Swine zoonosis risk assessment with new herd seroprofiling assays from QIAGEN

QIAGEN Leipzig developed the pigtype product line of ELISA tests for screening for swine zoonoses. This product line now includes ELISA for detection of salmonella-, Yersinia-, Trichinella-, and Toxoplasma-antibodies in swine. These pigtype assays are validated for serum and meat juice samples and are officially approved by the German Friedrich-Loeffler-Institut

CAPOEIRA E EXTENSÃO UNIVERSITÁRIA: PERCURSOS, DIÁLOGOS E DESAFIOS

This article presented an experience report regarding an Extension project developed at the State University of Minas Gerais - UEMG since 2018. For organization purposes, the text is divided into three equally important ones. The first will deal, succinctly, with impressions and subjective experiences with capoeira. In this part, in addition to the relevant academic knowledge, the author's route as a master of the art will be presented. In the following part, a brief analysis of the various conceptions of university extension will be carried out. Finally, in the third part, a narrative will be treated about the author's experience as project coordinator for more than five years at UEMG.Este artículo presentó un relato de experiencia sobre un proyecto de Extensión desarrollado en la Universidad del Estado de Minas Gerais - UEMG desde 2018. Para fines de organización, el texto se divide en tres igualmente importantes. El primero tratará, sucintamente, de impresiones y experiencias subjetivas con la capoeira. En esta parte, además de los conocimientos académicos pertinentes, se presentará el recorrido del autor como maestro del arte. En la siguiente parte se realizará un breve análisis de las diversas concepciones de la extensión universitaria. Finalmente, en la tercera parte, se tratará una narración sobre la experiencia del autor como coordinador de proyectos durante más de cinco años en la UEMG.Neste artigo apresentamos um relato de experiência a respeito de um projeto de Extensão desenvolvido na Universidade do Estado de Minas Gerais – UEMG desde o ano de 2018. Para fins de organização, o texto está dividido em três partes igualmente importantes. A primeira tratará, de forma sucinta, das impressões e experiências subjetivas com a capoeira. Nessa parte, além dos saberes acadêmicos pertinentes, será apresentada a trajetória do autor como mestre da arte. Na parte seguinte, será realzada uma breve análise das diversas concepções de extensão universitária. Por fim, na terceira parte, será tratada uma narrativa a respeito dessa experiência do autor como coordenador do projeto por mais de cinco anos, na Universidade do Estado de Minas Gerais - UEMG

Hepatic gene expression involved in glucose and lipid metabolism in transition cows: effects of fat mobilization during early lactation in relation to milk performance and metabolic changes.

Insufficient feed intake during early lactation results in elevated body fat mobilization to meet energy demands for milk production. Hepatic energy metabolism is involved by increasing endogenous glucose production and hepatic glucose output for milk synthesis and by adaptation of postcalving fuel oxidation. Given that cows differ in their degree of fat mobilization around parturition, indicated by variable total liver fat concentration (LFC), the study investigated the influence of peripartum fat mobilization on hepatic gene expression involved in gluconeogenesis, fatty acid oxidation, ketogenesis, and cholesterol synthesis, as well as transcriptional factors referring to energy metabolism. German Holstein cows were grouped according to mean total LFC on d 1, 14, and 28 after parturition as low [300 mg of total fat/g of DM; n=7), indicating fat mobilization during early lactation. Cows were fed total mixed rations ad libitum and held under equal conditions. Liver biopsies were taken at d 56 and 15 before and d 1, 14, 28, and 49 after parturition to measure mRNA abundances of pyruvate carboxylase (PC); phosphoenolpyruvate carboxykinase; glucose-6-phosphatase; propionyl-coenzyme A (CoA) carboxylase α; carnitine palmitoyl-transferase 1A (CPT1A); acyl-CoA synthetase, long chain 1 (ASCL1); acyl-CoA dehydrogenase, very long chain; 3-hydroxy-3-methylglutaryl-CoA synthase 1 and 2; sterol regulatory element-binding factor 1; and peroxisome proliferator-activated factor α. Total LFC postpartum differed greatly among cows, and the mRNA abundance of most enzymes and transcription factors changed with time during the experimental period. Abundance of PC mRNA increased at parturition to a greater extent in high- and medium-LFC groups than in the low-LFC group. Significant LFC × time interactions for ACSL1 and CPT1A during the experimental period indicated variable gene expression depending on LFC after parturition. Correlations between hepatic gene expression and performance data and plasma concentrations of metabolites and hormones showed time-specific relations during the transition period. Elevated body fat mobilization during early lactation affected gene expression involved in gluconeogenesis to a greater extent than gene expression involved in lipid metabolism, indicating the dependence of hepatic glucose metabolism on hepatic lipid status and fat mobilization during early lactation

Surface matters: Limitations of CALIPSO V3 aerosol typing in coastal regions

In the CALIPSO data analysis, surface type (land/ocean) is used to augment the aerosol characterization. However, this surface-dependent aerosol typing prohibits a correct classification of marine aerosol over land that is advected from ocean to land. This might result in a systematic overestimation of the particle extinction coefficient and of the aerosol optical thickness (AOT) of up to a factor of 3.5 over land in coastal areas. We present a long-term comparison of CALIPSO and ground-based lidar observations of the aerosol conditions in the coastal environment of southern South America (Punta Arenas, Chile, 53° S), performed in December 2009–April 2010. Punta Arenas is almost entirely influenced by marine particles throughout the year, indicated by a rather low AOT of 0.02–0.04. However, we found an unexpectedly high fraction of continental aerosol in the aerosol types inferred by means of CALIOP observations and, correspondingly, too high values of particle extinction. Similar features of the CALIOP data analysis are presented for four other coastal areas around the world. Since CALIOP data serve as important input for global climate models, the influence of this systematic error was estimated by means of simplified radiative-transfer calculations

Mobilizing professors’ support of digital change: Multi-level insights on IT resources as a boundary condition

The success of top-down digital change initiatives in higher education institutions (HEIs) largely depends on the support of professors as change recipients and catalysts within their departments. For effectively managing change, a better understanding of how process factors under management control (i.e., vision communication, change facilitation, participation opportunities, change coordination) simultaneously relate to professors’ cognitive and behavioral change support is crucial. Moreover, we examine how department-level IT resources as a context factor shape process-reaction relationships. Based on data from 1,400 professors nested in 258 departments within German HEIs, multilevel regression analyses support the relevance of vision communication, change facilitation, and participation opportunities — but not of change coordination. As department-level IT resources increase, vision communication more strongly relates to cognitive change support, pointing to unexplored higher-level boundary conditions of vision communication. Our study advances knowledge about mobilizing change support and managing top-down change with limited top-down influence to impose change

Intrauterine Growth Retarded Progeny of Pregnant Sows Fed High Protein:Low Carbohydrate Diet Is Related to Metabolic Energy Deficit

High and low protein diets fed to pregnant adolescent sows led to intrauterine growth retardation (IUGR). To explore underlying mechanisms, sow plasma metabolite and hormone concentrations were analyzed during different pregnancy stages and correlated with litter weight (LW) at birth, sow body weight and back fat thickness. Sows were fed diets with low (6.5%, LP), adequate (12.1%, AP), and high (30%, HP) protein levels, made isoenergetic by adjusted carbohydrate content. At −5, 24, 66, and 108 days post coitum (dpc) fasted blood was collected. At 92 dpc, diurnal metabolic profiles were determined. Fasted serum urea and plasma glucagon were higher due to the HP diet. High density lipoprotein cholesterol (HDLC), %HDLC and cortisol were reduced in HP compared with AP sows. Lowest concentrations were observed for serum urea and protein, plasma insulin-like growth factor-I, low density lipoprotein cholesterol, and progesterone in LP compared with AP and HP sows. Fasted plasma glucose, insulin and leptin concentrations were unchanged. Diurnal metabolic profiles showed lower glucose in HP sows whereas non-esterified fatty acids (NEFA) concentrations were higher in HP compared with AP and LP sows. In HP and LP sows, urea concentrations were 300% and 60% of AP sows, respectively. Plasma total cholesterol was higher in LP than in AP and HP sows. In AP sows, LW correlated positively with insulin and insulin/glucose and negatively with glucagon/insulin at 66 dpc, whereas in HP sows LW associated positively with NEFA. In conclusion, IUGR in sows fed high protein∶low carbohydrate diet was probably due to glucose and energy deficit whereas in sows with low protein∶high carbohydrate diet it was possibly a response to a deficit of indispensable amino acids which impaired lipoprotein metabolism and favored maternal lipid disposal

Health-Related Physical Fitness in Female Models

Abstract The model profession uses the appearance for the representation of products and brands via events. For some individuals that are included in this medium, plus a laboral activity, modeling becomes a lifestyle, the search for the status and work opportunities turn a dream for a thousands of children and teenagers because the profession has particulars experiences. To win this, many girls change physical and eating behaviors which are harmful to health. The objective of this study was to analyze the health-related physical fitness in female models comparing them with nonmodels. The study was conducted at the Caxias do Sul, Rio Grande do Sul, Brazil. Participated of the study female runway and commercial models bokered in Cast One Models, with age between 15 -25 years old. The non-models were students from public and private schools or university students. The health-related physical fitness, the physical activity levels and dietary intake were assessed of all participants. The data were described as mean and standard deviation. For comparison between models and non-models was used t independent test for variables normally distributed and U Mann-Whitney test for not normally distributed variables, adopting a level of significance (α) of 0.05. It was found difference in total energy between model group and non-model (GM: 1509.78 kcal, NM: 2292.51 Kcal; p = 0.014). There were no differences between groups in the others variables analyzed (p > 0.05). In conclusion, the profession model seems not interfere in variables that make up the health-related physical fitness