Garlic (Allium spp.) viruses: detection, distribution and remediation attempts in a European garlic collection

Garlic is an important vegetable crop in numerous countries used as food and natural based medicine. Similar to the majority of vegetatively propagated plants, garlic may be affected by several viruses that can cause severe crop losses. The present study aimed to screen 105 garlic accessions (mother plants) from 5 European countries (Germany, Czech Republic, Poland, Italy, and France) for possible presence of Onion yellow dwarf virus (OYDV), Leek yellow stripe virus (LYSV), Garlic common latent virus (GCLV) and Shallot latent virus (SLV). The occurrence of three Allexiviruses (GarV-A, GarV-B and GarV-C) in mixed assays was also investigated. Meristem-tip culture assays were performed in order to attempt eradication of the studied viruses. Garlic viruses identification was made by ELISA and RT-PCR. ELISA outcomes showed that all 105 garlic accessions were infected by different virus combinations. The OYDV and LYSV were identified, by ELISA, in all countries at 96% and 88,6% respectively and by RT-PCR at 99% and 96%. Furthermore, GCLV and SLV were detected by ELISA in about 88% and by RT-PCR at 89% and 90%, respectively with the exception of the studied Allexiviruses which were not amplified by RT-PCR with ALLEX1/ALLEX2 primers. Smaller meristem size (0,3-1,5 mm) led to better virus elimination efficiency (29%) compared to 8% obtained for the larger size (2-2,5 mm). The outcomes were opposite (16% vs. 90%) for plants regeneration. Virus elimination efficiency was linked to the virus type, e.g., OYDV and LYSV were eradicated at 90% while GCLV and Allexiviruses were difficult to eliminate (57,4% and 55,6% of eradication). Given the economic relevance of garlic crops worldwide and the frequently reported incidence of viral infections, it is important to make virusfree germplasm available. Therefore, investigating the garlic germplasm sanitary status and constantly improving it is of crucial importance aiming to increase the overall garlic production

Quality Traits of Some Cauliflower Cultivars Grown in the “Valle dell’Ofanto” Area (Italy) as Affected by Post-Harvest Storage

Some quality characteristics of the “Valle dell’Ofanto” cauliflower, that has been recently appointed the certification of collective brand, were investigated at Gaudiano (41°03’N; 15°42’E, Southern Italy, Basilicata Region). Six white head cauli-flower cultivars, characterized by a different length of the crop cycle (2 early, 2 medium and 2 late), were studied. The above cultivars were transplanted in open field at the middle of August 2004 and harvested from the middle of October 2004 to the end of March 2005. At harvest time, yield traits and head sizes of cauliflowers were measured. Among the qualitative traits, color, weight loss, total soluble solids, nitrate and vitamin C content were assessed on the fresh florets and after a storage period lasting 15 days at 0°C and 95% R.H. The “Valle dell’Ofanto” cauliflower was charac¬terized by a high content in sugars and vitamin C, even if significant differences in some qualitative parameters (e.g., vitamin C and nitrate content) were affected by cultivars and crop cycle length. On the other hand, the storage at controlled tempera¬ture and R.H. did not substantially change the qualitative characteristics of the heads, but caused only a slight increase of dry matter and soluble solids levels, especially in those cultivars more susceptible to weight loss

Does soil thinning change soil erodibility? An exploration of long-term erosion feedback systems

Soil erosion rates on arable land frequently exceed the pace at which new soil is formed. This imbalance leads to soil thinning (i.e. truncation), whereby subsoil horizons and their underlying parent material become progressively closer to the land surface. As soil erosion is a selective process and subsurface horizons often have contrasting properties to the original topsoil, truncation-induced changes to soil properties might affect erosion rates and runoff formation through a soil erosion feedback system. However, the potential interactions between soil erosion and soil truncation are poorly understood due to a lack of empirical data and the neglection of long-term erodibility dynamics in erosion simulation models. Here, we present a novel model-based exploration of the soil erosion feedback system over a period of 500 years using measured soil properties from a diversified database of 265 agricultural soil profiles in the UK. For this, we adapted the Modified Morgan–Morgan–Finney model (MMMF) to perform a modelling experiment in which topography, climate, land cover, and crop management parameters were held constant throughout the simulation period. As selective soil erosion processes removed topsoil layers, the model gradually mixed subsurface soil horizons into a 0.2 m plough layer and updated soil properties using mass-balance mixing models. Further, we estimated the uncertainty in model simulations with a forward error assessment. We found that modelled erosion rates in 99 % of the soil profiles were sensitive to truncation-induced changes in soil properties. The soil losses in all except one of the truncation-sensitive profiles displayed a decelerating trend, which depicted an exponential decay in erosion rates over the simulation period. This was largely explained by decreasing silt contents in the soil surface due to selective removal of this more erodible particle size fraction and the presence of clayey or sandy substrata. Moreover, the soil profiles displayed an increased residual stone cover, which armoured the land surface and reduced soil detachment. Contrastingly, the soils with siltier subsurface horizons continuously replenished the plough layer with readily erodible material, which prevented the decline of soil loss rates over time. Although our results are limited by the edaphoclimatic conditions represented in our data, as by our modelling assumptions, we have demonstrated how modelled soil losses can be sensitive to erosion-induced changes in soil properties. These findings are likely to affect how we calculate soil lifespans and make long-term projections of land degradation

Blending academic and professional learning in a university course for future e-learning specialists: The perspective of company tutors

Blended learning usually refers to the combination of online/offline instructional methods. In this paper, we describe a university course in “E-learning Psychology” designed to blend not only modes of teaching, tools, and media, but also learning contexts; specifically, academic and professional contexts. To achieve an effective blend of learning contexts, students were monitored by academic and company tutors through an instant messaging app (WhatsApp). The unique contribution of the company tutor to the blending of academic and professional contexts is explored. By qualitatively analyzing (i) process data (four WhatsApp log chats) and (ii) self-report data (interviews with six company tutors), we found that the company tutor contributed to both the traditional blended dimension (mixing online and offline) and to the blend of the academic and professional contexts. When company tutors participated in the chat, students moved from an organizational dynamic, featuring chats monitored by only the academic tutor, toward a more collaborative and reflective dynamic. The company tutors considered the opportunity to blend academic and professional contexts as the best aspect of the course for both themselves as educators/company representatives, and for the students. This paper offers insights into the ongoing discussion about what blended is—or should be—and the role of company tutors in blending educational contexts

Relationship between chemical composition and nematicidal activity of different essential oils

In this study, the relationship between nematicidal activity and chemical composition of ten essential oils (EOs) from different plant species was investigated both in in vitro assays on juveniles (J2) and eggs of the root-knot nematode Meloidogyne incognita and in experiments on tomato in soil infested by M. incognita. Nematode J2 were exposed for 4, 8 or 24 h to 0.78–100 µg mL−1 concentrations of each EO, whereas 24, 48 or 96 h exposures to 250, 500 and 1000 µg mL−1 solutions were tested on M. incognita egg masses. Treatments with 50, 100 or 200 µg kg soil rates of each EO were applied in the experiment on potted tomato. The highest nematicidal potential resulted for the C. verum EO, as highly toxic to both M. incognita J2 and eggs and strongly suppressive on nematode multiplication on tomato roots. The infestation of M. incognita on tomato roots was also strongly reduced by the EOs from E. citriodora and S. aromaticum, both highly toxic to M. incognita J2 but less active on nematode eggs. Adversely, R. graveolens EO strongly inhibited the egg hatch but was limitedly toxic to the infective J2. Chemical composition of the EOs was determined by GC-FID and GC-MS. The ten EOs showed a very different chemical composition in terms of major phytochemicals, with one or two dominant components totally amounting up to 85%. The structure–activity relationship based on the main phytochemicals identified in the assayed EOs and their nematicidal effects on M. incognita was also discussed. Results from this study confirmed that the selection of suitable EO raw materials can lead to the formulation on new effective nematicidal products

Avaliacao genetica e selecao em Eucalyptus grandis na regiao de Arapoti - PR.

Foram avaliados 17.916 indivíduos, pertencentes a 163 progênies de polinização aberta de 11 populações de Eucalyptus grandis, implantadas em duas fazendas na região de Arapoti - PR, visando a seleção de indivíduos com base em seus valores genéticos preditos. Foram obtidas estimativas médias da herdabilidade no sentido restrito, em nível de indivíduos e da acurácia seletiva, da ordem de 40 e 65%, respectivamente. A estimativa (0,75) da correlação genética entre médias de progênies, nas duas fazendas, indicou que a interação genótipo X ambiente não é problemática para o melhorista. Ganhos genéticos da ordem de 87 a 105% poderão ser obtidos com o estabelecimento das populações de melhoramento e de produção de sementes, respectivamente

Perfectly Translating Lattices on a Cylinder

We perform molecular dynamics simulations on an interacting electron gas confined to a cylindrical surface and subject to a radial magnetic field and the field of the positive background. In order to study the system at lowest energy states that still carry a current, initial configurations are obtained by a special quenching procedure. We observe the formation of a steady state in which the entire electron-lattice cycles with a common uniform velocity. Certain runs show an intermediate instability leading to lattice rearrangements. A Hall resistance can be defined and depends linearly on the magnetic field with an anomalous coefficient reflecting the manybody contributions peculiar to two dimensions.Comment: 13 pages, 5 figure

Eye fixations to figures in a four-choice situation with luminance balanced areas: Evaluating practice effects

Contingency analyses of eye movements may reveal variables that are relevant to the stimulus control of observing behavior. The present research tracked the eye movements of four adults exposed to a simultaneous discrimination among four stimuli, two two-dimensional (square and circle) and two three-dimensional (cube and cylinder) mono-chromatic figures with approximately equal luminance. On each discrimination trial, the stimuli were displayed in the four corners of a video monitor and participants chose among them by pressing corresponding keys. For two participants, choosing either cube or square (S+) was followed by the word “correct” and a 3-second inter-trial interval. Alternatively, choosing either cylinder or circle (S-) was followed by "incorrect" and a 30-second inter-trial interval. For the other two participants, contingencies were reversed. The position of the stimuli on the screen varied randomly across trials. The procedure continued for 80 trials. During these trials, discriminated choices were established. Despite the presence of both a two- and a three-dimensional S+ on each trial, responses to the three-dimensional S+ tended to prevail. Although general eye fixations tended to decrease as discrimination was established, subjects tended to observe S+ for longer durations than S-. Characteristics of the stimuli may interact with the contingencies of reinforcement in the stimulus control of observing behavior

Crocus sativus L. Ecotypes from Mediterranean Countries: Phenological, Morpho-Productive, Qualitative and Genetic Traits

The characterization of C. sativus ecotypes is of great interest for preserving them from a possible genetic erosion due to the decrease of European cultivation surface. In this study, we evaluated four ecotypes from Italy (Sardinia and Abruzzo), Spain (Castilla-La Mancha), and Greece (Kozani) in order to detect the existence of variability and promote the biodiversity of this crop. Thirty-one traits related to saffron flowering, flower morphology, production of spice and daughter corms, vegetative development (leaf and corm traits), and spice quality, were evaluated. In addition, a genetic analysis through three PCR-based approaches, SSRs, RAPD, and SRAP was assessed. Results highlighted a phenotypic variation among ecotypes during two consecutive years. All the studied parameters were influenced by the ecotype except for the stamen length, color coordinates of tepals, leaf length, and leaf number per plant. Sardinia had a longer flowering interval, earlier flowering, and higher spice yield and quality than the other corm origins. The maximum values of morphological traits, such as stigma length, dry weight of stigmas, tepals, flowers and leaves, leaf area, and daughter corm weight were observed in the Abruzzo ecotype. Principal component analysis (PCA) showed a clear separation among ecotypes, in which Sardinia and Spain showed more similarities than Abruzzo and Kozani. Significant negative correlation was found between days to flower with stigma yield and quality. However, we could not find molecular markers discriminating among corm origins. In conclusion, this study suggests the importance of C. sativus ecotypes as precious source of biodiversity and bioactive compounds, and of their enhancement as fundamental prerequisite for a sustainable development strategy and as an agricultural diversification opportunity for growers