Genomic analysis of 48 paenibacillus larvae bacteriophages

Indexación: Scopus.Funding: Research at UNLV was funded by National Institute of General Medical Sciences grant GM103440 (NV INBRE), the UNLV School of Life Sciences, and the UNLV College of Sciences. E.C.-N. was funded by CONICYT-FONDECYT de iniciación en la investigación 11160905. Research at BYU was funded by the BYU Microbiology & Molecular Biology Department, and private donations through LDS Philanthropies.The antibiotic-resistant bacterium Paenibacillus larvae is the causative agent of American foulbrood (AFB), currently the most destructive bacterial disease in honeybees. Phages that infect P. larvae were isolated as early as the 1950s, but it is only in recent years that P. larvae phage genomes have been sequenced and annotated. In this study we analyze the genomes of all 48 currently sequenced P. larvae phage genomes and classify them into four clusters and a singleton. The majority of P. larvae phage genomes are in the 38–45 kbp range and use the cohesive ends (cos) DNA-packaging strategy, while a minority have genomes in the 50–55 kbp range that use the direct terminal repeat (DTR) DNA-packaging strategy. The DTR phages form a distinct cluster, while the cos phages form three clusters and a singleton. Putative functions were identified for about half of all phage proteins. Structural and assembly proteins are located at the front of the genome and tend to be conserved within clusters, whereas regulatory and replication proteins are located in the middle and rear of the genome and are not conserved, even within clusters. All P. larvae phage genomes contain a conserved N-acetylmuramoyl-L-alanine amidase that serves as an endolysin. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.https://www.mdpi.com/1999-4915/10/7/37

Comparative Genomics Analysis of a New Exiguobacterium Strain from Salar de Huasco Reveals a Repertoire of Stress-Related Genes and Arsenic Resistance

Indexación: Web of Science; Scopus.The Atacama Desert hosts diverse ecosystems including salt flats and shallow Andean lakes. Several heavy metals are found in the Atacama Desert, and microorganisms growing in this environment show varying levels of resistance/tolerance to copper, tellurium, and arsenic, among others. Herein, we report the genome sequence and comparative genomic analysis of a new Exiguobacterium strain, sp. SH31, isolated from an altiplanic shallow athalassohaline lake. Exiguobacterium sp. SH31 belongs to the phylogenetic Group II and its closest relative is Exiguobacterium sp. S17, isolated from the Argentinian Altiplano (95% average nucleotide identity). Strain SH31 encodes a wide repertoire of proteins required for cadmium, copper, mercury, tellurium, chromium, and arsenic resistance. Of the 34 Exiguobacterium genomes that were inspected, only isolates SH31 and S17 encode the arsenic efflux pump Acr3. Strain SH31 was able to grow in up to 10 mM arsenite and 100 mM arsenate, indicating that it is arsenic resistant. Further, expression of the ars operon and acr3 was strongly induced in response to both toxics, suggesting that the arsenic efflux pump Acr3 mediates arsenic resistance in Exiguobacterium sp. SH31.http://journal.frontiersin.org/article/10.3389/fmicb.2017.00456/ful

An approach to phenotypic analysis and environmental variability. The examples of the genera Dianthus L. and Lotus L. in the north of Portugal

The analysis of the correlation between phenotypic plasticity and environmental variability has been very useful to describe the morphologic responses of individuals to environmental factors affecting them (in the present contribution the altitudinal variation), from a genecological perpective. However, the literature shows that the studies in this area have had a monospecific or monogeneric use. As a consequence, intergeneric comparative descriptions have not been possible. In the present work we propose an analytical method to compare the morphological expressivity of individuals included in the genera Dianthus and Lotus from the north of Portugal by means of a contingency matrix. This matrix was elaborated on the basis of amplitude parameters, in order to describe the variability present in the basic matrix of each genus studied. The results pointed out the existence of opposite phenotypic behaviours dependent on the altitudinal variation, thus indicating the importance of the analysis between different groups of taxa as a reference for the comparison of their morphological expressivities

Apoio Matricial Como Articulador Das Relações Interprofissionais Entre Serviços Especializados E Atenção Primária à Saúde

This paper sought to carry out an integrative review of the national production on Matrix Support methodology, whilst encompassing empirical studies on effectiveness and theoretical-methodological review papers on Matrix Support. The used material is comprised of papers that are indexed in the PubMed database, the NLM and the BVS (Virtual Library of Health), between 1999 and 2013, in Portuguese, English and Spanish. We included 28 articles, and its analysis allowed the identification of three categories: 1) foundation goods and consolidation of the concept of matrix support; 2) Review articles on the matrix support; 3) Items arising from empirical research involving the concept of matrix support. The publications showed that the matrix support brings an innovative proposal and powerful to transform the health care model. However, to achieve their goals is necessary to overcome some impasses, several interconnected directly with the implementation of their directives and to overcoming structural problems of the National Health System (SUS). © 2016, Institute de Medicina Social da UERJ. All rights reserved.26245548

Apoio Matricial No Sus Campinas: Análise Da Consolidação De Uma Prática Interprofissional Na Rede De Saúde

This study aims to characterize the teams and the inter-professional work process of Matrix Support developed and practiced in primary healthcare provided by the Brazilian Unified Health System (SUS) in Campinas, São Paulo State, Brazil. This is an exploratory descriptive study involving a questionnaire that was applied to 232 professionals who practice Matrix Support for primary healthcare. For analysis, the data were grouped into four categories: Identification of the professional; Work links to the Campinas SUS; Organization of the Matrix Support work; and the Support practice. The study indicates that the methodology of support for inter-professional work has achieved an important degree of consolidation in the municipality, in spite of the restricted investment. The reduced working time dedicated to support, and the large number of teams supported by each Matrix Support team were identified as the principal points of fragility in the work process. In turn, strong points that emerged were the multiplicity of tools used, the possibility of shared construction of work guidelines, and the flexibility in the composition of the support teams. Both the fragilities and the potentialities found can offer inputs for reflection and full creation of Matrix Support in other contexts. © 2016, Associacao Brasileira de Pos - Graduacao em Saude Coletiva. All rights reserved.2151625163

Bosonization in Higher Dimensions

Using the recently discovered connection between bosonization and duality transformations (hep-th/9401105 and hep-th/9403173), we give an explicit path-integral representation for the bosonization of a massive fermion coupled to a U(1) gauge potential (such as electromagnetism) in d space (D=d+1 spacetime) dimensions. The bosonic theory is described by a rank d-1 antisymmetric Kalb-Ramond-type gauge potential. We construct the bosonized lagrangian explicitly in the limit of large fermion mass. We find that the resulting action is local for d=2 (and given by a Chern-Simons action), but nonlocal for d larger than 3. By coupling to a statistical Chern-Simons field for d=2, we obtain a bosonized formulation of anyons. The bosonic theory may be further dualized to a theory involving purely scalars, for any d, and we show this to be governed by a higher-derivative lagrangian for which the scalar decouples from the U(1) gauge potential.Comment: (We had omitted some references and had misspelled `aficionados') plain TeX, 11 pages, McGill-94/33, NEIP-94-006, OSLO-TP 10-9

Noncommutative Electromagnetism As A Large N Gauge Theory

We map noncommutative (NC) U(1) gauge theory on R^d_C X R^{2n}_{NC} to U(N -> \infty) Yang-Mills theory on R^d_C, where R^d_C is a d-dimensional commutative spacetime while R^{2n}_{NC} is a 2n-dimensional NC space. The resulting U(N) Yang-Mills theory on R^d_C is equivalent to that obtained by the dimensional reduction of (d+2n)-dimensional U(N) Yang-Mills theory onto R^d_C. We show that the gauge-Higgs system (A_\mu,\Phi^a) in the U(N -> \infty) Yang-Mills theory on R^d_C leads to an emergent geometry in the (d+2n)-dimensional spacetime whose metric was determined by Ward a long time ago. In particular, the 10-dimensional gravity for d=4 and n=3 corresponds to the emergent geometry arising from the 4-dimensional N=4 vector multiplet in the AdS/CFT duality. We further elucidate the emergent gravity by showing that the gauge-Higgs system (A_\mu,\Phi^a) in half-BPS configurations describes self-dual Einstein gravity.Comment: 25 pages; More clarifications, to appear in Eur. Phys. J.

Genomic Variation and Arsenic Tolerance Emerged as Niche Specific Adaptations by Different Exiguobacterium Strains Isolated From the Extreme Salar de Huasco Environment in Chilean – Altiplano

Indexación: Scopus.Polyextremophilic bacteria can thrive in environments with multiple stressors such as the Salar de Huasco (SH). Microbial communities in SH are exposed to low atmospheric pressure, high UV radiation, wide temperature ranges, salinity gradient and the presence of toxic compounds such as arsenic (As). In this work we focus on arsenic stress as one of the main adverse factors in SH and bacteria that belong to the Exiguobacterium genus due to their plasticity and ubiquity. Therefore, our aim was to shed light on the effect of niche conditions pressure (particularly arsenic), on the adaptation and divergence (at genotypic and phenotypic levels) of Exiguobacterium strains from five different SH sites. Also, to capture greater diversity in this genus, we use as outgroup five As(III) sensitive strains isolated from Easter Island (Chile) and The Great Salt Lake (United States). For this, samples were obtained from five different SH sites under an arsenic gradient (9 to 321 mg/kg: sediment) and isolated and sequenced the genomes of 14 Exiguobacterium strains, which had different arsenic tolerance levels. Then, we used comparative genomic analysis to assess the genomic divergence of these strains and their association with phenotypic differences such as arsenic tolerance levels and the ability to resist poly-stress. Phylogenetic analysis showed that SH strains share a common ancestor. Consequently, populations were separated and structured in different SH microenvironments, giving rise to multiple coexisting lineages. Hence, this genotypic variability is also evidenced by the COG (Clusters of Orthologous Groups) composition and the size of their accessory genomes. Interestingly, these observations correlate with physiological traits such as growth patterns, gene expression, and enzyme activity related to arsenic response and/or tolerance. Therefore, Exiguobacterium strains from SH are adapted to physiologically overcome the contrasting environmental conditions, like the arsenic present in their habitat. © Copyright © 2020 Castro-Severyn, Pardo-Esté, Mendez, Morales, Marquez, Molina, Remonsellez, Castro-Nallar and Saavedra.https://www.frontiersin.org/articles/10.3389/fmicb.2020.01632/ful

Condensed matter and AdS/CFT

I review two classes of strong coupling problems in condensed matter physics, and describe insights gained by application of the AdS/CFT correspondence. The first class concerns non-zero temperature dynamics and transport in the vicinity of quantum critical points described by relativistic field theories. I describe how relativistic structures arise in models of physical interest, present results for their quantum critical crossover functions and magneto-thermoelectric hydrodynamics. The second class concerns symmetry breaking transitions of two-dimensional systems in the presence of gapless electronic excitations at isolated points or along lines (i.e. Fermi surfaces) in the Brillouin zone. I describe the scaling structure of a recent theory of the Ising-nematic transition in metals, and discuss its possible connection to theories of Fermi surfaces obtained from simple AdS duals.Comment: 39 pages, 12 figures; Lectures at the 5th Aegean summer school, "From gravity to thermal gauge theories: the AdS/CFT correspondence", and the De Sitter Lecture Series in Theoretical Physics 2009, University of Groninge