Contribution to increase the usefulness of the present differential series for common bean angular leaf spot.

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Population structure and genetic diversity of maize inbreds derived from tropical hybrids

The objectives of this study were to identify the population structure and to assess the genetic diversity of maize inbreds. We genotyped 81 microsatellite loci of 90 maize inbreds that were derived from tropical hybrids and populations. The population structure analysis was based on a Bayesian approach. Each subpopulation was characterized for the effective number of alleles, gene diversity, and number of private alleles. We also performed an analysis of molecular variance and computed a measure of population differentiation (FST). The genetic distances were computed from the similarity index of Lynch and the dissimilarity measures proposed by Smouse and Peakall. The cluster analyses were based on the unweighted pair-group method using arithmetic averages and Tocher method. The clustering efficiency was assessed by the error rate of the discriminant analysis. We also performed a principal coordinates analysis. The population structure analysis revealed three tropical heterotic pools, which have been used by worldwide and Brazilian maize seed companies. The degree of genetic differentiation and of intra- and inter-population genetic diversity for these tropical heterotic pools are comparable to that observed for temperate and subtropical heterotic pools. The higher allelic frequency variation within each tropical heterotic pool and the high genetic diversity between the inbreds were evidence of heterotic groups within the main tropical heterotic pools

Avaliação de curvas de maturação de laranjas por análise de agrupamento Evaluation of different orange maturation curves by means of cluster analysis

Foram avaliadas curvas de maturação de frutos de 17 clones e cultivares de laranjas-doces pela análise de agrupamento. Determinou-se o &deg;Brix e a acidez das laranjas no período de agosto a dezembro de 1995, em Cordeirópolis, SP, obtendo-se as curvas que descrevem o comportamento das variáveis ao longo do tempo. Com base no ajustamento de equações polinomiais, foram calculadas estimativas médias para &deg;Brix e acidez aos 70, 75, 80, 85 e 90 dias após o início da coleta de frutos para análises. Com os dados padronizados, obteve-se o agrupamento pela média de grupos de pares não balanceados. Distinguiram-se quatro grupos de clones e cultivares de laranjas quanto à maturação. Um dos grupos, formado apenas pelo clone Navelência, apresentou 13,4&deg;Brix e acidez de 0,83%, no início do período considerado, mostrando maturação precoce em relação aos outros clones e cultivares. A cultivar Pêra também formou um grupo isolado, com a razão &deg;Brix/acidez superior a 12,0, em meados de outubro. Para o grupo formado pelas laranjas Natal, Folha Murcha, Old Bud Line, Cutter, Valência, Lue Ging Gong e Tuxpan foi revelada a segunda quinzena de novembro como a época adequada para colheita, enquanto que, o agrupamento dos clones Frost, Whits, Olinda, Late, Stone, Chaffei, Werley e Berry atingiu a mesma relação de sólidos solúveis e acidez após 40 dias.<br>Fruit maturation of seventeen sweet orange clones and cultivars were evaluated by cluster analysis. Soluble solids (°Brix) and acidity were determined during the spring season 1995, in Cordeirópolis, SP, Brazil. Polynomial equations were determined between August and December and values for those variables were estimated 70, 75, 80, 85 and 90 days after the initial fruit sampling. The data set was standardized to perform cluster analysis regarding the Euclidean distance measure and the linkage rule as UPGMA type. Four clusters were selected in regard to fruit maturation stage. Navelência, the earliest clone, showed 13.4°Brix and 0.83% acidity in the beginning of the considered period. The Pêra cultivar, another single clone cluster, showed ratio higher than 12.0 after middle October. The Natal, Folha Murcha, Old Bud Line, Cutter, Valência, Lue Ging Gong and Tuxpan orange clones and cultivars formed a third cluster, which reached harvesting maturity during middle November, whereas, Frost, Whits, Olinda, Late, Stone, Chaffei, Werley and Berry clones reached the same stage forty days later

Genetic control of the angular leaf spot reaction in common bean leaves and pods

Information about genetic control of plant reaction to pathogens is essential in plant breeding programs focusing resistance. This study aimed to obtain information about genetic control of the angular leaf spot reaction in leaves and pods from common bean (Phaseolus vulgaris L.) line ESAL 686. This line was crossed with cultivars Jalo EEP 558 (resistant), Cornell 49-242 (resistant) and Carioca MG (susceptible). Generations F1, F2 and backcrosses (BC11 and BC21) were obtained. In the dry season (2009), parents and respective populations were evaluated for angular leaf spot reaction under field conditions. Disease severity was evaluated on leaves and pods using diagrammatic scales. Severity scores were obtained and mean and variance genetic components were estimated for both. Segregation of F2 generation was analyzed for some crosses. Different genes control angular leaf spot reaction in leaves and pods. Mean and variance components showed predominance of additive effects. Heritability was high, however, was greater on pods than on leaves which indicated that leaf reaction is more influenced by the environment