118 research outputs found
Cell wall characteristics during sexual reproduction of Mougeotia sp. (Zygnematophyceae) revealed by electron microscopy, glycan microarrays and RAMAN spectroscopy
Mougeotia spp. collected from field samples were investigated for their conjugation morphology by light-, fluorescence-, scanning- and transmission electron microscopy. During a scalarifom conjugation, the extragametangial zygospores were initially surrounded by a thin cell wall that developed into a multi-layered zygospore wall. Maturing zygospores turned dark brown and were filled with storage compounds such as lipids and starch. While M. parvula had a smooth surface, M. disjuncta had a punctated surface structure and a prominent suture. The zygospore wall consisted of a polysaccharide rich endospore, followed by a thin layer with a lipid-like appaerance, a massive electron dense mesospore and a very thin exospore composed of polysaccharides. Glycan microarray analysis of zygospores of different developmental stages revealed the occurrence of pectins and hemicelluloses, mostly composed of homogalacturonan (HG), xyloglucans, xylans, arabino-galactan proteins and extensins. In situ localization by the probe OG7-13AF 488 labelled HG in young zygospore walls, vegetative filaments and most prominently in conjugation tubes and cross walls. Raman imaging showed the distribution of proteins, lipids, carbohydrates and aromatic components of the mature zygospore with a spatial resolution of ~ 250 nm. The carbohydrate nature of the endo- and exospore was confirmed and in-between an enrichment of lipids and aromatic components, probably algaenan or a sporopollenin-like material. Taken together, these results indicate that during zygospore formation, reorganizations of the cell walls occured, leading to a resistant and protective structure
Cellulose microfibril orientation of Picea abies and its variability at the micron-level determined by Raman imaging
The functional characteristics of plant cell walls depend on the composition of the cell wall polymers, as well as on their highly ordered architecture at scales from a few nanometres to several microns. Raman spectra of wood acquired with linear polarized laser light include information about polymer composition as well as the alignment of cellulose microfibrils with respect to the fibre axis (microfibril angle). By changing the laser polarization direction in 3° steps, the dependency between cellulose and laser orientation direction was investigated. Orientation-dependent changes of band height ratios and spectra were described by quadratic linear regression and partial least square regressions, respectively. Using the models and regressions with high coefficients of determination (R2 > 0.99) microfibril orientation was predicted in the S1 and S2 layers distinguished by the Raman imaging approach in cross-sections of spruce normal, opposite, and compression wood. The determined microfibril angle (MFA) in the different S2 layers ranged from 0° to 49.9° and was in coincidence with X-ray diffraction determination. With the prerequisite of geometric sample and laser alignment, exact MFA prediction can complete the picture of the chemical cell wall design gained by the Raman imaging approach at the micron level in all plant tissues
Insights into the chemical composition of Equisetum hyemale by high resolution Raman imaging
Equisetaceae has been of research interest for decades, as it is one of the oldest living plant families, and also due to its high accumulation of silica up to 25% dry wt. Aspects of silica deposition, its association with other biomolecules, as well as the chemical composition of the outer strengthening tissue still remain unclear. These questions were addressed by using high resolution (<1 μm) Confocal Raman microscopy. Two-dimensional spectral maps were acquired on cross sections of Equisetum hyemale and Raman images calculated by integrating over the intensity of characteristic spectral regions. This enabled direct visualization of differences in chemical composition and extraction of average spectra from defined regions for detailed analyses, including principal component analysis (PCA) and basis analysis (partial least square fit based on model spectra). Accumulation of silica was imaged in the knobs and in a thin layer below the cuticula. In the spectrum extracted from the knob region as main contributions, a broad band below 500 cm−1 attributed to amorphous silica, and a band at 976 cm−1 assigned to silanol groups, were found. From this, we concluded that these protrusions were almost pure amorphous, hydrated silica. No silanol group vibration was detected in the silicified epidermal layer below and association with pectin and hemicelluloses indicated. Pectin and hemicelluloses (glucomannan) were found in high levels in the epidermal layer and in a clearly distinguished outer part of the hypodermal sterome fibers. The inner part of the two-layered cells revealed as almost pure cellulose, oriented parallel along the fiber
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A millennium-long 'Blue Ring' chronology from the Spanish Pyrenees reveals severe ephemeral summer cooling after volcanic eruptions
Abstract
‘Blue Rings’ (BRs) are distinct wood anatomical anomalies recently discovered in several tree species from different sites. While it is evident that they are associated with a cooling-induced lack of cell wall lignification, BRs have yet to be evaluated systematically in paleoclimate studies. Here, we present a continuous wood anatomical assessment of 31 living and relict pine samples from a high-elevation site in the central Spanish Pyrenees that span the period 1150–2017 CE at annual resolution. While most BR years coincide with cold summer temperatures and many BRs follow large volcanic eruptions, some were formed during overall warm summers. We also see a differential response between eruptions: the Samalas eruption is followed by 80% BRs in 1258, but only a modest signal is evident after the 1815 Tambora eruption, and there are no wood anatomical effects of the Laki eruption in 1783–1784. Apparently linked to a cluster of tropical eruptions in 1695 and 1696 CE, 85% BRs occurred in 1698. This new wood anatomical evidence is corroborated by the record of sulphur deposition in polar ice cores, and corresponds with catastrophic famine and unprecedented mortality in Scotland. The extremely rare occurrence of consecutive BRs in 1345 and 1346 marks the onset and spread of the Black Death, Europe’s most devastating plague pandemic. In their ability to capture severe ephemeral cold spells, as short as several days or weeks, BR chronologies can help to investigate and understand the impacts of volcanism on climate and society.</jats:p
Self-assembly of amorphous calcium carbonate microlens arrays
Biological materials are often based on simple constituents and grown by the principle of self-assembly under ambient conditions. In particular, biomineralization approaches exploit efficient pathways of inorganic material synthesis. There is still a large gap between the complexity of natural systems and the practical utilization of bioinspired formation mechanisms. Here we describe a simple self-assembly route leading to a CaCO3 microlens array, somewhat reminiscent of the brittlestars' microlenses, with uniform size and focal length, by using a minimum number of components and equipment at ambient conditions. The formation mechanism of the amorphous CaCO3 microlens arrays was elucidated by confocal Raman spectroscopic imaging to be a two-step growth process mediated by the organic surfactant. CaCO3 microlens arrays are easy to fabricate, biocompatible and functional in amorphous or more stable crystalline forms. This shows that advanced optical materials can be generated by a simple mineral precipitation
In situ label-free imaging of hemicellulose in plant cell walls using stimulated Raman scattering microscopy
PHYSICAL, MORPHOLOGICAL PROPERTIES AND RAMAN SPECTROSCOPY OF CHESTNUT BLIGHT DISEASED CASTANEA SATIVA MILL. WOOD
Raman imaging of changes in the polysaccharides distribution in the cell wall during apple fruit development and senescence
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