Impacts of blockchain technology in agrifood: exploring the interplay between transactions and firms’ strategic resources

Purpose: Despite the growing interest in blockchain technology (BCT) applications in the agri-food industry, evidence of their economic and strategic implications remains scarce. This study aims to contribute to filling this gap by jointly investigating how BCT adoption affects transactional relationships, and how it contributes to the firm’s strategic resources. Design/methodology/approach: An explanatory case study is conducted based on a theoretical framework grounded on transaction cost economics and the resource-based-dynamic capabilities view. Six BCT implementations by agri-food firms are studied. Data were collected through semi-structured interviews and analysed using thematic analysis. Findings: Findings reveal that BCT benefits depend on how companies integrate technology across their supply chains. In fact, the results suggest that overall transaction efficiency within the supply chain is enhanced only for those firms prioritising stakeholder engagement during technology implementation and leveraging existing trust relationships with economic agents. Moreover, the results suggest that BCT is not yet perceived as a strategic resource, but rather that it has the potential to enhance firms’ operational-adaptive, absorptive and innovative capabilities. When all supply chain actors clearly understand blockchain’s functionality and value, the development of these capabilities becomes more pronounced. Practical implications: The study identifies two BCT adoption configurations. One primarily focuses on enhancing supply chain efficiency and transparency (dynamic BCT), while the other uses BCT mainly for marketing purposes (static BCT). These configurations lead to varied possibilities for leveraging BCT’s potential advantages. Furthermore, they show how a mismatch between a strategic approach and its chosen configuration could work against any positive impact and lead to disillusionment with the BCT. Thus, managers should assess carefully the impact of such different configuration choices on performance. Originality/value: To the best of the authors’ knowledge, this is the first study to attempt to analyse the economic implications of adopting BCT in the food sector from both a firm and supply chain perspective. Additionally, it shows how interpreting these impacts is contingent on the diverse modalities for embedding BCT into existing supply chains

EZETIMIBE PROTECTS THP-1 CELLS FROM ISCHEMIA-REPERFUSION INJURY REDUCING OXIDATIVE STRESS AND UP-REGULATING NRF2/ ARE GENE EXPRESSION

Background and Aims: We demonstrated that physical training, characterized by repeated ischemia-reperfusion (I-R) episodes (ischemic conditioning, IC), protects circulating cells from peripheral artery disease (PAD) patients against ischemic harms by reducing oxidative stress (OS) and by up-regulating nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) pathway expression. Ezetimibe (Eze) has been shown to alleviate OS enhancing Nrf2 nuclear translocation in an AMPK/p62-dependent manner. In a cellular I-R and IC model, we aimed to investigate: 1) the effect of Eze on OS and Nrf2/ARE gene expression 2) whether Eze could have a synergistic effect on IC. Methods: THP-1 cells were treated with or without Eze (50mM) overnight, then subjected to 1 or 6 repetitive I-R cycles using EVOS FL Auto Imaging System. Reactive oxygen species (ROS) formation was evaluated with DCF in cytofluorimetry. Nrf2/ARE and p62 gene expression were evaluated by RT-PCR and western blotting. Results: When THP-1 cells were exposed to 1 I-R cycle, the preincubation with Eze significantly reduced ROS formation (p<0.01) and up-regulated Nrf2/ARE pathway expression and p62 phosphorylation (p<0.001). Multiple I-R cycles, acting as IC, significantly reduced ROS formation and upregulated Nrf2/ARE gene expression (p<0.001); in these conditions, Eze preincubation was able not only to almost abolish ROS formation (p<0.01) but also further up-regulate Nrf2/ARE expression. Conclusions: In our I-R model, Eze not only restores I-R-induced oxidative damages through Nrf2/ARE signaling up-regulation but also has a synergistic effect on IC. This new \u201cpleiotropic\u201d effect, if confirmed in vivo, may strengthen the use of Eze in PAD patien

Frequency of electrophoretic changes consistent with feline infectious peritonitis in two different time periods (2004-2009 vs 2013-2014)

OBJECTIVES: The aim of this study was to evaluate whether the frequency of electrophoretic changes in serum of cats with feline infectious peritonitis (FIP) changed in recent years vs past years. METHODS: Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) from cats with FIP and healthy cats recorded in the periods 2004-2009 and 2013-2014 were retrospectively analysed. Relative and absolute values of each electrophoretic fraction were recorded and the number of cats showing single or combined electrophoretic changes consistent with FIP (hypoalbuminaemia, inverted albumin to globulin [A:G] ratio, increased total protein, total globulin, alpha (α)2-globulin and gamma (γ)-globulin concentration) were counted. Additionally, a visual analysis of electrophoretograms was also performed. Results for the two time periods were statistically compared. RESULTS: The details of 91 AGE procedures (41 from cats with FIP and 50 from healthy cats) and 45 CZE procedures (26 from cats with FIP and 19 from healthy cats) were obtained from the database. No significant differences between the two time periods were found both in FIP and in healthy cats analysed with CZE and in healthy cats analysed with AGE. Compared with 2004-2009, cats with FIP sampled in 2013-2014 with AGE showed a significantly lower concentration of total protein, γ-globulins and total globulins, and a significantly higher A:G ratio and percentage of albumin and α2-globulins. Using both AGE and CZE, in recent years the proportion of cats with high α2-globulins without gammopathy and the proportion of cats with gammopathy alone decreased. With a visual approach, the number of patterns considered as dubious increased in the second period with AGE (non-statistically significant). CONCLUSIONS AND RELEVANCE: The frequency of electrophoretic abnormalities in cats with FIP decreased in recent years, independently of the technique employed. Although the mechanism responsible for this change was not investigated in this study, this altered frequency may decrease the diagnostic accuracy of serum protein electrophoresis for FIP

Adopting environmentally-friendly certifications : Transaction cost and capabilities perspectives within the Italian wine supply chain

Purpose–Environmentally-friendly certifications have increased over the past decade within food supply chains. Although a large body of literaturehas explored the drivers leadingfirms to adopt such certifications, it has not closely examined the strategic motivations associated with theiradoption. This paper aims to investigate an environmentally-friendly certification, VIVA, examining its role as an alternative form of supply chaingovernance. The aim is to investigate the drivers affecting the adoption of VIVA and to assess managerial perceptions related to transaction-relatedcharacteristics and thefirm’s internal resources and capabilities. Design/methodology/approach–This study draws upon both an extended transaction cost economics perspective, which is based on transactionrisks and the resource-based view, which examines afirm’s internal resources. A survey was conducted via a structured questionnaire sent to all ofthe wine producers in charge of the decision regarding whether to adopt VIVA certification. A Hierarchal Bayesian Model was applied to analysequestionnaire responses. Such a model allows us to specify the probabilistic relationship between questions and latent constructs and to carry overuncertainty across modelling levels. Findings–The adoption of this environmentally-friendly certification is envisioned as a tool to curb internal risks, and thus to manage behaviouraluncertainty within the supply chain. A high level of exposure to exogenous transaction risks discouragesfirms from adopting VIVA certification. Thecertification system is not perceived as a promoter of operational capabilities. Managers are more likely to implement the certification when theyexpect that its adoption will leverage their potential knowledge of the supply chain or prompt new and better collaborations with the suppliers.Therefore, the certification can become a resource that interacts with the capabilities of thefirm, expressing complementarities that stimulate theformation of dynamic capabilities.Research limitations/implications–The identification of drivers from the two theoretical perspectives offers insights into the attributes that areperceived as important by managers and which, therefore, could be leveraged to foster the adoption of the environmental certification. The externalvalidity of the study could be improved by extending the sample to other certifications and supply chains. Originality/value–The study offers a different perspective on environmental certification. It demonstrates that considering the certification as analternative form of supply chain governance opens up a set of efficiency and strategic considerations that could be addressed to promote theeffectiveness of an environmental strategy within a supply cha

Carbon footprint information, prices, and restaurant wine choices by customers : A natural field experiment

Growing evidence suggests that consumers should be involved in the solutions to climate change. To test the responsiveness of wine consumers to carbon footprint stimuli we set a natural field experiment in a restaurant modifying the choice architecture of wine decisions. We modified wine cards to provide different information and price incentives to consumers to test the effects of two policy interventions for reducing CO2 emissions. Specifically, we test the provision of carbon footprint information and an additional price for carbon emissions. We randomly assigned subjects to four different wine cards. Results show that carbon footprint information alone is not enough to affect wine choices, but its effect becomes significant when combined with a price change. A card showing the carbon footprint of wine bottles and proportionally higher wine prices was associated with choices leading to lower carbon emissions. However, when information about the additional price to offset wine carbon emissions was made explicitly visible to consumers on another card, an opposite effect of an increase in the choice of wines with higher carbon-emitting production processes was observed. This finding indicates that how the price increase is presented can affect wine choices, highlighting the importance of careful information policy design

Ezetimibe prevents ischemia/reperfusion-induced oxidative stress and up-regulates Nrf2/ARE and UPR signaling pathways

BACKGROUND: While reperfusion is crucial for survival after an episode of ischemia, it also causes oxidative stress. Nuclear factor-E2-related factor 2 (Nrf2) and unfolded protein response (UPR) are protective against oxidative stress and endoplasmic reticulum (ER) stress. Ezetimibe, a cholesterol absorption inhibitor, has been shown to activate the AMP-activated protein kinase (AMPK)/Nrf2 pathway. In this study we evaluated whether Ezetimibe affects oxidative stress and Nrf2 and UPR gene expression in cellular models of ischemia-reperfusion (IR). METHODS: Cultured cells were subjected to simulated IR with or without Ezetimibe. RESULTS: IR significantly increased reactive oxygen species (ROS) production and the percentage of apoptotic cells without the up-regulation of Nrf2, of the related antioxidant response element (ARE) gene expression or of the pro-survival UPR activating transcription factor 6 (ATF6) gene, whereas it significantly increased the pro-apoptotic CCAAT-enhancer-binding protein homologous protein (CHOP). Ezetimibe significantly decreased the cellular ROS formation and apoptosis induced by IR. These effects were paralleled by the up-regulation of Nrf2/ARE and ATF6 gene expression and by a down-regulation of CHOP. We also found that Nrf2 activation was dependent on AMPK, since Compound C, a pan inhibitor of p-AMPK, blunted the activation of Nrf2. CONCLUSIONS: Ezetimibe counteracts IR-induced oxidative stress and induces Nrf2 and UPR pathway activation

Do dogs and cats passively carry sars-cov-2 on hair and pads?

The epidemiological role of domestic animals in the spread and transmission of SARS-CoV-2 to humans has been investigated in recent reports, but some aspects need to be further clarified. To date, only in rare cases have dogs and cats living with COVID-19 patients been found to harbour SARS-CoV-2, with no evidence of pet-to-human transmission. The aim of the present study was to verify whether dogs and cats act as passive mechanical carriers of SARS-CoV-2 when they live in close contact with COVID-19 patients. Cutaneous and interdigital swabs collected from 48 dogs and 15 cats owned by COVID-19 patients were tested for SARS-CoV-2 by qRT-PCR. The time elapsed between owner swab positivity and sample collection from pets ranged from 1 to 72 days, with a median time of 23 days for dogs and 39 days for cats. All samples tested negative, suggesting that pets do not passively carry SARS-CoV-2 on their hair and pads, and thus they likely do not play an important role in the virus transmission to humans. This data may contribute to confirming that the direct contact with the hair and pads of pets does not represent a route for the transmission of SARS-CoV-2

Flow cytometric features of lymphoid subsets in healthy and diseased cats

Introduction: Flow cytometry (FC) is widely used in humans and dogs to diagnose and characterize hematopoietic neoplasms. Conversely, its use in feline patients is still limited, leading to a lack of standardized protocols and subjective data interpretation. Methods: Herein, we describe FC features of circulating lymphoid subsets in a total of 20 cats: 9 healthy cats, 6 diseased cats without hematopoietic neoplasm, and 5 cats with probable chronic lymphocytic leukemia (CLL), using a panel of 10 antibodies and a multicolor approach, in terms of both cell size (nFSC) and degree of antigen expression (MFI). Results: Three main subsets were identified in healthy cats and diseased cats without hematopoietic neoplasm (namely, CD5 + CD45R-, CD21 + CD45R + and CD5 + CD45R+). CD4 + CD8- cells outnumbered CD4- CD8 + cells. Low percentages of CD4 + CD8 + and CD134 + cells were also present. MHCII had higher fluorescence intensity in B- than in T-cells. CD9 was not expressed on leukocytes surface, but on small events possibly referable to platelet clumps. In diseased cats without hematopoietic neoplasm, each T-cell subset was larger in size than in healthy cats. Finally, in cats with probable CLL the leading phenotype was CD5 + CD45R-CD4 + CD8-CD134 + MHCII+ and cell size overlapped with the one of the other diseased cats. Discussion: Our results are expected to lay the ground for a more standardized approach to feline samples for FC, and a more objective data interpretation, ultimately leading to improved diagnostic accuracy. Further studies are needed to assess the biological, diagnostic and prognostic value of specific FC patterns in feline medicine