The bacterial biome of ticks and their wildlife hosts at the urban–wildland interface

Advances in sequencing technologies have revealed the complex and diverse microbial communities present in ticks (Ixodida). As obligate blood-feeding arthropods, ticks are responsible for a number of infectious diseases that can affect humans, livestock, domestic animals and wildlife. While cases of human tick-borne diseases continue to increase in the northern hemisphere, there has been relatively little recognition of zoonotic tick-borne pathogens in Australia. Over the past 5 years, studies using high-throughput sequencing technologies have shown that Australian ticks harbour unique and diverse bacterial communities. In the present study, free-ranging wildlife (n=203), representing ten mammal species, were sampled from urban and peri-urban areas in New South Wales (NSW), Queensland (QLD) and Western Australia (WA). Bacterial metabarcoding targeting the 16S rRNA locus was used to characterize the microbiomes of three sample types collected from wildlife: blood, ticks and tissue samples. Further sequence information was obtained for selected taxa of interest. Six tick species were identified from wildlife: Amblyomma triguttatum, Ixodes antechini, Ixodes australiensis, Ixodes holocyclus, Ixodes tasmani and Ixodes trichosuri. Bacterial 16S rRNA metabarcoding was performed on 536 samples and 65 controls, generating over 100 million sequences. Alpha diversity was significantly different between the three sample types, with tissue samples displaying the highest alpha diversity (P<0.001). Proteobacteria was the most abundant taxon identified across all sample types (37.3 %). Beta diversity analysis and ordination revealed little overlap between the three sample types (P<0.001). Taxa of interest included Anaplasmataceae, Bartonella, Borrelia, Coxiellaceae, Francisella, Midichloria, Mycoplasma and Rickettsia. Anaplasmataceae bacteria were detected in 17.7% (95/536) of samples and included Anaplasma, Ehrlichia and Neoehrlichia species. In samples from NSW, ‘Ca. Neoehrlichia australis’, ‘Ca. Neoehrlichia arcana’, Neoehrlichia sp. and Ehrlichia sp. were identified. A putative novel Ehrlichia sp. was identified from WA and Anaplasma platys was identified from QLD. Nine rodent tissue samples were positive for a novel Borrelia sp. that formed a phylogenetically distinct clade separate from the Lyme Borrelia and relapsing fever groups. This novel clade included recently identified rodent-associated Borrelia genotypes, which were described from Spain and North America. Bartonella was identified in 12.9% (69/536) of samples. Over half of these positive samples were obtained from black rats (Rattus rattus), and the dominant bacterial species identified were Bartonella coopersplainsensis and Bartonella queenslandensis. The results from the present study show the value of using unbiased high-throughput sequencing applied to samples collected from wildlife. In addition to understanding the sylvatic cycle of known vector-associated pathogens, surveillance work is important to ensure preparedness for potential zoonotic spillover events

Childhood IQ and cardiovascular disease in adulthood: prospective observational study linking the Scottish Mental Survey 1932 and the Midspan studies

This study investigated the influence of childhood IQ on the relationships between risk factors and cardiovascular disease (CVD), coronary heart disease (CHD) and stroke in adulthood. Participants were from the Midspan prospective cohort studies which were conducted on adults in Scotland in the 1970s. Data on risk factors were collected from a questionnaire and at a screening examination, and participants were followed up for 25 years for hospital admissions and mortality. 938 Midspan participants were successfully matched with their age 11 IQ from the Scottish Mental Survey 1932, in which 1921-born children attending schools in Scotland took a cognitive ability test. Childhood IQ was negatively correlated with diastolic and systolic blood pressure, and positively correlated with height and respiratory function in adulthood. For each of CVD, CHD and stroke, defined as either a hospital admission or death, there was an increased relative rate per standard deviation decrease (15 points) in childhood IQ of 1.11 (95% confidence interval 1.01-1.23), 1.16 (1.03-1.32) and 1.10 (0.88-1.36) respectively. With events divided into those first occurring before and those first occurring after the age of 65, the relationships between childhood IQ and CVD, CHD and stroke were only seen before age 65 and not after age 65. Blood pressure, height, respiratory function and smoking were associated with CVD events. Relationships were stronger in the early compared to the later period for smoking and FEV1, and stronger in the later compared to the earlier period for blood pressure. Adjustment for childhood IQ had small attenuating effects on the risk factor-CVD relationship before age 65 and no effects after age 65. Adjustment for risk factors attenuated the childhood IQ-CVD relationship by a small amount before age 65. Childhood IQ was associated with CVD risk factors and events and can be considered an important new risk factor

Correlated Hybrid Fluctuations from Inflation with Thermal Dissipation

We investigate the primordial scalar perturbations in the thermal dissipative inflation where the radiation component (thermal bath) persists and the density fluctuations are thermally originated. The perturbation generated in this model is hybrid, i.e. it consists of both adiabatic and isocurvature components. We calculate the fractional power ratio ($S$) and the correlation coefficient ($\cos\Delta$) between the adiabatic and the isocurvature perturbations at the commencing of the radiation regime. Since the adiabatic/isocurvature decomposition of hybrid perturbations generally is gauge-dependent at super-horizon scales when there is substantial energy exchange between the inflaton and the thermal bath, we carefully perform a proper decomposition of the perturbations. We find that the adiabatic and the isocurvature perturbations are correlated, even though the fluctuations of the radiation component is considered uncorrelated with that of the inflaton. We also show that both $S$ and $\cos \Delta$ depend mainly on the ratio between the dissipation coefficient $\Gamma$ and the Hubble parameter $H$ during inflation. The correlation is positive ($\cos\Delta > 0$) for strong dissipation cases where $\Gamma/H >0.2$, and is negative for weak dissipation instances where $\Gamma/H <0.2$. Moreover, $S$ and $\cos \Delta$ in this model are not independent of each other. The predicted relation between $S$ and $\cos\Delta$ is consistent with the WMAP observation. Other testable predictions are also discussed.Comment: 18 pages using revtex4, accepted for publication in PR

The Scottish Mental Survey 1932 linked to the Midspan studies: a prospective investigation of childhood intelligence and future health

The Scottish Mental Survey of 1932 (SMS1932) recorded mental ability test scores for nearly all of the age group of children born in 1921 and at school in Scotland on 1st June 1932. The Collaborative and Renfrew/Paisley studies, two of the Midspan studies, obtained health and social data by questionnaire and a physical examination in the 1970s. Some Midspan participants were born in 1921 and may have taken part in the SMS1932, so might have mental ability data available from childhood. The 1921-born Midspan participants were matched with the computerised SMS1932 database. The total numbers successfully matched were 1032 out of 1251 people (82.5%). Of those matched, 938 (90.9%) had a mental ability test score recorded. The mean score of the matched sample was 37.2 (standard deviation [SD] 13.9) out of a possible score of 76. The mean (SD) for the boys and girls respectively was 38.3 (14.2) and 35.7 (13.9). This compared with 38.6 (15.7) and 37.2 (14.3) for boys and girls in all of Scotland. Graded relationships were found between mental ability in childhood, and social class and deprivation category of residence in adulthood. Being in a higher social class or in a more affluent deprivation category was associated with higher childhood mental ability scores and the scores reduced with increasing deprivation. Future plans for the matched data include examining associations between childhood mental ability and other childhood and adult risk factors for disease in adulthood, and modelling childhood mental ability, alongside other factors available in the Midspan database, as a risk factor for specific illnesses, admission to hospital and mortality

A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria

Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer

Personalised service? Changing the role of the government librarian

Investigates the feasibility of personalised information service in a government department. A qualitative methodology explored stakeholder opinions on the remit, marketing, resourcing and measurement of the service. A questionnaire and interviews gathered experiences of personalised provision across the government sector. Potential users were similarly surveyed to discuss how the service could meet their needs. Data were analysed using coding techniques to identify emerging theory. Lessons learned from government librarians centred on clarifying requirements, balancing workloads and selective marketing. The user survey showed low usage and awareness of existing specialist services, but high levels of need and interest in services repackaged as a tailored offering. Fieldwork confirmed findings from the literature on the scope for adding value through information management advice, information skills training and substantive research assistance and the need to understand business processes and develop effective partnerships. Concluding recommendations focus on service definition, strategic marketing, resource utilisation and performance measurement

Modeling the magnetic field in the galactic disk using new rotation measure observations from the Very Large Array

We have determined 194 Faraday rotation measures (RMs) of polarized extragalactic radio sources using new, multi-channel polarization observations at frequencies around 1.4 GHz from the Very Large Array (VLA) in the Galactic plane at 17◦ ≤ l ≤ 63◦ and 205◦ ≤ l ≤ 253◦. This catalog fills in gaps in the RM coverage of the Galactic plane between the Canadian Galactic Plane Survey and Southern Galactic Plane Survey. Using this catalog we have tested the validity of recently-proposed axisymmetric and bisymmetric models of the largescale (or regular) Galactic magnetic field, and found that of the existing models we tested, an axisymmetric spiral model with reversals occurring in rings (as opposed to along spiral arms) best matched our observations. Building on this, we have performed our own modeling, using RMs from both extragalactic sources and pulsars. By developing independent models for the magnetic field in the outer and inner Galaxy, we conclude that in the inner Galaxy, the magnetic field closely follows the spiral arms, while in the outer Galaxy, the field is consistent with being purely azimuthal. Furthermore, the models contain no reversals in the outer Galaxy, and together seem to suggest the existence of a single reversed region that spirals out from the Galactic cente

Tolerance of chronic hypercapnia by the European eel Anguilla anguilla

European eels were exposed for 6 weeks to water CO2 partial pressures (PCO\ub7) from ambient (approx.0.8\ub7mmHg), through 15\ub11\ub7mmHg and 30\ub11\ub7mmHg to 45\ub11\ub7mmHg in water with a total hardness of240\ub7mg\ub7l\u20131 as CaCO3, pH 8.2, at 23\ub11\ub0C. Arterial plasma PCO\ub7 equilibrated at approximately 2\ub7mmHg above water PCO\ub7 in all groups, and plasma bicarbonate accumulated up to 72\ub7mmol\ub7l\u20131 in the group at a water PCO\ub7 of 45\ub7mmHg. This was associated with an equimolar loss of plasma Cl\u2013, which declined to 71\ub7mmol\ub7l\u20131 at the highest water PCO\ub7. Despite this, extracellular acid\u2013base compensation was incomplete; all hypercapnic groups tolerated chronic extracellular acidoses and reductions in arterial blood O2 content (CaO\ub7), of progressive severity with increasing PCO\ub7. All hypercapnic eels, however, regulated the intracellular pH of heart and white muscle to the same levels as normocapnic animals. Hypercapnia had no effect on such indicators of stress as plasma catecholamine or cortisol levels, plasma osmolality or standard metabolic rate. Furthermore, although CaO\ub7 was reduced by approximately 50% at the highest PCO\ub7, there was no effect of hypercapnia on the eels\u2019 tolerance of hypoxia, aerobic metabolic scope or sustained swimming performance. The results indicate that, at the levels tested, chronic hypercapnia was not a physiological stress for the eel, which can tolerate extracellular acidosis and extremely low Cl\u2013 levels while compensating tissue intracellular pH, and which can meet the O2 requirements of routine and active metabolism despite profound hypoxaemia

Density functional study of Aun_n (n=2-20) clusters: lowest-energy structures and electronic properties

We have investigated the lowest-energy structures and electronic properties of the Au$_n$(n=2-20) clusters based on density functional theory (DFT) with local density approximation. The small Au$_n$ clusters adopt planar structures up to n=6. Tabular cage structures are preferred in the range of n=10-14 and a structural transition from tabular cage-like structure to compact near-spherical structure is found around n=15. The most stable configurations obtained for Au$_{13}$ and Au$_{19}$ clusters are amorphous instead of icosahedral or fcc-like, while the electronic density of states sensitively depend on the cluster geometry. Dramatic odd-even alternative behaviors are obtained in the relative stability, HOMO-LUMO gaps and ionization potentials of gold clusters. The size evolution of electronic properties is discussed and the theoretical ionization potentials of Au$_n$ clusters compare well with experiments.Comment: 6 pages, 7 figure