Experimental Investigation on the Aerodynamic Interaction Between a Helicopter and Ground Obstacles

In this study, experiments were performed to investigate the aerodynamic interaction between a helicopter and ground obstacles. A new experimental set-up was realised and validated. The motorised helicopter model, which included the fuselage, was positioned in different positions relative to a model building in order to replicate different hovering configurations. The use of a helicopter model with a six-component balance and a building model with several pressure taps allowed a database to be compiled for the loads on the helicopter and obstacle. First several tests were performed without the building in order to develop a reference database and assess the experimental set-up through a comparison with results in the literature. The measured loads were analysed to investigate the interference effects of the building model on the helicopter performance. A physical interpretation of the flow phenomena was obtained through analysis of the obstacle pressure measurements and particle image velocimetry surveys of relevant configurations

The adherent/invasive escherichia coli (AIEC) strain LF82 invades and persists in human prostate cell lineRWPE-1 activating a strong inflammatory response

Adherent/invasive Escherichia coli (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic E. coli (ExPEC), neonatal meningitis-associated E. coli (NMEC), and uropathogenic E. coli (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect prostate cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 prostate cell line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within prostate epithelial cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers. In silico analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-κB already by 5 min postinfection, thus inducing a strong inflammatory response. Our in vitro data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary trac

Co-optimizing grating couplers for hybrid integration of InP and SOI photonic platforms

Grating couplers are widely used optical interfaces in integrated photonics, especially on the Silicon-On-Insulator (SOI) platform. Their design has been optimized for coupling light between a Photonic Integrated Circuit (PIC) and a single-mode fiber, a μlens for free space transport, or even a second PIC in the same SOI platform. In this work, we co-design matching pairs of grating-couplers on distinct SOI and InP photonic platforms for optimized PIC-to-PIC coupling. By matching the scattering strengths of the two grating-couplers, we show that a PIC-to-PIC insertion loss of 3dB can be achieved. We also investigate how the design parameters impact the coupling efficiency and the bandwidth, ending up with a tolerance analysis. The proposed coupling approach between two different waveguide materials has prospective applications for the hybrid-integration of SOI and InP photonic platforms for communication technologies.Grating couplers are widely used optical interfaces in integrated photonics, especially on the Silicon-On-Insulator (SOI) platform. Their design has been optimized for coupling light between a Photonic Integrated Circuit (PIC) and a single-mode fiber, a μlens for free space transport, or even a second PIC in the same SOI platform. In this work, we co-design matching pairs of grating-couplers on distinct SOI and InP photonic platforms for optimized PIC-to-PIC coupling. By matching the scattering strengths of the two grating-couplers, we show that a PIC-to-PIC insertion loss of 3dB can be achieved. We also investigate how the design parameters impact the coupling efficiency and the bandwidth, ending up with a tolerance analysis. The proposed coupling approach between two different waveguide materials has prospective applications for the hybrid-integration of SOI and InP photonic platforms for communication technologies

Chlamydia pneumoniae in asymptomatic carotid atherosclerosis.

We evaluated, in 415 patients with asymptomatic carotid atherosclerosis: (i) the prevalence of C. pneumoniae DNA in atherosclerotic carotid plaques and peripheral blood mononuclear cells (PBMC); (i..

Nanoemulsions of Satureja montana essential oil. Antimicrobial and antibiofilm activity against avian Escherichia coli strains

Satureja montana essential oil (SEO) presents a wide range of biological activities due to its high content of active phytochemicals. In order to improve the essential oil’s (EO) properties, oil in water nanoemulsions (NEs) composed of SEO and Tween-80 were prepared, characterized, and their antimicrobial and antibiofilm properties assayed against Escherichia coli strains isolated from healthy chicken. Since surfactant and oil composition can strongly influence NE features and their application field, a ternary phase diagram was constructed and evaluated to select a suitable sur-factant/oil/water ratio. Minimal inhibitory concentration and minimal bactericidal concentration of NEs, evaluated by the microdilution method, showed that the SEO NE formulation exhibited higher inhibitory effects against planktonic E. coli than SEO alone. The quantification of biofilm production in the presence of NEs, assessed by crystal violet staining and scanning electron microscopy, evi-denced that sub-MIC concentrations of SEO NEs enable an efficient reduction of biofilm production by the strong producer strains. The optimized nanoemulsion formulation could ensure food safety quality, and counteract the antibiotic resistance of poultry associated E. coli, if applied/aerosolized in poultry farms

Cardiac investigations in sudden unexpected death in DEPDC5-related epilepsy

Objective: Germline loss-of-function mutations in DEPDC5, and in its binding partners (NPRL2/3) of the mammalian target of rapamycin (mTOR) repressor GATOR1 complex, cause focal epilepsies and increase the risk of sudden unexpected death in epilepsy (SUDEP). Here, we asked whether DEPDC5 haploinsufficiency predisposes to primary cardiac defects that could contribute to SUDEP and therefore impact the clinical management of patients at high risk of SUDEP. Methods: Clinical cardiac investigations were performed in 16 patients with pathogenic variants in DEPDC5, NPRL2, or NPRL3. Two novel Depdc5 mouse strains, a human HA-tagged Depdc5 strain and a Depdc5 heterozygous knockout with a neuron-specific deletion of the second allele (Depdc5c/−), were generated to investigate the role of Depdc5 in SUDEP and cardiac activity during seizures. Results: Holter, echocardiographic, and electrocardiographic (ECG) examinations provided no evidence for altered clinical cardiac function in the patient cohort, of whom 3 DEPDC5 patients succumbed to SUDEP and 6 had a family history of SUDEP. There was no cardiac injury at autopsy in a postmortem DEPDC5 SUDEP case. The HA-tagged Depdc5 mouse revealed expression of Depdc5 in the brain, heart, and lungs. Simultaneous electroencephalographic–ECG records on Depdc5c/− mice showed that spontaneous epileptic seizures resulting in a SUDEP-like event are not preceded by cardiac arrhythmia. Interpretation: Mouse and human data show neither structural nor functional cardiac damage that might underlie a primary contribution to SUDEP in the spectrum of DEPDC5-related epilepsies. ANN NEUROL 2022;91:101–11

Co-optimizing grating couplers for hybrid integration of InP and SOI photonic platforms

Grating couplers are widely used optical interfaces in integrated photonics, especially on the Silicon-On-Insulator (SOI) platform. Their design has been optimized for coupling light between a Photonic Integrated Circuit (PIC) and a single-mode fiber, a µlens for free space transport, or even a second PIC in the same SOI platform. In this work, we co-design matching pairs of grating-couplers on distinct SOI and InP photonic platforms for optimized PIC-to-PIC coupling. By matching the scattering strengths of the two grating-couplers, we show that a PIC-to-PIC insertion loss of 3dB can be achieved. We also investigate how the design parameters impact the coupling efficiency and the bandwidth, ending up with a tolerance analysis. The proposed coupling approach between two different waveguide materials has prospective applications for the hybrid-integration of SOI and InP photonic platforms for communication technologies