1,141 research outputs found
Duplex-PCR for Identification and Differentiation of Cattle and Buffalo Processed Meat
A simple and reliable duplex polymerase chain reaction (duplex-PCR) technique is proposed to identify and differentiate cattle and water buffalo DNA using primers that were tested on mitochondrial DNA (mtDNA) extracted from meat muscle samples. Different levels of autolysis were experimentally produced by putrefaction and heating the samples at various temperatures and conditions to simulate the various meat processing technology. The optimized PCR amplified 113 bp and 152bp fragment of cyt b gene from mtDNA. This test was successful in detecting up to 1 pg adulteration in cattle-buffalo meat mixture. The test is a valuable tool for meat authentication and screening of cooked, putrefied and mixed samples of cattle and buffalo
The (2+1)-dimensional Gross-Neveu model with a U(1) chiral symmetry at non-zero temperature
We present results from numerical simulations of the (2+1)-dimensional
Gross-Neveu model with a U(1) chiral symmetry and N_f=4 fermion species at
non-zero temperature. We provide evidence that there are two different chirally
symmetric phases, one critical and one with finite correlation length,
separated by a Berezinskii-Kosterlitz-Thouless transition. We have also
identified a regime above the critical temperature in which the fermions
acquire a screening mass even in the absence of chiral symmetry breaking,
analogous to the pseudogap behaviour observed in cuprate superconductors.Comment: 12 pages, 6 figure
Excited Baryons in the Large Limit
The spectrum of excited -type heavy baryons is considered in the
large limit. The universal form factors for semileptonic
decay to excited charmed baryons are calculated in the large limit. We
find that the Bjorken sum rule (for the slope of the Isgur--Wise function) and
Voloshin sum rule (for the mass of the light degrees of freedom) are saturated
by the first doublet of excited states.Comment: 9 pages, use phyzzx, CALT-68-191
Differential Equations for Definition and Evaluation of Feynman Integrals
It is shown that every Feynman integral can be interpreted as Green function
of some linear differential operator with constant coefficients. This
definition is equivalent to usual one but needs no regularization and
application of -operation. It is argued that presented formalism is
convenient for practical calculations of Feynman integrals.Comment: pages, LaTEX, MSU-PHYS-HEP-Lu2/9
Rampant Centrosome Amplification Underlies more Aggressive Disease Course of Triple Negative Breast Cancers
Centrosome amplification (CA), a cell-biological trait, characterizes pre-neoplastic and pre-invasive lesions and is associated with tumor aggressiveness. Recent studies suggest that CA leads to malignant transformation and promotes invasion in mammary epithelial cells. Triple negative breast cancer (TNBC), a histologically-aggressive subtype shows high recurrence, metastases, and mortality rates. Since TNBC and non- TNBC follow variable kinetics of metastatic progression, they constitute a novel test bed to explore if severity and nature of CA can distinguish them apart. We quantitatively assessed structural and numerical centrosomal aberrations for each patient sample in a large-cohort of grade-matched TNBC (n = 30) and non-TNBC (n = 98) cases employing multi-color confocal imaging. Our data establish differences in incidence and severity of CA between TNBC and non-TNBC cell lines and clinical specimens. We found strong correlation between CA and aggressiveness markers associated with metastasis in 20 pairs of grade-matched TNBC and non-TNBC specimens (p \u3c 0.02). Time-lapse imaging of MDA-MB-231 cells harboring amplified centrosomes demonstrated enhanced migratory ability. Our study bridges a vital knowledge gap by pinpointing that CA underlies breast cancer aggressiveness. This previously unrecognized organellar inequality at the centrosome level may allow early-risk prediction and explain higher tumor aggressiveness and mortality rates in TNBC patients
Anomalous Commutator Algebra for Conformal Quantum Mechanics
The structure of the commutator algebra for conformal quantum mechanics is
considered. Specifically, it is shown that the emergence of a dimensional scale
by renormalization implies the existence of an anomaly or quantum-mechanical
symmetry breaking, which is explicitly displayed at the level of the generators
of the SO(2,1) conformal group. Correspondingly, the associated breakdown of
the conservation of the dilation and special conformal charges is derived.Comment: 23 pages. A few typos corrected in the final version (which agrees
with the published Phys. Rev. D article
HSET Overexpression Fuels Tumor Progression via Centrosome Clustering-Independent Mechanisms in Breast Cancer Patients
Human breast tumors harbor supernumerary centrosomes in almost 80% of tumor cells. Although amplified centrosomes compromise cell viability via multipolar spindles resulting in death-inducing aneuploidy, cancer cells tend to cluster extra centrosomes during mitosis. As a result cancer cells display bipolar spindle phenotypes to maintain a tolerable level of aneuploidy, an edge to their survival. HSET/KifC1, a kinesin-like minus-end directed microtubule motor has recently found fame as a crucial centrosome clustering molecule. Here we show that HSET promotes tumor progression via mechanisms independent of centrosome clustering. We found that HSET is overexpressed in breast carcinomas wherein nuclear HSET accumulation correlated with histological grade and predicted poor progression-free and overall survival. In addition, deregulated HSET protein expression was associated with gene amplification and/or translocation. Our data provide compelling evidence that HSET overexpression is pro-proliferative, promotes clonogenic-survival and enhances cellcycle kinetics through G2 and M-phases. Importantly, HSET co-immunoprecipitates with survivin, and its overexpression protects survivin from proteasome-mediated degradation, resulting in its increased steady-state levels. We provide the first evidence of centrosome clustering-independent activities of HSET that fuel tumor progression and firmly establish that HSET can serve both as a potential prognostic biomarker and as a valuable cancer-selective therapeutic target
Processing of ultrafine-size particulate metal matrix composites by advanced shear technology
Copyright @ 2009 ASM International. This paper was published in Metallurgical & Materials Transactions A 40A(3) and is made available as an electronic reprint with the permission of ASM International. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplications of any material in this paper for a fee or for commercial purposes, or modification of the content of this paper are prohibited.Lack of efficient mixing technology to achieve a uniform distribution of fine-size reinforcement within the matrix and the high cost of producing components have hindered the widespread adaptation of particulate metal matrix composites (PMMCs) for engineering applications. A new rheo-processing method, the melt-conditioning high-pressure die-cast (MC-HPDC) process, has been developed for manufacturing near-net-shape components of high integrity. The MC-HPDC process adapts the well-established high shear dispersive mixing action of a twin-screw mechanism to the task of overcoming the cohesive force of the agglomerates under a high shear rate and high intensity of turbulence. This is followed by direct shaping of the slurry into near-net-shape components using an existing cold-chamber die-casting process. The results indicate that the MC-HPDC samples have a uniform distribution of ultrafine-sized SiC particles throughout the entire sample in the as-cast condition. Compared to those produced by conventional high-pressure die casting (HPDC), MC-HPDC samples have a much improved tensile strength and ductility.EP-SR
Searching for a Cosmological Preferred Axis: Union2 Data Analysis and Comparison with Other Probes
We review, compare and extend recent studies searching for evidence for a
preferred cosmological axis. We start from the Union2 SnIa dataset and use the
hemisphere comparison method to search for a preferred axis in the data. We
find that the hemisphere of maximum accelerating expansion rate is in the
direction (\omm=0.19) while the hemisphere of
minimum acceleration is in the opposite direction
(\omm=0.30). The level of anisotropy is described by the normalized
difference of the best fit values of \omm between the two hemispheres in the
context of \lcdm fits. We find a maximum anisotropy level in the Union2 data of
\frac{\Delta \ommax}{\bomm}=0.43\pm 0.06. Such a level does not necessarily
correspond to statistically significant anisotropy because it is reproduced by
about of simulated isotropic data mimicking the best fit Union2 dataset.
However, when combined with the axes directions of other cosmological
observations (bulk velocity flow axis, three axes of CMB low multipole moments
and quasar optical polarization alignment axis), the statistical evidence for a
cosmological anisotropy increases dramatically. We estimate the probability
that the above independent six axes directions would be so close in the sky to
be less than . Thus either the relative coincidence of these six axes is a
very large statistical fluctuation or there is an underlying physical or
systematic reason that leads to their correlation.Comment: 10 pages, 7 figures. Accepted in JCAP (to appear). Extended analysis
with redshift tomography of SnIa, included errorbars and increased number of
axes. The Mathematica 7 files with the data used for the production of the
figures along with a Powerpoint file with additional figures may be
downloaded from http://leandros.physics.uoi.gr/anisotrop
Renormalized Path Integral for the Two-Dimensional Delta-Function Interaction
A path-integral approach for delta-function potentials is presented.
Particular attention is paid to the two-dimensional case, which illustrates the
realization of a quantum anomaly for a scale invariant problem in quantum
mechanics. Our treatment is based on an infinite summation of perturbation
theory that captures the nonperturbative nature of the delta-function bound
state. The well-known singular character of the two-dimensional delta-function
potential is dealt with by considering the renormalized path integral resulting
from a variety of schemes: dimensional, momentum-cutoff, and real-space
regularization. Moreover, compatibility of the bound-state and scattering
sectors is shown.Comment: 26 pages. The paper was significantly expanded and numerous equations
were added for the sake of clarity; the main results and conclusions are
unchange
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