Fermi detected blazars seen by INTEGRAL

Multiwavelength observations are essential to constrain physical parameters of the blazars observed by Fermi/LAT. Among the 187 AGN significantly detected in public INTEGRAL data above 20 keV by the imager IBIS/ISGRI, 20 blazars were detected. 15 of these sources allowed significant spectral extraction. They show hard X-ray spectra with an average photon index of 2.1+-0.1 and a hard X-ray luminosity of L(20-100 keV) = 1.3e46 erg/s. 15 of the INTEGRAL blazars are also visible in the first 16 months of the Fermi/LAT data, thus allowing to constrain the inverse Compton branch in these cases. Among others, we analyse the LAT data of four blazars which were not included in the Fermi LAT Bright AGN Sample based on the first 3 months of the mission: QSO B0836+710, H 1426+428, RX J1924.8-2914, and PKS 2149-306. Especially for blazars during bright outbursts, as already observed simultaneously by INTEGRAL and Fermi (e.g. 3C 454.3 and Mrk 421), INTEGRAL provides unique spectral coverage up to several hundred keV. We present the spectral analysis of INTEGRAL and Fermi data and demonstrate the potential of INTEGRAL observations of Fermi detected blazars in outburst by analysing the combined data set of the persistent radio galaxy Cen A.Comment: 5 pages, 5 figures, 2009 Fermi Symposium, eConf Proceedings C09112

Compton processes in the bright AGN MCG+8-11-11

We present preliminary results on the hard X-ray emission properties of the Seyfert 1.5 galaxy MCG+8-11-11 as observed by INTEGRAL and SWIFT. All the INTEGRAL IBIS/ISGRI data available up to October 2009 have been analyzed together with two SWIFT/XRT snapshot observations performed in August and October 2009, quasi-simultaneously to INTEGRAL pointed observations of MCG+8-11-11. No correlation is observed between the hard X-ray flux and the spectral slope, while the position of the high-energy cut-off is found to have varied during the INTEGRAL observations. This points to a change in the temperature of the Comptonising medium from a minimum value of kT = 30-50 keV to values larger than 100-150 keV. There is no significant detection of Compton reflection, with a 3 sigma upper limit of R < 0.2, and no line has been detected at 112 keV, as previously claimed from HEAT observations (112 keV flux F < 2.4e-4 ph/cm^2/s). The variability behaviour of MCG+8-11-11 is found to be similar to that shown by IC 4329A, with different temperatures of the electron plasma for similar flux levels of the source, while other bright Seyfert galaxies present different variability patterns at hard X-rays, with spectral changes correlated to flux variations (e.g. NGC 4151).Comment: 6 pages, 4 figures. Accepted for publication on PoS (contribution PoS(INTEGRAL 2010)077), proceedings of the 8th INTEGRAL Workshop "The Restless Gamma-ray Universe" (September 2010, Dublin, Ireland

Paving the way for transgenic schistosomes

In parasitological research, significant progress has been made with respect to genomics and transcriptomics but transgenic systems for functional gene analyses are mainly restricted to the protozoan field. Gene insertion and knockout strategies can be applied to parasitic protozoa as well as gene silencing by RNA interference (RNAi). By contrast, research on parasitic helminthes still lags behind. Along with the major advances in genome and transcriptome analyses e.g. for schistosomes, methods for the functional characterization of genes of interest are still in their initial phase and have to be elaborated now, at the beginning of the post-genomic era. In this review we will summarize attempts made in the last decade regarding the establishment of protocols to transiently and stably transform or transfect schistosomes. Besides approaches using particle bombardment, electroporation or virus-based infection strateies to introduce DNA constructs into adult and larval schistosome stages to express reporter genes, first approaches have also been made in establishing protocols based on soaking, lipofection, and/or electroporation for RNA interference to silence gene activity. Although in these cases remarkable progress can be seen, the schistosome community eagerly awaits major breakthroughs especially with respect to stable transformation, but also for silencing or knock-down strategies for every schistosome gene of interest