Pathogenicity and environmental tolerance of commercial and UK native entomopathogenic nematodes (Steinernema and Heterorhabditis spp.) to the larvae of mosquitoes (Aedes aegypti and Ochlerotatus detritus)

Many vector mosquito species have evolved resistance to chemical insecticides and the search for novel biological control strategies warrants further attention. Entomopathogenic nematodes (EPNs) (Heterorhabditis and Steinernema spp.) have been developed as biological control agents for use against agricultural pests but whether they could be used to control aquatic mosquito larvae warrants further research. We exposed Aedes aegypti and Ochlerotatus detritus larvae to commercially available (Steinernema feltiae, S. carpocapsae, S. kraussei and Heterorhabditis bacteriophora) and wild isolated (S. affine and S. glaseri) EPNs and monitored survival over 7 days. We also exposed EPNs to water with a range of salinities and pHs found in the marshland habitats of British mosquitoes. Ae. aegypti and O. detritus were killed by commercial EPNs, but wild strains were unable to kill Ae. aegypti yet did kill O. detritus. All EPNs were capable of tolerating a wide range of pHs but showed variable tolerance to different salinities. EPNs could be used as an alternative to chemical insecticides but target species and habitat may influence choice of EPN strain in control operations

A survey investigating the diversity and distribution of entomopathogenic nematodes in the UK and the first confirmed UK record of Steinernema carpocapsae

Entomopathogenic nematodes (EPN) of the families Steinernematidae and Heterorhabditidae are lethal insect parasites that have been commercialised as biological control agents. EPN have been isolated from across the world but it has been more than 20 years since the last survey of the UK, and species like Steinernema carpocapsae have never been found here and positively identified through molecular biology. We collected 518 soil samples from a diverse range of habitats across the UK and baited them with Galleria mellonella to isolate EPN. Dead G. mellonella were placed in White traps and emergent EPN underwent DNA barcoding analyses. From the 518 samples, 3.5% were positive for EPN. No Heterorhabditis species were found, but seven isolates of S. glaseri, one isolate of S. feltiae, eight isolates of S. affine and two isolates of S. carpocapsae were found. This was the first confirmed record of S. carpocapsae in the UK

Excitation of standing kink oscillations in coronal loops

In this work we review the efforts that have been done to study the excitation of the standing fast kink body mode in coronal loops. We mainly focus on the time-dependent problem, which is appropriate to describe flare or CME induced kink oscillations. The analytical and numerical studies in slab and cylindrical loop geometries are reviewed. We discuss the results from very simple one-dimensional models to more realistic (but still simple) loop configurations. We emphasise how the results of the initial value problem complement the eigenmode calculations. The possible damping mechanisms of the kink oscillations are also discussed

Evolution of microstructure and crystallographic texture during dissimilar friction stir welding of duplex stainless steel to low carbon-manganese structural steel

Electron backscattered diffraction (EBSD) was used to analyze the evolution of microstructure and crystallographic texture during friction stir welding of dissimilar type 2205 duplex stainless steel (DSS) to type S275 low carbon-manganese structural steel. The results of microstructural analyses show that the temperature in the center of stirred zone reached temperatures between Ac 1 and Ac 3 during welding, resulting in a minor ferrite-to-austenite phase transformation in the S275 steel, and no changes in the fractions of ferrite and austenite in the DSS. Temperatures in the thermomechanically affected and shoulder-affected zones of both materials, in particular toward the root of the weld, did not exceed the Ac 1 of S275 steel. The shear generated by the friction between the material and the rotating probe occurred in austenitic/ferritic phase field of the S275 and DSS. In the former, the transformed austenite regions of the microstructure were transformed to acicular ferrite, on cooling, while the dual-phase austenitic/ferritic structure of the latter was retained. Studying the development of crystallographic textures with regard to shear flow lines generated by the probe tool showed the dominance of simple shear components across the whole weld in both materials. The ferrite texture in S275 steel was dominated by D 1, D 2, E, E¯ , and F, where the fraction of acicular ferrite formed on cooling showed a negligible deviation from the texture for the ideal shear texture components of bcc metals. The ferrite texture in DSS was dominated by D 1, D 2, I, I¯ , and F, and that of austenite was dominated by the A, A¯ , B, and B¯ of the ideal shear texture components for bcc and fcc metals, respectively. While D 1, D 2, and F components of the ideal shear texture are common between the ferrite in S275 steel and that of dual-phase DSS, the preferential partitioning of strain into the ferrite phase of DSS led to the development of I and I¯ components in DSS, as opposed to E and E¯ in the S275 steel. The formations of fine and ultrafine equiaxed grains were observed in different regions of both materials that are believed to be due to strain-induced continuous dynamic recrystallization (CDRX) in ferrite of both DSS and S275 steel, and discontinuous dynamic recrystallization (DDRX) in austenite phase of DSS

Leaf-level productivity traits in Populus grown in short rotation coppice for biomass energy

Populus is a genus of extremely fast-growing trees originating in the northern hemisphere, with potential as a source of renewable, biomass energy. The aim of this work was to physiologically characterize genotypes of hybrid poplar, informing future selection, breeding and the development of process-based yield models. Two experiments – a short rotation coppice field trial at two contrasting UK sites and a glasshouse experiment – were conducted on five different genotypes of Populus. The field trial experiment showed that stemwood yields varied between 5.8 and 11.8 t ha?1 a?1 and that the genotype, Hoogvorst (Populus trichocarpa × P. deltoides) was the most productive. This production was characterized at a physiological level by rapid rates of leaf expansion and slow rates of leaf production and, at a cellular level, by the largest epidermal cell number per leaf. The glasshouse experiment confirmed the superior productivity of Hoogvorst, with this genotype producing the largest individual leaf areas (P 0.005) that were highly correlated with biomass production (R2 = 0.7). There was no correlation between leaf-level photosynthesis measurements and total biomass production. In general, leaf level photosynthetic characteristics were less able to differentiate between the most productive and less productive genotypes than morphological traits

Nucleation and aggregative growth of palladium nanoparticles on carbon electrodes: Experiment and kinetic model

The mechanism and kinetics of the electrochemical nucleation and growth of palladium (Pd) nanoparticles (NPs) on carbon electrodes have been investigated using a microscale meniscus cell on both highly oriented pyrolytic graphite (HOPG) and a carbon-coated transmission electron microscopy (TEM) grid. Using a microscale meniscus cell, it is possible to monitor the initial stage of electrodeposition electrochemically, while the ability to measure directly on a TEM grid allows subsequent high-resolution microscopy characterization which provides detailed nanoscopic and kinetic information. TEM analysis clearly shows that Pd is electrodeposited in the form of NPs (approximately 1–2 nm diameter) that aggregate into extensive nanocrystal-type structures. This gives rise to a high NP density. This mechanism is shown to be consistent with double potential step chronoamperometry measurements on HOPG, where a forward step generates electrodeposited Pd and the reverse step oxidizes the surface of the electrodeposited Pd to Pd oxide. The charge passed in these transients can be used to estimate the amounts of NPs electrodeposited and their size. Good agreement is found between the electrochemically determined parameters and the microscopy measurements. A model for electrodeposition based on the nucleation of NPs that aggregate to form stable structures is proposed that is used to analyze data and extract kinetics. This simple model reveals considerable information on the NP nucleation rate, the importance of aggregation in the deposition process, and quantitative values for the aggregation rate