Characterization of soil heavy metal pools in paddy fields in Taiwan: chemical extraction and solid-solution partitioning

Ongoing industrialization has resulted in an accumulation of metals like Cd, Cu, Cr, Ni, Zn, and Pb in paddy fields across Southeast Asia. Risks of metals in soils depend on soil properties and the availability of metals in soil. At present, however, limited information is available on how to measure or predict the directly available fraction of metals in paddy soils. Here, the distribution of Cd, Cu, Cr, Ni, Zn, and Pb in 19 paddy fields among the total, reactive, and directly available pools was measured using recently developed concepts for aerated soils. Solid-solution partitioning models have been derived to predict the directly available metal pool. Such models are proven to be useful for risk assessment and to derive soil quality standards for aerated soils. Soil samples (0-25 cm) were taken from 19 paddy fields from five different communities in Taiwan in 2005 and 2006. Each field was subdivided into 60 to 108 plots resulting in a database of approximately 3,200 individual soil samples. Total (Aqua Regia (AR)), reactive (0.43 M HNO3, 0.1 M HCl, and 0.05 M EDTA), and directly available metal pools (0.01 M CaCl2) were determined. Solid-solution partitioning models were derived by multiple linear regressions using an extended Freundlich equation using the reactive metal pool, pH, and the cation exchange capacity (CEC). The influence of Zn on metal partitioning and differences between both sampling events (May/November) were evaluated. Total metals contents range from background levels to levels in excess of current soil quality standards for arable land. Between 3% (Cr) and 30% (Cd) of all samples exceed present soil quality standards based on extraction with AR. Total metal levels decreased with an increasing distance from the irrigation water inlet. The reactive metal pool relative to the total metal content is increased in the order C

Assessment of spatial and temporal patterns of green and blue water flows under natural conditions in inland river basins in Northwest China

In arid and semi-arid regions freshwater resources have become scarcer with increasing demands from socioeconomic development and population growth. Until recently, water research and management has maily focused on blue water but ignored green water. Furthermore, in data poor regions hydrological low under natural conditions are poorly characterised but are a prerequisite to inform future water resources management. Here we report on spatial and temporal patterns of both blue and green water flows that can be expected under natural conditions as simulated by the Soil and Water Assessment Tool (SWAT) for the Heihe river basin, the second largest inland river basin in Northwest China. Calibration ad validation at two hydrological stations show good performance of the SWAT model in modelling hydrological processes. The total green and blue water flows were 22.05-25.51 billion m3 in the 2000s for the Heihe river basin. Blue water flows are larger in upstream sub-basins than in downstream sub-basins mainly due to high precipitation and a large amount of snow and melting water in upstream. Green water flows are distributed more homogeneously among different sub-basins. The green water coefficient was 87%-89% in the 2000s for the entire river basin, varying from around 80%90% in up-and mid-stream sub-basins to above 90% in downstream sub-basins. This is much higher than reported green water coefficients in many other river basins. The spatial patterns of green water coefficients were closely linked to dominant land covers (e.g. snow cover upstream and desert downstream) and climate conditions (e.g. high precipitation upstream and low precipitation downstream). There are no clear consistent historical trends of change in green and blue water flows and the green water coefficient at both the river basin and sub-basin levels. This study provides insights into green and blue water endowments under natural conditions for the entire Heihe river basin at the sub-basin level. The results are helpful to benchmark the natural flows of water in the basin as part of improved water resources management in the inland river basins of China

A Gaussian process and image registration based stitching method for high dynamic range measurement of precision surfaces

Optical instruments are widely used for precision surface measurement. However, the dynamic range of optical instruments, in terms of measurement area and resolution, is limited by the characteristics of the imaging and the detection systems. If a large area with a high resolution is required, multiple measurements need to be conducted and the resulting datasets needs to be stitched together. Traditional stitching methods use six degrees of freedom for the registration of the overlapped regions, which can result in high computational complexity. Moreover, measurement error increases with increasing measurement data. In this paper, a stitching method, based on a Gaussian process, image registration and edge intensity data fusion, is presented. Firstly, the stitched datasets are modelled by using a Gaussian process so as to determine the mean of each stitched tile. Secondly, the datasets are projected to a base plane. In this way, the three-dimensional datasets are transformed to two-dimensional (2D) images. The images are registered by using an (x, y) translation to simplify the complexity. By using a high precision linear stage that is integral to the measurement instrument, the rotational error becomes insignificant and the cumulative rotational error can be eliminated. The translational error can be compensated by the image registration process. The z direction registration is performed by a least-squares error algorithm and the (x, y, z) translational information is determined. Finally, the overlapped regions of the measurement datasets are fused together by the edge intensity data fusion method. As a result, a large measurement area with a high resolution is obtained. A simulated and an actual measurement with a coherence scanning interferometer have been conducted to verify the proposed method. The stitching result shows that the proposed method is technically feasible for large area surface measurement

The Effect of Nitrided Layer on Antibacterial Properties for Biomedical Stainless Steel

AbstractPlasma nitriding of AISI type 303 austenitic stainless steel using microwave system at various input powers was conducted in present study. The nitrided layers were characterized via scanning electron microscopy, transmission electron microscopy and Vickers microhardness tester. The anti-bacterial property of this nitrided layer was also evaluated. The analytical results revealed the hardness of AISI type 303 stainless steel could be enhanced with nitriding process. The microstructure of the nitrided layer comprised of nitrogen-expanded γ phase. Bacterial test demonstrated the nitrided layer processed the excellent an ti-bacterial properties. The enhanced hardness and anti-bacterial properties make the nitrided AISI type 303 austenitic stainless steel the potential material in the biomedical applications

Estimation of Canadian manure and fertilizer nitrogen application rates for crops at the soil polygon level using the CANB v2.0 model

Non-Peer ReviewedIn response to national environmental and climate change modeling projects such as agri-environmental indicators, greenhouse gases, carbon sequestration and policy scenarios, fertilizer N and manure nitrogen N application rates were estimated for individual crops at the Soil Landscapes of Canada (SLC) polygon scale (1:1 million). This database provides an estimate of the actual amount of N applied per crop and per hectare, based on provincial fertilization recommendations, manure production levels of each type of livestock and reported amounts of fertilizer sold. The database is being incorporated into ongoing programs related to Kyoto accounting of greenhouse gas emissions, environmental performance and policy formulation at Agriculture and Agri-Food Canada. A standardized Canadian Agricultural Nitrogen Budget (CANB v2.0) model was developed to calculate the agri-environmental indicators Residual Soil Nitrogen (RSN) and Indicator of Risk of Water Contamination by Nitrogen (IROWC-N). CANB is a national-level model that operates on 3500 SLC polygons using generalized soil, landscape, climate, and Census of Agriculture socioeconomic data. It is designed to provide a regional update on the soil N balance for each of the census years of 1981, 1986, 1991, 1996, 2001 and into the future. The database and model have the capability to calculate a number of different components of the nutrient balance, including the inputs of fertilizer N, manure N, biological N and atmospheric N and N the removals of N in the harvested proportion of the crop and via nitrogenous gas emissions. This paper describes the procedures to estimate fertilizer N and manure N inputs for each crop within each polygon. It includes: (i) the compilation of soil-specific N application rates from provincial extension guidelines, (ii) the calculation of total manure N production from animal numbers and excretion rates, (iii) the calculation of available manure N after storage and handling losses, and (iv) the recommended and adjusted nitrogen application rates. Adjustments were made to account for the amount of inorganic N in the manure applied to the various crops. The adjusted nitrogen rate data was also reconciled with the provincial fertilizer sales data

Effect of Chinese herbal medicines on rumen fermentation, methanogenesis and microbial flora in vitro

This study was carried out to evaluate the effects of three Chinese herbal medicines (CHMs) on rumen fermentation, methane emission and population of ruminal microbes using an in vitro gas production technique. Three healthy wethers of Dazu Black goats with similar bodyweights and permanent rumen fistulae were utilized as donors of ruminal fluid. The three botanical medicines were cablin patchouli herb (CPH), atractylodes rhizome (AR) and Amur cork tree (AC). Each CHM was added at a level of 25 g/kg to the substrate dry matter. In vitro gas production was recorded, and methane concentration was determined at 12 and 24 hours of incubation. After 24 hours, the incubation was stopped, and the inoculants were measured for pH, ammonia nitrogen and volatile fatty acids (VFAs) concentrations. Total deoxyribonucleic acid of ruminal microbes was extracted from the inocula, and populations were determined by a real-time quantitative polymerase chain reaction. Populations of total rumen methanogens, protozoa, total fungi, Ruminococcus albus, Fibrobacter succinogenes and Ruminococcus flavefaciens were expressed as a proportion of total rumen bacterial 16S ribosomal deoxyribonucleic acid. Compared to the control, CPH decreased gas production and methane production at 12 and 24 hours of incubation, and inhibited methanogens and total fungi growth. AR decreased acetate to propionate ratio, and methanogens and total fungi populations, but increased propionate molar proportion. AC decreased total VFA concentration, acetate to propionate ratio, gas production at 12 and 24 hours of incubation, methane production at 12 and 24 hours of incubation, and methanogens and total fungi growth, but increased the propionate molar proportion. In conclusion, CPH and AC both suppressed methanogenesis significantly, and the suppression was mediated primarily via the direct action against the rumen microbes involved in methane formation. AC also indirectly abates methane release by occupying the hydrogen (H2) normally utilized for methanogenesis.Keywords: In vitro gas production, methane, rumen fermentation parameter, rumen microbe

Responses of chemical composition, amino acid and fatty acid profiles of breast muscle to dietary crude fibre levels in China Micro-Ducks

The effects of dietary crude fibre (CF) levels on the chemical composition, amino acid (AA) and fatty acid (FA) profiles of breast muscle in male China Micro-ducks (21 - 56 d) were investigated in this study. The birds were randomly allocated to four treatments, of which dietary CF levels were 16.7, 42.6, 77.9 and 101.6 g/kg of dry matter (DM), respectively. No influences on the proportions of crude ash, crude protein, lysine, methionine, threonine, phenylalanine, histidine, arginine, aspartic acid, glutamic acid, glycine, alanine, serine, cysteine, tyrosine, proline, C12:0, C18:0, C18:1n9, C20:0 and C22:1n9 in muscle were observed across the treatments. Consequently, the concentrations of semi-essential AA, non-essential AA, total AA, aromatic AA and delicious AA in muscle, along with the Δ-9-desaturase (18) index, were not affected by the treatments. Significant lower concentrations of valine, leucine, isoleucine, essential AA, branched-chain AA, C16:1n7 and monounsaturated FA in muscle, along with a lower ratio of branched-chain to aromatic AA, were noted in a dietary treatment with a CF level of 16.7 g/kg DM compared to other dietary treatments. The rise of the dietary CF level significantly increased the proportions of DM, ether extract, gross energy, C18:2n6, C18:3n3, C20:4n6, unsaturated FA, total FA, polyunsaturated FA, n-3 polyunsaturated FA, n-6 polyunsaturated FA, essential FA, and the Δ-9-desaturase (16) index. Furthermore, the rise of the dietary CF level increased the ratios of unsaturated to saturated FA, polyunsaturated to saturated FA, and n-6 to n-3 polyunsaturated FA in muscle. However, it decreased the atherogenic index and the proportions of C14:0, C16:0, and saturated FA significantly. In conclusion, depending largely on dietary levels, CF profoundly and positively influenced the nutritional quality of breast muscle, especially the FA profile.Keywords: China Micro-duck, dietary fibre, fatty acid, nutritional quality of meat, fatty aci

Quantitative evaluation of motor function before and after engraftment of dopaminergic neurons in a rat model of Parkinson's disease

Although gait change is considered a useful indicator of severity in animal models of Parkinson's disease, systematic and extensive gait analysis in animal models of neurological deficits is not well established. The CatWalk-assisted automated gait analysis system provides a comprehensive way to assess a number of dynamic and static gait parameters simultaneously. In this study, we used the Catwalk system to investigate changes in gait parameters in adult rats with unilateral 6-OHDA-induced lesions and the rescue effect of dopaminergic neuron transplantation on gait function. Four weeks after 6-OHDA injection, the intensity and maximal area of contact were significantly decreased in the affected paws and the swing speed significantly decreased in all four paws. The relative distance between the hind paws also increased, suggesting that animals with unilateral 6-OHDA-induced lesions required all four paws to compensate for loss of balance function. At 8 weeks post-transplantation, engrafted dopaminergic neurons expressed tyrosine hydroxylase. In addition, the intensity, contact area, and swing speed of the four limbs increased and the distance between the hind paws decreased. Partial recovery of methamphetamine-induced rotational response was also noted