Study of Leishmania pathogenesis in mice : experimental considerations

Although leishmaniases are endemic in 98 countries, they are still considered neglected tropical diseases. Leishmaniases are characterized by the emergence of new virulent and asymptomatic strains of Leishmania spp. and, as a consequence, by a very diverse clinical spectrum. To fight more efficiently these parasites, the mechanisms of host defense and of parasite virulence need to be thoroughly investigated. To this aim, animal models are widely used. However, the results obtained with these models are influenced by several experimental parameters, such as the mouse genetic background, parasite genotype, inoculation route/infection site, parasite dose and phlebotome saliva. In this review, we propose an update on their influence in the two main clinical forms of the disease: cutaneous and visceral leishmaniases

Physiological quality of seeds of crambe desiccated at pre-harvest with glyphosate

ABSTRACT Early harvest may decrease seed exposure in the field after physiological maturation. The objective of this study was to evaluate the effect of glyphosate desiccant at different plant desiccation times at pre-harvest on the physiological quality of crambe (Crambe abyssinica Hochst) seeds. The experimental design was completely randomized in a 2 x 4 factorial scheme, involving the application of glyphosate desiccant in the plants at pre-harvest and the control (without plant desiccation) and four desiccation times, with three replicates per treatment. The desiccation of crambe plants at pre-harvest with glyphosate was efficient, positively affecting seed germination and vigor. Application of the desiccant in the plants, with 90% of brown seeds, at 103 days after sowing, allows the production of seeds with superior performance

Physiological quality of seeds of crambe desiccated at pre-harvest with glyphosate

<div><p>ABSTRACT Early harvest may decrease seed exposure in the field after physiological maturation. The objective of this study was to evaluate the effect of glyphosate desiccant at different plant desiccation times at pre-harvest on the physiological quality of crambe (Crambe abyssinica Hochst) seeds. The experimental design was completely randomized in a 2 x 4 factorial scheme, involving the application of glyphosate desiccant in the plants at pre-harvest and the control (without plant desiccation) and four desiccation times, with three replicates per treatment. The desiccation of crambe plants at pre-harvest with glyphosate was efficient, positively affecting seed germination and vigor. Application of the desiccant in the plants, with 90% of brown seeds, at 103 days after sowing, allows the production of seeds with superior performance.</p></div