SDS-PAGE for identification of species belonging to the Mycobacterium fortuitum complex

We performed a study to determine the usefulness of SDS-PAGE of whole cell proteins for the characterization of species of rapidly growing mycobacteria belonging to the Mycobacterium fortuitum complex. Strains included 37 M. fortuitum, 32 M. chelonae, 10 M. peregrinum, 5 M. abscessus, and 3 M. mucogenicum. Eight collection strains (including type strains of the five species) were also included in the study. All strains yielded between 44 and 58 bands in the electrophoretograms. Intraspecies similarity showed Dice coefficients higher than 95%, with only one strain of M. fortuitum having a six-band difference (Dice coefficient 87.75%). However, interspecies similarity was always below 75%, the similarity being higher between M. fortuitum and M. peregrinum (75.51%) and between M. chelonae and M. abscessus (54.9%). Visual examination of the electrophoretograms was sufficient for species characterization. SDS-PAGE of whole cell proteins is a useful technique for identification of isolates of the M. fortuitum complex, and is easy to perform without the need for complex or expensive equipment

A high efficiency 10W MMIC PA for K-b and satellite communications

This paper discusses the design steps and experimental characterization of a monolithic microwave integrated circuit (MMIC) power amplifier developed for the next generation of K-band 17.3–20.2 GHz very high throughput satellites. The technology used is a commercially available 100-nm gate length gallium nitride on silicon process. The chip was developed taking into account the demanding constraints of the spacecraft and, in particular, carefully considering the thermal constraints of such technology, in order to keep the junction temperature in all devices below 160°C in the worst-case condition (i.e., maximum environmental temperature of 85°C). The realized MMIC, based on a three-stage architecture, was first characterized on-wafer in pulsed regime and, subsequently, mounted in a test-jig and characterized under continuous wave operating conditions. In 17.3–20.2 GHz operating bandwidth, the built amplifier provides an output power >40 dBm with a power added efficiency close to 30% (peak >40%) and 22 dB of power gain

Aplicación de técnicas avanzadas de lienalización a sistemas de RF/microondas

Postprint (published version

Interaction of Hydrogen with Graphitic Surfaces, Clean and Doped with Metal Clusters

Producción CientíficaHydrogen is viewed as a possible alternative to the fossil fuels in transportation. The technology of fuel-cell engines is fully developed, and the outstanding remaining problem is the storage of hydrogen in the vehicle. Porous materials, in which hydrogen is adsorbed on the pore walls, and in particular nanoporous carbons, have been investigated as potential onboard containers. Furthermore, metallic nanoparticles embedded in porous carbons catalyze the dissociation of hydrogen in the anode of the fuel cells. For these reasons the interaction of hydrogen with the surfaces of carbon materials is a topic of high technological interest. Computational modeling and the density functional formalism (DFT) are helping in the task of discovering the basic mechanisms of the interaction of hydrogen with clean and doped carbon surfaces. Planar and curved graphene provide good models for the walls of porous carbons. We first review work on the interaction of molecular and atomic hydrogen with graphene and graphene nanoribbons, and next we address the effects due to the presence of metal clusters on the surface because of the evidence of their role in enhancing hydrogen storage.Ministerio de Economía, Industria y Competitividad (Grant MAT2014-54378-R

Fully relativistic calculation of magnetic properties of Fe, Co and Ni adclusters on Ag(100)

We present first principles calculations of the magnetic moments and magnetic anisotropy energies of small Fe, Co and Ni clusters on top of a Ag(100) surface as well as the exchange-coupling energy between two single adatoms of Fe or Co on Ag(100). The calculations are performed fully relativistically using the embedding technique within the Korringa-Kohn-Rostoker method. The magnetic anisotropy and the exchange-coupling energies are calculated by means of the force theorem. In the case of adatoms and dimers of iron and cobalt we obtain enhanced spin moments and, especially, unusually large orbital moments, while for nickel our calculations predict a complete absence of magnetism. For larger clusters, the magnitudes of the local moments of the atoms in the center of the cluster are very close to those calculated for the corresponding monolayers. Similar to the orbital moments, the contributions of the individual atoms to the magnetic anisotropy energy strongly depend on the position, hence, on the local environment of a particular atom within a given cluster. We find strong ferromagnetic coupling between two neighboring Fe or Co atoms and a rapid, oscillatory decay of the exchange-coupling energy with increasing distance between these two adatoms.Comment: 8 pages, ReVTeX + 4 figures (Encapsulated Postscript), submitted to PR

N-type graphene induced by dissociative H-2 adsorption at room temperature

Studies of the interaction between hydrogen and graphene have been increasingly required due to the indispensable modulation of the electronic structure of graphene for device applications and the possibility of using graphene as a hydrogen storage material. Here, we report on the behaviour of molecular hydrogen on graphene using the gate voltage-dependent resistance of single-, bi-, and multi-layer graphene sheets as a function of H-2 gas pressure up to 24 bar from 300 K to 345 K. Upon H-2 exposure, the charge neutrality point shifts toward the negative gate voltage region, indicating n-type doping, and distinct Raman signature changes, increases in the interlayer distance of multi-layer graphene, and a decrease in the d-spacing occur, as determined by TEM. These results demonstrate the occurrence of dissociative H-2 adsorption due to the existence of vacancy defects on graphene.open12

Desde la transferencia universitaria hacia la gestión municipal

Las profundas y aceleradas transformaciones generan modelos distintos de organización territorial que requieren, por un lado del conocimiento científico y de la valoración del ambiente, y por otro saber actuar en el contexto de las realidades locales y regionales, modificando las formas de accionar de los municipios ante necesidades específicas. El Atlas Digital del Partido de Balcarce (Tomas et al, 2004), surgió a partir de un Proyecto de Extensión del Área de Cartografía del Centro de Geología de Costas y del Cuaternario (C.G.C. y C.). Unidad de investigación, dependiente de la Facultad de Ciencias Exactas y Naturales de la Universidad Nacional de Mar del Plata, Argentina. En el año 2003 se firmó el CONTRATO DE ASESORAMIENTO Y ASISTENCIA TÉCNICA entre la Facultad de Ciencias Exactas y Naturales de la Universidad Nacional de Mar del Plata y la Municipalidad del Partido de Balcarce para la realización del Atlas Digital. El Atlas Digital del Partido de Balcarce ha sido desarrollado por un equipo de trabajo multidisciplinario, integrado por profesionales dedicados a la temática respectiva. Es una obra digital realizada con modernos recursos de captura y administración de la información espacial, obtenida de las fuentes de datos provinciales y nacionales más confiables.Fil: Tomas, Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Farenga, M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Martínez, Gustavo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exáctas y Naturales; Argentina.Fil: Massone, H. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Cabria, F. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Dillon, G. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Calandroni, M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Mazzanti, D. Universidad Nacional de Mar del Plata. Facultad de Humanidades; Argentina.Fil: Pastoriza, Elisa. Universidad Nacional de Mar del Plata. Facultad de Humanidades; Argentina.Fil: Pilcic, T. Universidad Nacional de Mar del Plata. Facultad de Humanidades; Argentina.Fil: Lanari, María Estela. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: López, María Teresa. Universidad Nacional de Mar del Plata. Facultad de Ciencias Económicas y Sociales; Argentina.Fil: López, J. Universidad Nacional de Mar del Plata. Departamento de Televisión; Argentina.Fil: Salgado, P. Universidad Nacional de Mar del Plata. Departamento de Televisión; Argentina

131I-metaiodobenzylguanidine (131I-MIBG) therapy for residual neuroblastoma: a mono-institutional experience with 43 patients

Incomplete response to therapy may compromise the outcome of children with advanced neuroblastoma. In an attempt to improve tumour response we incorporated 131I-metaiodobenzylguanidine (131I-MIBG) in the treatment regimens of selected stage 3 and stage 4 patients. Between 1986 and 1997, 43 neuroblastoma patients older than 1 year at diagnosis, 13 with stage 3 (group A) and 30 with stage 4 disease (group B) who had completed the first-line protocol without achieving complete response entered in this study. 131I-MIBG dose/course ranged from 2.5 to 5.5 Gbq (median, 3.7). The number of courses ranged from 1 to 5 (median 3) depending on the tumour response and toxicity. The most common acute side-effect was thrombocytopenia. Later side-effects included severe interstitial pneumonia in one patient, acute myeloid leukaemia in two, reduced thyroid reserve in 21. Complete response was documented in one stage 4 patient, partial response in 12 (two stage 3, 10 stage 4), mixed or no response in 25 (ten stage 3, 15 stage 4) and disease progression in five (one stage 3, four stage 4) Twenty-four patients (12/13 stage 3, 12/30 stage 4) are alive at 22–153 months (median, 59) from diagnosis. 131I-MIBG therapy may increase the cure rate of stage 3 and improve the response of stage 4 neuroblastoma patients with residual disease after first-line therapy. A larger number of patients should be treated to confirm these results but logistic problems hamper prospective and coordinated studies. Long-term toxicity can be severe. © 1999 Cancer Research Campaig