Investigation of photoprotective, anti-inflammatory, antioxidant capacities and lc–esi–ms phenolic profile of astragalus gombiformis pomel

Plant-derived compounds have recently been gaining popularity as skincare factors due to their ability to absorb ultraviolet radiations and their anti-inflammatory, and antioxidant properties. In this light, this work aimed to evaluate in vitro the pharmacological activities of the butanolic extract prepared from the aerial parts of Astragalus gombiformis Pomel, an endemic species to southern Algeria. The sun protection factor was used to assess the photoprotective effect (SPF), the protein denaturation method to determine the anti-inflammatory activity, and brine shrimp nauplii and OxHLIA assay, respectively, to assess the cytotoxicity and antioxidant capacity of A. gombiformis. In addition, LC–ESI–MS analysis was employed for the characterization of the phenolic constituents of A. gombiformis. The results showed that A. gombiformis had high capacity for absorbing UV radiations with an SPF of 37.78 ± 0.85 and significant anti-inflammatory activity with a percentage inhibition of 75.38% which is close to that of diclofenac and ketoprofen. In addition, A. gombiformis was found to have effective cytotoxicity against Artemia nauplii with a DC50 value of about 44.7 µg/mL, but a weak hemolytic effect against human erythrocytes. LC–ESI–MS results detected the presence of 17 phenolic compounds with a predominance of cirsiliol, silymarin, quercitrin (quercetin-3-O-rhamnoside), and kaempferol. Taken together, these results suggest that A. gombiformis extract could be used as a skincare agent in cosmetic formulations, providing excellent antioxidant and anti-inflammatory protection, allowing the treatment of skin conditions, as well as a pharmaceutical agent with multidimensional applications.The authors are grateful to the Algerian Ministry of Higher Education and Scientific Research and the National Centre for Biotechnology Research (C.R.B.T) for their respective financial and material supports

A comparative study on chemical profile and biological activities of aerial parts (stems, flowers, leaves, pods and seeds) of Astragalus gombiformis

The present work aims to characterize the chemical profile of phenolic compounds and some biological activities of Astragalus gombiformis. The butanolic fractions of five aerial organs (stems, flowers, leaves, pods and seeds) were quantified and identified by LC–MS analysis. The results were revealed the presence of 13 phenolic compounds (quinic acid, p-coumaric acid, transfrulic acid, Hyperoside (quercetin-3-o-galactoside, Quercetrin (quercetin-3-o-rhamonoside), Apegenin-7-o-glucoside, kampherol, Naringenin, Apegenin, Luteolin, Cirsiliol, Cirsilineol and Acacetin. In terms of biological activities, the antioxidant,α-amylase inhibitory and anticholinesterase were determined. The butanolic extract from flowers showed the highest antioxidant activity in DPPH and ABTS(IC50: 16, 43 ± 0, 46 and 16, 13 ± 0, 35 μg/mL) very closer with standards tested and week activity with Galvinoxyl radical (GOR) (IC50: 583, 95 ± 2, 20 μg/mL), while other organ's extract exhibited moderate antioxidant activity. The leaves extract was found to exhibit the highest inhibitory effect against BChE (IC50: 165,54 ± 3,49 μg/mL) compared by the other parts which give a weak inhibitory effect at 200 μg/mL. A significant alpha-amylase inhibitory activity was displayed by seeds, leaves, pods and stems extracts (IC50:76.41 ± 3.72; 74.61 ± 3.68; 88.13 ± 1.81 and 83.81 ± 1.74 μg/mL) more the standard used. Based on these results, it is right to conclude that A. gombiformis is important source of the natural anti-diabetic, antioxidants and anti-Alzheimer′s disease.The authors are grateful to the Algerian Ministry of Higher Education and Scientific Research and the National Centre for Biotechnology Research (C.R.B.T) for their respective financial and material supports

Antioxidant Activity and Phenolic Content of Extracts of Wild Algerian Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes)

Mushrooms can be employed as functional food or medicines for prevention and treatment of several chronic diseases, since they are rich sources of bioactive metabolites. Nonetheless, the therapeutic value of Algerian mushrooms remains largely unexplored. There\u2019s no work concerning the different medicinal properties of the endemic Ganoderma lucidum in Algeria. The present study aims to compare the antioxidant potential of an endemic specimen collected at the forest of lake Tonga in the national park of EL Kala in North-East of Algeria with findings from other countries. To this end, the contents of phenolics and flavonoids were determined, using three solvents of increasing polarity (chloroform, ethyl acetate and butanol). The mushroom specimen was identified as G. lucidum based on strong molecular evidence using ITS, LSU and SSU sequences.Our study has shown that ethyl acetate extract contained higher total phenolic (171.1\ub11.06\u3bcg GAE/mg and 102.5\ub10.69 \u3bcg PE/mg extract) and flavonoid contents (25.48\ub10.13\u3bcg QE/mg and 40.45\ub10.83\u3bcg RE/mg extract) than in other mushroom extract (chloroform and butanolic). The ethyl acetate extract showed a good antioxidant activity in DPPH (28.51\ub10.24 \u3bcg/mL), ABTS\u2022+ (10.06\ub10.13 \u3bcg/mL), Galvinoxyl radical (15.46\ub10.48 \u3bcg/mL), Reducing power (22.74\ub10.30 \u3bcg/mL), CUPRAC (21.36\ub10.04 \u3bcg/mL) and Phenanthroline assay (12.87\ub10.26 \u3bcg/mL).These results suggest that G. lucidum could be used as a source of strong natural antioxidants for the food and pharmaceutical industries

Chemical composition, in vitro antioxidant, anticholinesterase and antibacterial activities of Linaria scariosa Desf

The study was performed on the dichloromethane (DCM), ethyl acetate (EAc) and n-butanol (Bu) fractions (F) obtained from the 80% ethanol extract of Linaria scariosa Desf. aerial parts, collected in the North Eastern region of Algeria. Remarkable total phenolic and flavonoid contents were obtained, mainly for EAcF. These results were in accordance with the antioxidant activity of EAcF against DPPH, ABTS, CUPRAC and reducing power tests. DCMF and BuF exhibited significant cholinesterase activity inhibition of BChE and AChE. Moreover, EAcF displayed only moderate antibacterial activities, especially against S. aureus. The biological results were correlated to the chemical components, deduced by both GC-MS analysis of the fractions and the isolation of hemipholin, pectolinarigenin, antirride, antirrinoside, pectolinarin and linariosise, some of which known to exhibit potent effects on the tested biological activities. The study provides the first biological and chemical investigation on Linaria scariosa Desf (unresolved name)

Interaction of a novel inorganic nickel complex with tyrosinase as potential inhibitor: Synthesis, spectroscopic, DFT, NBO, docking and ADMET properties

The obtained Ni(II) complex has been synthesized and characterized by elemental analysis (EA), spectroscopic studies such as FT-IR, UV-Vis. and MS. Also, thermal analysis (TG and DTG) analysis, X-ray powder diffractometry (PXRD) and morphological (SEM/EDXS) measurements were as well performed. By using cyclic voltammetry (CV), the redox properties of Ni(L) complex was found to be a quasi-reversible Ni(II)/Ni(I) redox system. The structure of Ni(L) complex was investigated by utilizing density functional theory (DFT) calculations with the Becke 3-Lee-Yang-Parr (B3LYP) method employing the LANL2DZ basis set. Frontier molecular orbitals (FMOs), important parameters of reactivity, Mulliken charge and Natural bond orbitals (NBOs) computational studies on Ni(L) complex were carried out in the ground state using DFT theory at B3LYP/ LANL2DZ level. This complex was found to exhibit, in vitro, a strong tyrosinase inhibitory activity with an IC value of 5.7 μM approximately 6.5-fold better than kojic acid as positive control IC = 37.86 µM). The binding energy of the best pose, in silico, is – 8.7 kcal/mol. Thus, these results showed a good agreement with the experimental data obtained from the enzyme-inhibiting assay.The authors thank the Algerian Ministry of Higher Education and Scientific Research (MESRS) and the General Directorate for Scientific Research and Technological Development (DGRSDT) for the financial support