Rola krążącego sTWEAK w patogenezie choroby Hashimoto — badanie pilotażowe

  Introduction: We aimed to investigate the role of sTWEAK in the pathogenesis of Hashimoto’s thyroiditis, which is a chronic inflammatory autoimmune disease. Material and methods: A total of 80 patients were included in the study, 60 of whom were newly diagnosed with Hashimoto’s thyroiditis (20 patients in each of the euthyroid, subclinical hypothyroid, and overt hypothyroid subgroups), and 20 of whom were healthy volunteers. Thyroid function tests and autoantibodies were measured using the electro-chemiluminescence immunoassay method, and sTWEAK, IL-17A, IL-12, and TGF-beta1 were measured using enzyme-linked immunosorbent assay method. Results: The Hashimoto’s Thyroiditis group had lower levels of sTWEAK and TGF-beta1, but had higher levels of IL-12 and IL-17A as compared to the control group. Of these, only the difference between IL-17A levels reached statistical significance (2.1 pg/mL vs. 1.8 pg/mL, respectively; p < 0.001). While the levels of sTWEAK were similar in the control, euthyroid, and subclinical groups, the overt hypothyroidism group had lower level of sTWEAK than that of subclinical hypothyroidism (687.6 ± 153.3 pg/mL vs. 888.2 ± 374.4 pg/mL, respectively; p = 0.03). A negative correlation was determined between sTWEAK level and anti-TPO (r = –0.533, p = 0.028) and IL-17A (r = –0.600, p = 0.005) levels in the overt hypothyroidism group. Conclusions: The reduced levels of sTWEAK with progression of Hashimoto’s Thyroiditis and the significant correlation between the sTWEAK levels and anti-TPO found in this study suggest that sTWEAK plays an active role in chronic inflammation in the pathogenesis of Hashimoto’s Thyroiditis and in the progression of autoimmunity. (Endokrynol Pol 2016; 67 (6): 562–566)    Wstęp: Badanie przeprowadzono w celu ustalenia roli sTWEAK w patogenezie zapalenia tarczycy Hashimoto, przewlekłej zapalnej choroby autoimmunologicznej. Materiał i metody: Do badania włączono łącznie 80 chorych, w tym 60 osób z nowo rozpoznaną chorobą Hashimoto (po 20 chorych w podgrupach z eutyreozą, subkliniczną niedoczynnością tarczycy i jawną niedoczynnością tarczycy) i 20 zdrowych ochotników. Badania czynności tarczycy oraz oznaczenia stężenia autoprzeciwciał przeprowadzono przy użyciu metod elektrochemiluminescencji, a stężenia sTWEAK, IL-17A, IL-12 i TGF-beta1 oznaczono za pomocą testów enzymatycznych. Wyniki: W grupie osób z chorobą Hashimoto stężenia sTWEAK i TGF-beta1 były niższe, a stężenia IL-12 i IL-17A wyższe niż w grupie kontrolnej. Jednak tylko różnice między stężeniami IL-17A osiągnęły poziom istotności statystycznej (odpowiednio 2,1 pg/ml vs. 1,8 pg/ml; p < 0,001). Podczas gdy stężenia sTWEAK były podobne w grupach kontrolnej, z eutyreozą i z subkliniczną niedoczynnością tarczycy, stężenia sTWEAK w grupie z jawną niedoczynnością tarczycy były niższe niż u osób z subkliniczną niedoczynnością tarczycy (odpowiednio 687,6 ± 153,3 pg/ml vs. 888,2 ± 374,4 pg/ml; p = 0,03). Stwierdzono ujemną korelację między stężeniem sTWEAK a stężeniami przeciwciał przeciw TPO (r = –0,533; p = 0,028) oraz IL-17A (r = –0,600; p = 0.005) w grupie z jawną niedoczynnością tarczycy. Wnioski: Obniżanie się stężenia sTWEAK z progresją choroby Hashimoto oraz istotna korelacja między stężeniem sTWEAK a stężeniem przeciwciał przeciw TPO stwierdzone w tym badaniu wskazują, że sTWEAK odgrywa aktywną rolę w przewlekłym zapaleniu w patogenezie choroby Hashimoto, a także w progresji autoagresji. (Endokrynol Pol 2016; 67 (6): 562–566)

Biochemical Characterisation and Sensory Evaluation of Differently Coloured and Shaped Tomato Cultivars

Antioxidant capacity, total phenolic content, colour, sugar, volatiles, ascorbic acid and carotenoid (β-carotene and lycopene) contents of differently coloured and shaped tomato cultivars (cvs) grown in the Eastern Mediterranean region, Turkey were determined, along with a sensory evaluation. Tomato cultivars of two different types (cherry and beefsteak) and four different colours (red, yellow, orange and brown) were analysed. All plants were simultaneously grown in the same field and subjected to identical horticultural practices to minimise the effects of environmental conditions and to maximise those related to genotype. The red cherry cultivar had the highest lycopene content, while the orange beefsteak cultivar had the highest β-carotene content. The highest antioxidant capacity, total phenolic content and hardness scores were found in cherry-type tomatoes, except the yellow one. The red cherry cultivar had the highest sugar content. Red and brown cherry cultivars were also significant in terms of their high carotenoid and sugar contents, along with a high antioxidant activity. The brown cherry cultivar had the highest total phenol content. The highest quantities of 2-hexenal, 3-hexen-1-ol, and 6-methyl-5-hepten-2-one were detected in red cultivars. The brown cherry cultivar had the highest sweetness, typical aroma and hardness scores, while the yellow beefsteak cultivars the lowest sweetness typical aroma scores. In terms of sensory parameters, red and brown cultivars scored higher than yellow and orange ones

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Effects on shelf life and fruit quality and physiology of different postharvest treatments in postharvest life of bananas where growing in Anamur.

TEZ8809Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2012.Kaynakça (s. 157-162) var.xiii, 171 s. : res. ; 29 cm.Postharvest quality and quantity of Dwarf Cavendish and Grand Nain bananas, which is known as ""Anamur bananas"", have economically important in Turkey. Bananas gives different responses to postharvest applications due to their different physiologies from many products. For a successful storage, different postharvest applications should be applied in different physiological stages. In this study, 1-Methylcyclopropene (1-MCP), that is well used in postharvest of many products, modified atmosphere packaging (MAP) and potassium permanganate applications and their combinations were used in green and in level 3 maturated banana. All shelf life analysis had been carried out after 8 days of storage applications to maturated bananas and 40 days of storage applications to gren bananas and sensory analyses were done for target customers. The research results showed that using MAP alone and with other combinations at maturity level 3 were decreased weight losses in half (Control %5.20 , MAP %2.99). It was found that MAP only application was also effective on postharvest losses (MAP %12.67 Control %24.13). Using 1-MCP at maturity level 3 was not economic. It was also seen that using 1-MCP only application in gren bananas was abnormally increased yellow and green sharp contrasts. In gren bananas, it was examined that, using MAP only and MAP with other applications were importantly decreased the weight losses (MAP %3.50, Control %8.08). It was sufficient to use 1000 ppm imazalil in order to decrease postharvest losses.Ülkemizde yetiştirilen ve Anamur muzu olarak bilinen muzlardan 'Dwarf Cavendish' ve 'Grand Nain' çeşitlerinin derim sonrası fizyolojilerinin bilinmesi bu ürünlerin muhafazası açısından oldukça önemlidir. Muzlar bir çok üründen farklı fizyolojileri nedeni ile derim sonrası uygulamalara da farklı tepkiler vermektedir. Bu nedenle başarılı muhafaza için farklı derim sonrası uygulamaların farklı fizyolojik evrelerde kullanımları önem arzetmektedir. Bu çalışmada derim sonrası birçok üründe başarılı bir şekilde kullanılan 1-Methylcyclopropene, modifiye atmosfer paketi (MAP) ve potasyum permanganat uygulamaları ile bunların kombinasyonları yeşil ve 3 no'lu olgunluk aşamalarındaki muzlar üzerine etkileri belirlenmiştir. Uygulamalar yapıldıktan sonra olgunlaştırılan muzlarda 8 gün, yeşil muzlarda 40. güne kadar muhafazadan sonra 8 günlük raf ömrü süresince laboratuar analizleri yapılmış, hedef kitleye yönelik olarak duyusal paneller yürütülmüştür. Araştırma sonuçları 3 no'lu olgunluk aşamasındaki muzlarda MAP'ı tek başına veya bir başka uygulama ile birlikte kullanmanın ağırlık kayıplarını yaklaşık olarak yarı yarıya azalttığını göstermiştir (kontrol %5.20, MAP %2.99). MAP tek başına kullanıldığında derim sonrası kayıpları üzerine de etkili bulunmuştur (MAP %12.67, kontrol %24.13) 1-MCP'nin halihazırda 3 no'lu olgunluk aşamasındaki muzlarda kullanımı ekonomik değildir, yeşil muzlarda tek olarak uygulandığında sarı ve yeşil kontrastını normaldışı artırarak renklenme bozuklukları oluşturduğu görülmüştür. Yeşil muzlarda da MAP uygulaması tek başına ve ombinasyonlarda kullanıldığında ağırlık kayıplarını önemli ölçüde azalttığı tespit edilmiştir (MAP %3.50, kontrol %8.08). 1000ppm imazalil kullanımı derim sonrası kayıpları azaltmak için yeterlidir

Mersin ili yaylalarında açılan doğal depoları limon muhafazasında kullanılanım olanaklarının araştırılması

TEZ5128Tez (Yüksek Lisans) -- Çukurova Üniversitesi, Adana, 2004.Kaynakça (s. 108-113) var.x, 127 s. ; rnk. res. ; 30 cm.

Important points for succesful lemon storage in natural cold storages

Bu çalışmanın temelini 2002-2003 yılları arasında-yürütmüş olduğumuz Ortahisar ve Mersin yayla depolarında limon muhafazası ile ilgili proje oluşturmuştur. Bu makalede proje yürütülürken limon muhafazası ile ilgili karşılaştığımız veya eksikliğini gördüğümüz bazı noktalara değinmek ve daha önceki araştırmacıların limon muhafazası için dikkat edilmesi gereken noktalarla ilgili yorumlarını belirtmek sureti ile pratik bir başvuru kaynağı oluşturulması hedeflenmiştir. Ülkemizde limon da derim sonrası kayıplar ortalama her yıl için 10 ila 24 milyon $arasında bir rakama ulaşmaktadır. Derim sonrası kayıpları azaltmak için depolanacak limonun yetiştirildiği bahçelerden başlayarak satış aşamasına kadar dikkat edilmesi gereken önemli noktalar vardır. Limon ' meyvesinin yetiştirildiği ağacın beslenme durumu, bahçe ilaçlamaları, derim zamanı, derim şekli, ambalajlama, deponun yapısı ve ambalajların depodaki dizilişi ile depo içerisinin havalandırılması başarılı bir limon muhafazası için dikkat edilmesi gereken önemli noktalardandır.Basic of this study was constituted the project about lemon storage in natural storages where Ortahisar and Plato of Mersin and was carried out by us between the years of 2002 and 2003. In this paper, it was noticed that was faced or seen lack of some important points about lemon storage when project was been doing and it was aimed to form a practical source of application as to show comments of the preceding researchers about important points of lemon storage. In Turkey, the value of postharvest losses of lemon has reached about 10-24 million$ for each year. There are important points should take note to reducing postharvest losses. Nutritional status of lemon three, spraying program of the orchard, harvest time, harvest method, boxing, structure of the storage house, arrangement of boxes in storage houses and ventilation are important points that should be taken in consideration for successful lemon storage

Nar (Punica granatum L.) Çeşit ve Genotiplerin Fizikokimyasal Karakterizasyonu

Bu çalışmada; Türkiye’de yetiştirilen standart nar çeşitleri (Hicaz narı, Silifke Aşısı, Katırbaşı, 33N23-Çevlik, 01N04-Fellahyemez, 33N34, İzmir-26, İzmir-23, İzmir-1513, 33N24, Kuş narı ) ile Gündoğdu (2006)’nun Pervari (Siirt) yöresinde daha önceden belirlemiş olduğu en iyi 5 nar genotipinde (56PER021, 56PER022, 56PER020, 56PER019, 56PER003) meyvelerin pomolojik özellikleri ve bazı kimyasal içeriklerinin tespit edilmesi amaçlanmıştır. Araştırmada meyve ağırlığını 251.01-530.25 gr, meyve uzunluğunu 60.30-89.97 mm, meyve enin 75.57- 100.68 mm, meyve hacminin 230.00-542.50 cm3, meyve suyu miktarının 106.66-186 ml ve meyve yoğunluğunun 0.92 -1.19 gr cm-3, SÇKM miktarının % 11.50-14.62, pH 3.45-4.71, şekil indeksinin 0.82-0.92 ve toplam asitliğin % 0.19-1.17 arasında değişiklik gösterdiği tespit edilmiştir. Bunun yanında incelenen çeşit ve genotiplerin kabuk alt zemin rengi, üst zemin rengi, çekirdek sertliği, meyve tadı, dane rengi, üst odacık sayısı, alt odacık sayısı, odacıkların görünümü ve daneleme kolaylığı belirlenmiştir