory.Comment: Proceedings of the 11th International Conference, PARA 2012, Helsinki, Finland, June 10-13, 201