Real time systems and public information

Using computer technology in future public information system

On comparison and administration - A philosophical discourse Internal working paper no. 1

Philosophical discourse on comparitive administratio

Technology and Urban Management. Semiannual Report, October 1, 1967 through March 31, 1968

The projects under Technology and Urban Management (TAUM) have continued during the last few months with considerable success. The individual studies conducted in the City of Oakland and the progress made are described in this report

Rapid creation and quantitative monitoring of high coverage shRNA libraries.

Short hairpin RNA libraries are limited by low efficacy of many shRNAs and by off-target effects, which give rise to false negatives and false positives, respectively. Here we present a strategy for rapidly creating expanded shRNA pools (approximately 30 shRNAs per gene) that are analyzed by deep sequencing (EXPAND). This approach enables identification of multiple effective target-specific shRNAs from a complex pool, allowing a rigorous statistical evaluation of true hits