Active primate simulator Final report

Systems engineering data and design specifications for Biosatellite active primate simulato

Sustainable gardens: an evaluation tool for management and planning strategies.

An evaluation tool was defined in order to manage gardens considering the six main directives of sustainability, from plant collections to public awareness and fruition

Osservazioni Citotassonomiche Su Scilla Barbacaprae, Endemismo Cirenaico, E Sue Affinita' Con Scilla Villosa

RIASSUNTOViene analizzato il cariotipo di Scilla barba caprae, specie endemica di Derna (Cirenaica settentrionale). Il risultato di questa indagine, effettuata su bulbi coltivati nell'Orto Botanico di Catania, e stato il rinvenimento di un corredo cromosomico diploide: z = 2n = 2x = 14 = 2L + 2M1 + 2M2 + 2M3S3 + 2M4 + 2S1 + 2S2.La comparazione fra Scilla barba-caprae e Scilla villosa, che e la specie tassonomicamente piu vicina, ha permesso di evidenziare numerose differenze riguardanti sia il cariotipo che il fenotipo, che consentono di separare le due entita a livello specifico.In particolare il cariotipo di Scilla villosa (2n = 20) differisce da quello fondamentale 2n= 16, relativo alle entita appartenenti al ciclo di Scilla peruviana, per avere in piu quattro cromosomi, mentre quello di Scilla barba-caprae (2n = 14) ne differisce per avere due cromosomi in meno e per la presenza della coppia 2M3S3 formatasi in seguito a fenomeni di traslocazione + delezione

Seed morphology and seed coat structure in Mediterranean plants: implication for evolution, taxonomy and germinative performance

A better understanding of morphological, ultra-structural and anatomical seed coat characteristics can contribute to explain and predict seed performance under certain environmental conditions and select the most suitable treatments promoting seed germination. The Seed Banks at the Botanic Gardens of Catania and Palermo (Sicily) are particularly involved in research studies aimed to detect and describe macro- and micro-morphological features of seeds from different plant families and genera, using both light and scanning electron microscopy. These studies mainly aim to 1) recognize and compare inter- and intra-specific variation of seed coat traits, with particular attention on rare and threatened species; 2) evaluate the usefulness of these traits in taxonomic and phylogenetic studies; 3) establish correlations between seed coat structures and seed germination; 4) understand the seed coat adaptive potential and sensitivity to particular ecological conditions and changing environments

transferable drug resistance in escherichia coli isolated from antibiotic fed chickens

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Optical mapping of neuronal activity during seizures in zebrafish

Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs. Recently, zebrafish has become an important model for studying epilepsy both in basic research and in drug discovery. Here, we employed a transgenic line with pan-neuronal expression of the genetically-encoded calcium indicator GCaMP6s to measure neuronal activity in zebrafish larvae during seizures induced by pentylenetretrazole (PTZ). With this approach, we mapped neuronal activity in different areas of the larval brain, demonstrating the high sensitivity of this method to different levels of alteration, as induced by increasing PTZ concentrations, and the rescuing effect of an anti-epileptic drug. We also present simultaneous measurements of brain and locomotor activity, as well as a high-throughput assay, demonstrating that GCaMP measurements can complement behavioural assays for the detection of subclinical epileptic seizures, thus enabling future investigations on human hypomorphic mutations and more effective drug screening methods. Notably, the methodology described here can be easily applied to the study of many human neuropathologies modelled in zebrafish, allowing a simple and yet detailed investigation of brain activity alterations associated with the pathological phenotype

Preterm birth after loop electrosurgical excision procedure (LEEP). how cone features and microbiota could influence the pregnancy outcome

OBJECTIVE: In the last years, the mean age of women who underwent cervical treatment for high-grade cervical intraepithelial neoplasia (CIN 2-3) is similar to the age of women having their first pregnancy. The aim of this study was to evaluate the risk of preterm birth in subsequent pregnancies after loop electrosurgical excision procedure (LEEP). PATIENTS AND METHODS: From January 2013 to January 2016 the study identified a total of 1435 women, nulliparous, who underwent LEEP for CIN 2-3, and who wished to have their first pregnancy. Before surgery, the lengths of the cervix were calculated by transvaginal sonography. After the treatment, the dimension of the removed tissue was evaluated. During the pregnancy, all women carried out periodic transvaginal sonography and vaginal-cervical swabs. RESULTS: The average age of patients was 31.96±5.24 years; the interval between the surgical procedure and pregnancy was 12.04±4.67 months; the gestational age at births was 37.53±2.91 weeks. The first vaginal and cervical swab performed during pregnancy was negative in 81.8% of patients. The most prevalent infections were related to C. Albicans, G. Vaginalis, and Group B Streptococcus (GBS). The rate of preterm delivery was significantly higher in women with a minor cervical length. CONCLUSIONS: The length and the volume of cervical tissue excised have been shown to be directly related to the risk for preterm birth. Furthermore, vaginal infections and their persistence during pregnancy in women with a history of LEEP may be associated with an increased risk for preterm birth, compared with women with no history of LEEP

Real-time optical manipulation of cardiac conduction in intact hearts

Optogenetics has provided new insights in cardiovascular research, leading to new methods for cardiac pacing, resynchronization therapy and cardioversion. Although these interventions have clearly demonstrated the feasibility of cardiac manipulation, current optical stimulation strategies do not take into account cardiac wave dynamics in real time. Here, we developed an all‐optical platform complemented by integrated, newly developed software to monitor and control electrical activity in intact mouse hearts. The system combined a wide‐field mesoscope with a digital projector for optogenetic activation. Cardiac functionality could be manipulated either in free‐run mode with submillisecond temporal resolution or in a closed‐loop fashion: a tailored hardware and software platform allowed real‐time intervention capable of reacting within 2 ms. The methodology was applied to restore normal electrical activity after atrioventricular block, by triggering the ventricle in response to optically mapped atrial activity with appropriate timing. Real‐time intraventricular manipulation of the propagating electrical wavefront was also demonstrated, opening the prospect for real‐time resynchronization therapy and cardiac defibrillation. Furthermore, the closed‐loop approach was applied to simulate a re‐entrant circuit across the ventricle demonstrating the capability of our system to manipulate heart conduction with high versatility even in arrhythmogenic conditions. The development of this innovative optical methodology provides the first proof‐of‐concept that a real‐time optically based stimulation can control cardiac rhythm in normal and abnormal conditions, promising a new approach for the investigation of the (patho)physiology of the heart