Recessive Antimorphic Alleles Overcome Functionally Redundant Loci to Reveal TSO1 Function in Arabidopsis Flowers and Meristems

Arabidopsis TSO1 encodes a protein with conserved CXC domains known to bind DNA and is homologous to animal proteins that function in chromatin complexes. tso1 mutants fall into two classes due to their distinct phenotypes. Class I, represented by two different missense mutations in the CXC domain, leads to failure in floral organ development, sterility, and fasciated inflorescence meristems. Class II, represented by a nonsense mutation and a T-DNA insertion line, develops wild-type–like flowers and inflorescences but shows severely reduced fertility. The phenotypic variability of tso1 alleles presents challenges in determining the true function of TSO1. In this study, we use artificial microRNA, double mutant analysis, and bimolecular fluorescence complementation assay to investigate the molecular basis underlying these two distinct classes of phenotypes. We show that the class I mutants could be converted into class II by artificial microRNA knockdown of the tso1 mutant transcript, suggesting that class I alleles produce antimorphic mutant proteins that interfere with functionally redundant loci. We identified one such redundant factor coded by the closely related TSO1 homolog SOL2. We show that the class I phenotype can be mimicked by knocking out both TSO1 and its homolog SOL2 in double mutants. Such antimorphic alleles targeting redundant factors are likely prevalent in Arabidopsis and maybe common in organisms with many sets of paralogous genes such as human. Our data challenge the conventional view that recessive alleles are always hypomorphic or null and that antimorphic alleles are always dominant. This study shows that recessive alleles can also be antimorphic and can produce a phenotype more severe than null by interfering with the function of related loci. This finding adds a new paradigm to classical genetic concepts, with important implications for future genetic studies both in basic research as well as in agriculture and medicine

Pregled znanstvenih napredaka u učinskoj elektronici usmjerenih ka osiguravanju efikasnog rada i dužeg životnog vijeka PEMgorivih ćelija

This article focuses on the main issues that affect the lifetime and performance of proton-exchange membrane fuel cells. The short lifespans of these fuel cells represent a barrier to their massive commercialization and usage in mobile and stationary applications. As fuel cell is a very complex system, a lot of knowledge of different areas is required, such as chemistry, electricity and mechanics, in order to completely understand its operation and all the problems that can occur during it. It is for this reason that an interdisciplinary approach needs to be taken when designing fuel-cell energy systems. This paper focuses on identifying and solving those issues that negatively affect the lifetime and performance of fuel cells. It is hoped that this article would be a valuable aid for power electronics’ researchers and engineers for better understanding the presented issues and a useful guide for solving them with the use of proper power electronic-devices. Initially, the basic operation and structure of a proton-exchange membrane fuel cell is explained. Three main issues that can occur during operation of a mobile or stationary fuel cell energy system are pointed out and discussed in details, on the basis of the state-of-the-art on fuel cell technology. These issues are poor water management, reactant gas starvation and fuel cell current ripple. This article provides answers as to why they occur, how they affect the fuel cell, how they can be mitigated, and what are the future trends within this research field.Članak se osvrće na ključna pitanja koja utječu na vrijeme rada i performanse gorivih ćelija s polimernom membranom kao elektrolitom. Kratak životni vijek gorivih ćelija takve vrste prepreka je njihovoj komercijalizaciji i masovnoj upotrebi u mobilnim i stacionarnim stanicama. Budući da su gorive ćelije komplicirani sustavi potrebno je znanje iz raznih područja kemije, elektrotehnike i mehanike da bi se u potpunosti mogao razumjeti njihov način rada i problemi koji se događaju. Upravo je zbog toga multidisciplinarni pristup nužnost pri razvoju sustava koji koriste gorive ćelije. Ovaj je članak usmjeren prema identifikaciji i rješavanju onih problema koji negativno utječu na životni vijek i performanse gorivih ćelija. Autori se nadaju da će se članak pokazati kao korisna pomoć i vodič istraživačima i inženjerima u domeni učinske elektronike pri susretu s navedenim problemima. Objašnjen je način rada i struktura gorive ćelije s polimernom membranom kao elektrolitom. Izložena su, i diskutirana do u detalje, tri glavna problema sa stajališta trenutačnih spoznaja u području učinske elektronike. Ti problemi su: loše upravljanje vodom, nestanak reaktantnog plina i strujni trzaji u gorivim ćelijama. Objašnjeno je zašto se ovi problemi događaju, kako utječu na gorivu ćeliju, kako ih se može spriječiti i koje su buduće perspektive istraživanja

Perturb and Observe MPPT-algorithm with a Current Controller based on the Sliding Mode

In this paper a current controller is used to reject the 100-Hz oscillation of the voltage of the photovoltaic field taking place in any grid connected system. With respect to the classical linear voltage control the current-based sliding-mode approach ensures a wider regulation band but, unfortunately, in presence of a fast irradiance variation the sliding conditions might be violated, so that the operating point, which should be the maximum power point, might not be the expected one. In order to prevent such a drawback, an appropriate voltage compensation loop is used to interface the current-based sliding mode with the Maximum Power Point Tracking algorithm. Simulation and experimental results confirm the goodness of the proposed algorithm

A fast current-based MPPT technique based on sliding mode control

This paper introduces a novel maximum power point tracking technique aimed at maximizing the power produced by photovoltaic systems. The largest part of the approaches presented in literature are based on the sensing of the photovoltaic generator voltage. On the contrary, in this paper a current-based technique is proposed: the sensing of the current in the capacitor placed in parallel with the photovoltaic source is one of the innovative aspects of the proposal. A dual control loop based on the sliding mode control ensures a very fast tracking of irradiation variations. Features of the proposed algorithm are supported by a theoretical analysis and simulations. The technique described in this paper is patent pending

A fast current-based MPPT technique based on sliding mode control

This paper introduces a novel maximum power point tracking (MPPT) technique aimed at maximizing the power produced by photovoltaic (PV) systems. The largest part of the MPPT approaches presented in the literature are based on the sensing of the PV generator voltage. On the contrary, in this paper, a current-based technique is proposed: the sensing of the current in the capacitor placed in parallel with the PV source is one of the innovative aspects of the proposal. A dual control technique based on an inner current loop plus an outer voltage loop allows to take profit of the fast current tracking capability of the inner current loop while the voltage loop benefits from the logarithmic dependency of the PV voltage on the irradiation level. The features of the proposed algorithm, particularly in terms of tracking of irradiation variations and disturbance rejection, are supported by theoretical analysis, simulations, and experimental results. The technique described in this paper is patent pending

Absence of magnetic order in Yb₃Ga₅O₁₂: Relation between phase transition and entropy in geometrically frustrated materials

From muon spin relaxation spectroscopy experiments, we show that the sharp peak (lambda type anomaly) detected by specific heat measurements at 54 mK for the ytterbium gallium garnet compound, Yb3Ga5O12, does not correspond to the onset of a magnetic phase transition, but to a pronounced building up of dynamical magnetic pair-correlations. Beside the lambda anomaly, a broad hump is observed at higher temperature in the specific heat of this garnet and other geometrically frustrated compounds. Comparing with other frustrated magnetic systems we infer that a ground state with long-range order is reached only when at least 1/4-1/3 of the magnetic entropy is released at the lambda transition.Comment: 5 pages, 2 figure

Photovoltaic module cascaded converters for distributed maximum power point tracking: A review

© The Institution of Engineering and Technology 2020 Operating photovoltaic (PV) systems under partial shading conditions results in significant power losses. To mitigate partial shading effects, distributed maximum power point tracking (DMPPT) architectures have been proposed. An emerging DMPPT technique represented by PV module cascaded converters (MCCs) has been widely reported in the literature. In this architecture, a DC converter is allocated for each PV module to process and maximise its power. In this sense, mismatch effects are mitigated between PV modules. While MCC architecture has prominent advantages and value-added features, its challenges and limitations cannot be ignored. This study presents a comprehensive review of the state of the art of PV MCC architecture to help readers realise the progress of this DMPPT technique. Several points are extensively discussed and analysed including concept realisation and analysis, DC converter topologies and design optimisation, DMPPT performance limitations, DMPPT control, and protection. The main concepts are reemphasised through a set of simulations. Finally, a list of potential research areas in this field is introduced