Afterword: Why Kham? Why Borderlands? Coordinating New Research Programs for Asia

This special issue of Cross-Currents is dedicated to Kham, or Eastern Tibet, which, according to the European Research Council grant supporting these articles, can be called a “Sino-Tibetan Borderlands.” But why should East Asianists, including readers of this journal, care about Kham, and does it in any way help us to conceive of the region as a “borderlands”? The first question was on my mind in May 2015 as I participated in the first of two workshops devoted to Kham; the second was raised by rightfully skeptical participants—most of them experts on Kham—at the February 2016 conference in Paris that concluded this project. The two questions are related, I believe, and this afterword suggests that one possible answer to both lies in using local Kham history to push the boundaries of global borderlands studies. My goal is to argue for an approach that both frames the complexities of Kham for outsiders, including myself, and provides one (but certainly not the only) option for coordinating the diverse research agendas of Kham specialists..

"Le deuil du Tibet"

Cet article explore sous trois angles le processus de construction de l’État à la fin des Qing (1877-1911) en Asie intérieure (Xinjiang, Mongolie et Tibet). Il démontre que les efforts pour remplacer les institutions hybrides impériales par une administration de style chinois ont été des processus contingents et imprévisibles. Il compare les relations entre les élites et l’État, en Asie intérieure et en Chine, afin de déterminer les différents impacts sur les élites mongoles, tibétaines et han. Enfin, il étudie les médias de la période des réformes (1898-1911) pour révéler la nouvelle conception par les élites han des territoires d’Asie intérieure et de leurs peuples, ainsi que les conséquences durables de ces changements

"Grieving for Tibet"

This article explores late Qing (1877-1911) state-building in Inner Asia (Xinjiang, Mongolia, and Tibet) in three ways. It demonstrates how efforts to replace hybrid, imperial institutions with Chinese-style administration were contingent and unpredictable processes. It compares elite-state relations, in Inner Asia and China proper, to explore the diverse impacts on Mongol, Tibetan, and Han elites. Finally, it surveys reform-era (1898-1911) media to reveal how Han elites conceived of Inner Asian territories and peoples in new ways and with enduring consequences

Integrated Nuclear Knowledge Management System – NUTEMA

Knowledge Management has become one of the most important issues for the nuclear industry. On the one hand, the amount of codifiable knowledge in the nuclear technology area saw a steep increase in the last years; on the other hand, due to the “generation gap” in the nuclear industry it became very challenging to organize the seamless transfer of the noncodifiable knowledge from one generation of engineers to the other. Computer aided systems so far where aiming at to preserve the codifiable knowledge. The present paper introduces a system that should support knowledge management not only dealing with the codifiable part of it, but also address broader aspects (which includes the management of non-codifiable knowledge).- NUTEMA. The integrated nuclear knowledge management system NUTEMA should provide interactive combination of information and methods, but also identifying competences which more adequately fit to a given task, keeping track of keeping skills of the experts within an organization. Application is foreseen in nuclear engineering fields as system design, operation and maintenance plant and process modifications, standardization, certification and even for licensing-related tasks. The system combines an extensively diverse and modular database with computer based simulations including a scientific software platform. NUTEMA is conceived to operate in different modes, for example collecting and retrieving database knowledge, training applications, NPP operations support, computer code applications, and as plant analyzer. This paper will present two examples; one acting as at a supporting tool for typical NPP plant modification: In a second case, application on review and optimization of operational process is described. Despite the provided examples deal with different objectives and methods associated with different stages of an NPP lifetime, (design and operation) both are supported by the integrated nuclear knowledge management system

Mild Dehydration Led to Increased Difficulty Falling Asleep

Sleep is fundamental process that benefits health and overall quality of life which can be affected by various aspects of daily living such as dehydration. A study has yet to investigate the impacts of euhydration and mild dehydration on sleep. PURPOSE: The purpose of this study was to examine effects of euhydration, mild-dehydration, and ad libitum drinking on sleep. METHODS: Eighteen male participants (mean±SD; age, 23±4y; height, 175.8±5.7cm; weight, 80.1±9.7kg) reported to the laboratory with different hydration status for 4 consecutive mornings(Day 1, baseline; Day 2, euhydrated; Day 3, mild-dehydrated; Day 4, ad libitum drinking). Hydration status was monitored by first morning urine specific gravity (USG) and plasma osmolality. Sleepwas measured using the Karolinska sleep diary (KSD). RESULTS: USG (baseline, 1.024±0.006; euhydrated, 1.018±0.007; mild-dehydrated, 1.030±0.003; ad libitum, 1.021±0.008, pCONCLUSION: When subjects were mildly dehydrated, sleep duration was longer while it was more difficult to fall asleep. More research is necessary, but the results of this study suggest it may be important to maintain euhydration to fall asleep easier

A re-investigation of the path of carbon in photosynthesis utilizing GC/MS methodology. Unequivocal verification of the participation of octulose phosphates in the pathway

A GC/EIMS/SIM methodology has been developed to re-examine the path of carbon in photosynthesis. Exposing isolated spinach chloroplasts to (13)CO(2 )on a solid support for a defined period followed by quenching and work-up provided a mixture of labelled sugar phosphates. After enzymatic dephosphorylation and derivatization, the Mox-TMS sugars were analysed using the above method. The purpose of the study was to try to calculate the atom% enrichment of (13)C in as many of the individual carbons in each of the derivatized sugars as was practical using diagnostic fragment ions. In the event, only one 45 s experiment provided sufficient data to enable a range of enrichment values to be calculated. This confirmed that D-glycero-D-altro-octulose phosphate was present in the chloroplasts and was heavily labelled in the C4, C5 and C6 positions, in keeping with the hypothesis that it had an inclusive role and a labelling pattern consistent with a new modified pathway of carbon in photosynthesis

CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains

Citation: Roggenkamp E, Giersch RM, Wedeman E, Eaton M, Turnquist E, Schrock MN, Alkotami L, Jirakittisonthon T, Schluter-Pascua SE, Bayne GH, Wasko C, Halloran M and Finnigan GC (2017) CRISPR-UnLOCK: Multipurpose Cas9-Based Strategies for Conversion of Yeast Libraries and Strains. Front. Microbiol. 8:1773. doi: 10.3389/fmicb.2017.01773Saccharomyces cerevisiae continues to serve as a powerful model system for both basic biological research and industrial application. The development of genome-wide collections of individually manipulated strains (libraries) has allowed for high-throughput genetic screens and an emerging global view of this single-celled Eukaryote. The success of strain construction has relied on the innate ability of budding yeast to accept foreign DNA and perform homologous recombination, allowing for efficient plasmid construction (in vivo) and integration of desired sequences into the genome. The development of molecular toolkits and “integration cassettes” have provided fungal systems with a collection of strategies for tagging, deleting, or over-expressing target genes; typically, these consist of a C-terminal tag (epitope or fluorescent protein), a universal terminator sequence, and a selectable marker cassette to allow for convenient screening. However, there are logistical and technical obstacles to using these traditional genetic modules for complex strain construction (manipulation of many genomic targets in a single cell) or for the generation of entire genome-wide libraries. The recent introduction of the CRISPR/Cas gene editing technology has provided a powerful methodology for multiplexed editing in many biological systems including yeast. We have developed four distinct uses of the CRISPR biotechnology to generate yeast strains that utilizes the conversion of existing, commonly-used yeast libraries or strains. We present Cas9-based, marker-less methodologies for (i) N-terminal tagging, (ii) C-terminally tagging yeast genes with 18 unique fusions, (iii) conversion of fluorescently-tagged strains into newly engineered (or codon optimized) variants, and finally, (iv) use of a Cas9 “gene drive” system to rapidly achieve a homozygous state for a hypomorphic query allele in a diploid strain. These CRISPR-based methods demonstrate use of targeting universal sequences previously introduced into a genome

Die Weltwirtschaft im Wandel: Symposium 1975

Seit einigen Jahren verstärkt sich der Eindruck, daß die Weltwirtschaft in einem raschen Wandel begriffen ist. Die Entwicklungsländer fordern eine neue Weltwirtschaftsordnung. Auf die weltweite Hochkonjunktur ist eine scharfe und recht lange anhaltende Rezession gefolgt; aber noch immer dominieren inflationäre Tendenzen. Den Konjunktureinbruch überlagert hat die Vervielfachung des Ölpreises Ende 1973. Sie wurde von vielen Beobachtern als Beginn einer Phase mit zunehmendem Rohstoffmangel und nie gekannten Ungleichgewichten in den Zahlungsbilanzen gedeutet. Von den vielfach geäußerten Befürchtungen haben sich manche als übertrieben erwiesen; denn viele Schwierigkeiten konnte der Markt viel besser als erwartet und ziemlich geräuschlos bewältigen. Gleichwohl erscheint es geraten, die alten und die neuen Probleme der Weltwirtschaft ständig zu beobachten. Vom 23. -27. Juni, traf sich in Kiel eine Gruppe unabhängiger Ökonomen aus Japan, Nordamerika und der Europäischen Gemeinschaft in der Absicht, die Situation der Weltwirtschaft zu analysieren und Vorschläge für wirtschaftspolitische Maßnahmen auf nationaler und internationaler Ebene auszuarbeiten. Das Institut für Weltwirtschaft war Gastgeber. Das Kieler Symposium ist ein Glied in einer Reihe trilateraler Konferenzen über weltwirtschaftliche Fragen, die von der Brookings Institution in Washington, dem Japan Economic Research Center in Tokio und dem Institut für Weltwirtschaft gemeinsam veranstaltet werden. Die Ergebnisse werden jeweils in englischer, japanischer und deutscher Sprache veröffentlicht; frühere Berichte in der deutschen Fassung sind als Kieler Diskussionsbeiträge Nr. 31 "Welthandel und Strukturanpassung in den Industrieländern" und Nr. 36 "Weltrohstoffversorgung: Konflikt oder Kooperation?" erschienen. --