Epidemiology of canine distemper in Makurdi, Nigeria

Canine distemper (CD), a major disease of dogs all over the world, hitherto controlled by extensive vaccination of dogs around the world, appears to be persisting and even re-emerging in many parts of the world. It is thought that wild life reservoir hosts contribute to the emergence of CD, but in areas of the world with minimal wildlife contact with domestic dogs, it is thought that cyclical infection between clinically normal dogs and susceptible neonatal animals may be responsible for maintaining the canine distemper virus (CDV) in the canine population. We decided to examine clinically normal dogs in the Makurdi metropolis for evidence of infection with CDV, to determine if such dogs may act as sources of persistence of the CDV in the canine population. We tested 70 unvaccinated, clinically normal dogs for evidence of distemper virus using a rapid CDV antigen (Ag) chromatographic assay test kit designed for the qualitative detection of canine distemper virus antigens in urine, conjunctiva, serum or plasma. We found six (6 or 8.6%) of the 70 dogs positive for distemper antigen; three (3) of the dogs were under one (1) year of age, whereas three were 5 years or more. We conclude that the CDV is circulating among clinically normal dogs in Makurdi, and that a cyclical infection between infected but clinically normal adult dogs and puppies may be responsible for maintaining the disease in the canine population in Makurdi. Further studies are necessary to elucidate the role of vaccination and the possibilities of emergence of new antigenic strains of CDV in the epidemiology of CD in the Makurdi area.Keywords: Canine Distemper, Vaccination, Laboratory diagnosis, maintenance in population, Makurdi, Nigeri

Incorporation of Dry <em>Mangifera indica</em> Kernel in the Concentrate Ration of Growing Lambs

Twenty Southeast Dwarf yearling lambs of Nigeria were grouped according to their live weight (20.9 ± 1.7 kg) and offered one of the five concentrate rations as a supplement to the low quality dry season forage of mixed grass/ legume hays (mainly three part Pennisetum purpureum and one part Centrosema pubescens) for 56 days growth period, after which five of the lambs were relocated to metabolism cages for digestibility and nitrogen balance trials. The Mangifera indica kernel was substituted for maize offal in diets A, B, C, D and E at 0, 15, 30, 45 and 60%, respectively. The concentrate intakes and growth rates were 951, 961, 1017, 1225 and 1001, and 116, 125, 146, 162 and 116 g/day, respectively

The VP2 variable region of African and German isolates of infectious bursal disease virus: comparison with very virulent, &quot;classical&quot; virulent, and attenuated tissue culture-adapted strains.

&lt;p&gt;11 African and two German IBDV strains isolated in the mid &#039;80s from field outbreaks in vaccinated and unvaccinated chicken flocks displayed features of very virulent (vv) IBDV strains. The sequence data of the VP2 variable region and phylogenetic analysis confirm that these strains can be grouped within vv IBDV strains which appeared at the same time on the three continents Africa, Asia, and Europe. Strain Cu-1wt, responsible for severe IBD outbreaks in Germany 13 years earlier, showed some relatedness to these strains, but also significant differences at the genomic level, even though this strain has also features of the vv IBDV strains.&lt;/p&gt;</p

Development and use of real-time PCR to detect and quantify Mycoplasma haemocanis and "Candidatus Mycoplasma haematoparvum" in dogs

Two canine haemoplasma species have been recognised to date; Mycoplasma haemocanis (Mhc), which has been associated with anaemia in splenectomised or immunocompromised dogs, and "Candidatus Mycoplasma haematoparvum" (CMhp), recently described in an anaemic splenectomised dog undergoing chemotherapy. The study aim was to develop quantitative real-time PCR assays (qPCRs) incorporating an endogenous internal control to detect Mhc and CMhp and to apply these assays to DNA samples extracted from canine blood collected in Northern Tanzania (n = 100) and from dogs presented to a Trinidadian veterinary hospital (n = 185). QPCRs specific for Mhc and CMhp were designed using 16S rRNA gene sequence data, and each was duplexed with an assay specific for canine glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The assays detected &lt;= 10 copies of a sequence-specific haemoplasma plasmid per reaction and neither assay showed cross-reactivity with 10(6) copies of the sequence-specific plasmid from the non-target canine haemoplasma species. Nineteen of the 100 Tanzanian samples (19%) were positive for Mhc alone and one (1%) was dually infected. One Trinidadian sample was negative for canine GAPDH DNA and was excluded from the study. Of the 184 remaining Trinidadian samples, nine (4.9%) were positive for Mhc alone, five (2.7%) for CMhp alone, and two (1.1%) dually infected. This is the first report of canine haemoplasma qPCR assays that use an internal control to confirm the presence of amplifiable sample DNA, and their application to prevalence studies. Mhc was the most commonly detected canine haemoplasma species