Influence of culture media on the physical and chemical properties of Ag–TiCN coatings

The aim of this study was to verify the possible physical and chemical changes that may occur on the surface of Ag–TiCN coatings after exposure to the culture media used in microbiological and cytotoxic assays, respectively tryptic soy broth (TSB) and Dulbecco's modified eagle's medium (DMEM). After sample immersion for 24 h in the media, analyses were performed by glow discharge optical emission spectroscopy discharge radiation (GDOES), Rutherford backscattering spectroscopy (RBS) and x-ray photoelectron spectroscopy (XPS). The results of GDOES profile, RBS and XPS spectra, of samples immersed in TSB, demonstrated the formation of a thin layer of carbon, oxygen and nitrogen that could be due to the presence of proteins in TSB. After 24 h of immersion in DMEM, the results showed the formation of a thin layer of calcium phosphates on the surface, since the coatings displayed a highly oxidized surface in which calcium and phosphorus were detected. All these results suggested that the formation of a layer on the coating surface prevented the release of silver ions in concentrations that allow antibacterial activity.IC acknowledges the financial support of FCT-Fundacao para a Ciencia e a Tecnologia through the grant SFRH/BD/67022/2009. REG acknowledges support from Ramon y Cajal programme (RyC2007-0026). This research is sponsored by FEDER funds through the program COMPETE-Programa Operacional Factores de Competitividade and by national funds through FCT-Fundacao para a Ciencia e a Tecnologia in the framework of the Strategic Projects PESTC/FIS/UI607/2011, PEST-C/EME/UI0285/2011, PTDC/CTM/102853/2008.The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project 'BioHealth-Biotechnology and Bioengineering approaches to improve health quality', Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER. The authors also thank the project 'Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB', Ref. FCOMP-01-0124-FEDER-027462

Protocolo para determinação de açúcares totais em hortaliças pelo método de DNS.

bitstream/item/81580/1/cot-85.pd

Protocolo para determinação de açúcares redutores pelo método de Somogyi-Nelson.

bitstream/item/81814/1/cot-86.pd

Produção de batata pré-frita congelada.

bitstream/item/81816/1/cot-87.pd

Self-organized patterns of coexistence out of a predator-prey cellular automaton

We present a stochastic approach to modeling the dynamics of coexistence of prey and predator populations. It is assumed that the space of coexistence is explicitly subdivided in a grid of cells. Each cell can be occupied by only one individual of each species or can be empty. The system evolves in time according to a probabilistic cellular automaton composed by a set of local rules which describe interactions between species individuals and mimic the process of birth, death and predation. By performing computational simulations, we found that, depending on the values of the parameters of the model, the following states can be reached: a prey absorbing state and active states of two types. In one of them both species coexist in a stationary regime with population densities constant in time. The other kind of active state is characterized by local coupled time oscillations of prey and predator populations. We focus on the self-organized structures arising from spatio-temporal dynamics of the coexistence. We identify distinct spatial patterns of prey and predators and verify that they are intimally connected to the time coexistence behavior of the species. The occurrence of a prey percolating cluster on the spatial patterns of the active states is also examined.Comment: 19 pages, 11 figure

Dating COINS: Kinematic Ages for Compact Symmetric Objects

We present multi-epoch VLBA observations of Compact Symmetric Objects (CSOs) from the COINS sample (CSOs Observed In the Northern Sky). These observations allow us to make estimates of, or place limits on, the kinematic ages for those sources with well-identified hot spots. This study significantly increases the number of CSOs with well-determined ages or limits. The age distribution is found to be sharply peaked under 500 years, suggesting that many CSOs die young, or are episodic in nature, and very few survive to evolve into FR II sources like Cygnus A. Jet components are found to have higher velocities than hot spots which is consistent with their movement down cleared channels. We also report on the first detections of significant polarization in two CSOs, J0000+4054 (2.1%) and J1826+1831 (8.8%). In both cases the polarized emission is found in jet components on the stronger side of the center of activity.Comment: 34 pages including 7 figures, Accepted to ApJ on Dec 7, 200

Polarimetry of Compact Symmetric Objects

We present multi-frequency VLBA observations of two polarized Compact Symmetric Objects (CSOs), J0000+4054 and J1826+1831, and a polarized CSO candidate, J1915+6548. Using the wavelength-squared dependence of Faraday rotation, we obtained rotation measures (RMs) of -180 \pm 10 rad m^-2 and 1540 \pm 7 rad m^-2 for the latter two sources. These are lower than what is expected of CSOs (several 1000 rad m^-2) and, depending on the path length of the Faraday screens, require magnetic fields from 0.03 to 6 \mu G. These CSOs may be more heavily affected by Doppler boosting than their unpolarized counterparts, suggesting that a jet-axis orientation more inclined towards the line of sight is necessary to detect any polarization. This allows for low RMs if the polarized components are oriented away from the depolarizing circumnuclear torus. These observations also add a fourth epoch to the proper motion studies of J0000+4054 and J1826+1831, constraining their kinematic age estimates to >610 yrs and 2600 \pm 490 yrs, respectively. The morphology, spectrum, and component motions of J1915+6548 are discussed in light of its new classification as a CSO candidate, and its angle to the line of sight (~50\deg) is determined from relativistic beaming arguments.Comment: 29 pages, including 9 figures; Accepted by Astrophysical Journal, 16 Feb 0

Disponibilidade de ferro e zinco em feijão comum para estratégia de biofortificação.

Este trabalho teve como objetivo determinar os níveis de fósforo, ferro e zinco em grãos de feijão de diferentes cultivares, nas formas crus e cozidos, para verificar a variabilidade e o efeito do cozimento na retenção mineral.CONAF