Closely related alpha-tropomyosin mRNAs in quail fibroblasts and skeletal muscle cells.

We describe the analysis of two quail cDNA clones representing distinct but closely related alpha-tropomyosin mRNAs. cDNA clone cC101 corresponds to a 1.2-kilobase RNA which accumulates to high levels during myoblast differentiation and which encodes the major isoform of skeletal muscle alpha-tropomyosin. cDNA clone cC102 corresponds to a 2-kilobase RNA which is abundant in cultured embryonic skin fibroblasts and which encodes one of two alpha-tropomyosin-related fibroblast tropomyosins of 35,000 and 34,000 daltons apparent molecular mass (class 1 tropomyosins). The cC102 protein is unique among reported nonstriated-muscle tropomyosins in being identical in amino acid sequence to the major isoform of skeletal muscle alpha-tropomyosin over an uninterrupted stretch of at least 183 amino acids (residues 75-257). The two protein sequences differ in the COOH-terminal region beginning with residue 258. Because the cC101 and cC102 RNAs share an extensive region (at least 373 nucleotides) of nucleotide sequence identity upstream of the codon for residue 258, they are likely derived from a single gene by alternative RNA splicing, as was recently proposed in the case of related beta-tropomyosin mRNAs in human fibroblasts and skeletal muscle (MacLeod, A. R., Houlker, C., Reinach, R. C., Smillie, L. B., Talbot, K., Modi, G., and Walsh, F. S. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 7835-7837). No alpha-tropomyosin-related RNAs are abundant in undifferentiated myoblasts. This suggests the possibility of a fibroblast-specific function, as opposed to a general nonmuscle-cell function for class 1 tropomyosins and also has implications for the regulation of alpha-tropomyosin gene expression during embryonic development

Relationship between physical attributes, clay fraction mineralogy and aluminum forms in soil

O alumínio (Al) é um dos elementos mais abundantes em massa nos solos brasileiros, estando presente em diferentes minerais e formas. Considerando a carência de informações sobre a sua participação no comportamento físico do solo, buscou-se, no presente estudo, avaliar a relação entre a mineralogia da fração argila, formas de Al e atributos físicos de diferentes solos. Para tanto, foram coletadas amostras deformadas e indeformadas dos horizontes A e B de solos representativos das Zonas Fisiográficas Sul e Campos das Vertentes do estado de Minas Gerais. As amostras foram submetidas às caracterizações física, química e mineralógica. Estudos de correlação mostraram que os atributos físicos dos solos estudados estavam associados às diferentes formas de Al presentes e à mineralogia da fração argila: as formas de Al amorfas e menos cristalinas associaram-se mais aos atributos físicos relacionados com a agregação das partículas do solo, podendo esta associação ser atribuída à afinidade deste elemento com a matéria orgânica, enquanto os componentes cristalinos associaram-se mais aos atributos físicos relacionados com a organização estrutural dos solos._________________________________________________________________________________ ABSTRACT: Aluminum (Al) is one of the most abundant elements in weight in Brazilian soils, found in different minerals and forms. Considering the lack of information about its participation in the physical behavior of soil, the present study aimed to evaluate the relationship between the mineralogy of the clay fraction, different forms of Al and physical attributes of different soils. Disturbed and undisturbed samples of A and B horizons of representative soils from the South and Campos das Vertentes physiographic regions in the State of Minas Gerais were therefore collected. The samples were subjected to physical, chemical and mineralogical characterizations. Correlation studies performed with the resulting data from the different characterizations showed that the physical attributes of the different studied soils are associated to the different forms of Al and to the mineralogy of the clay fraction: the amorphous and less crystalline forms of Al were more associated to physical attributes related with particle aggregation, such association being ascribed to the affinity of this element with organic matter, while the crystalline components were more associated to physical attributes related with the structural organization of the soils

QSulf1, a heparan sulfate 6-O-endosulfatase, inhibits fibroblast growth factor signaling in mesoderm induction and angiogenesis

The signaling activities of multiple developmental ligands require sulfated heparan sulfate (HS) proteoglycans as coreceptors. QSulf1 and its mammalian orthologs are cell surface HS 6-O-endosulfatases that are expressed in embryonic mesodermal and neural progenitors and promote Wnt signal transduction. In this study, we have investigated the function of QSulf1 in fibroblast growth factor (FGF) signaling, which requires 6-O-sulfated HS for FGF receptor (FGFR) dimerization and tyrosine kinase activation. Here, we report that QSulf1 inhibits FGF2- and FGF4-induced mesoderm formation in the Xenopus embryo and FGF-dependent angiogenesis in the chicken embryo through 6-O-desulfation of cell surface HS. QSulf1 regulates FGF signaling through inhibition of HS-mediated FGFR1 activation by interfering with FGF–HS–FGFR1 ternary complex formation. Furthermore, QSulf1 can produce enzymatically modified soluble heparin that acts as a potent inhibitor of FGF2-induced angiogenesis in the chicken embryo. QSulf1, therefore, has dual regulatory functions as a negative regulator of FGF signaling and a positive regulator of Wnt signaling. Therefore, QSulf1 provides another reagent to produce enzymatically modified heparin compounds, in vivo and in vitro, to modulate cellular signaling in stem cell-based therapies to promote tissue regeneration and in cancer therapies to control cell growth and block angiogenesis

CO2 uptake potential due to concrete carbonation: A case study

The cement manufacturing process accounts for about 5% CO2 (carbon dioxide) released into the atmosphere. However, during its life cycle, concrete may capture CO2 through carbonation, in order to, partially, offset the impact of its production. Thus, this paper aims at studying the CO2 uptake potential of the Itaipu Dam due to concrete carbonation of such material. So, 155 cores were extracted from the concrete dam in different points to measure carbonation depth. In order to evaluate its influence on carbonation, the measurement of internal moisture distribution in concrete was also carried out. The results have shown that carbonation takes part of the whole dam area, indicating CO2 uptake potential. Up to the present moment, 13,384 tons of CO2 have been absorbed by concrete carbonation of the Itaipu Dam

Selective repression of myoD transcription by v-Myc prevents terminal differentiation of quail embryo myoblasts transformed by the MC29 strain of avian myelocytomatosis virus

We have investigated the mechanism by which expression of the v-myc oncogene interferes with the competence of primary quail myoblasts to undergo terminal differentia- tion. Previous studies have established that quail myoblasts transformed by myc oncogenes are severely impaired in the accumulation of mRNAs encoding the myogenic transcription factors Myf-5, MyoD and Myogenin. However, the mechanism responsible for such a repression remains largely unknown. Here we present evidence that v-Myc selectively interferes with quail myoD expression at the transcriptional level. Cis- regulatory elements involved in the auto-activation of qmyoD are speci®cally targeted in this unique example of transrepression by v-Myc, without the apparent participation of Myc-speci®c E-boxes or InR sequences. Transiently expressed v-Myc e ciently interfered with MyoD-dependent transactivation of the qmyoD regula- tory elements, while the myogenin promoter was unaffected. Finally, we show that forced expression of MyoD in v-myc-transformed quail myoblasts restored myogenin expression and promoted extensive terminal differentiation. These data suggest that transcriptional repression of qmyoD is a major and rate-limiting step in the molecular pathway by which v-Myc severely inhibits terminal differentiation in myogenic cells