10 research outputs found

    Alteration of the embryo transcriptome of hexaploid winter wheat (Triticum aestivum cv. Mercia) during maturation and germination

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    Grain dormancy and germination are areas of biology that are of considerable interest to the cereal community. We have used a 9,155-feature wheat unigene cDNA microarray resource to investigate changes in the wheat embryo transcriptome during late grain development and maturation and during the first 48 h of postimbibition germination. In the embryo 392 mRNAs accumulated by twofold or greater over the time course from 21 days postanthesis (dpa) to 40 dpa and on through 1 and 2 days postgermination. These included mRNAs encoding proteins involved in amino acid biosynthesis and metabolism, cell division and subsequent cell development, signal transduction, lipid metabolism, energy production, protein turnover, respiration, initiation of transcription, initiation of translation and ribosomal composition. A number of mRNAs encoding proteins of unknown function also accumulated over the time course. Conversely 163 sequences showed decreases of twofold or greater over the time course. A small number of mRNAs also showed rapid accumulation specifically during the first 48 h of germination. We also examined alterations in the accumulation of transcripts encoding proteins involved in abscisic acid signalling. Thus, we describe changes in the level of transcripts encoding wheat Viviparous 1 (Vp1) and other interacting proteins. Interestingly, the transcript encoding wheat Viviparous-interacting protein 1 showed a pattern of accumulation that correlates inversely with germination. Our data suggests that the majority of the transcripts required for germination accumulate in the embryo prior to germination and we discuss the implications of these findings with regard to manipulation of germination in wheat

    Application of a telereference system to divisional library card catalogs : a feasibility analysis : final report

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    http://deepblue.lib.umich.edu/bitstream/2027.42/3351/5/bab2613.0001.001.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/3351/4/bab2613.0001.001.tx

    An audio-based personal memory aid

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    We are developing a wearable device that attempts to alleviate some everyday memory problems. The “memory prosthesis” records audio and contextual information from conversations and provides a suite of retrieval tools (on both the wearable and a personal computer) to help users access forgotten memories in a timely fashion. This paper describes the wearable device, the personal-computer-based retrieval tool, and their supporting technologies. Anecdotal observations based on real-world use and quantitative results based on a controlled memory-retrieval task are reported. Finally, some social, legal, and design challenges of ubiquitous recording and remembering via a personal audio archive are discussed

    Privacy-Enhanced Web Personalization

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    Abstract. Consumer studies demonstrate that online users value personalized content. At the same time, providing personalization on websites seems quite profitable for web vendors. This win-win situation is however marred by privacy concerns since personalizing people's interaction entails gathering considerable amounts of data about them. As numerous recent surveys have consistently demonstrated, computer users are very concerned about their privacy on the Internet. Moreover, the collection of personal data is also subject to legal regulations in many countries and states. Both user concerns and privacy regulations impact frequently used personalization methods. This article analyzes the tension between personalization and privacy, and presents approaches to reconcile the both. It has been tacitly acknowledged for many years that personalized interaction and user modeling have significant privacy implications, due to the fact that large amounts of personal information about users needs to be collected to perform personalization. Fo
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